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A traditional poly herbal medicine “Le Pana Guliya” induces apoptosis in HepG(2) and HeLa cells but not in CC1 cells: an in vitro assessment

“Le Pana Guliya” (LPG) is a polyherbal formulation which is used to treat different types of cancers in traditional medicine. In this study we describe in vitro efficacy and mechanism of action of LPG on two cancer cell lines (HepG(2) and HeLa) compared with a normal cell line CC1. The MTT, LDH assa...

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Autores principales: Wageesha, Nekadage Don Amal, Soysa, Preethi, Atthanayake, Keerthi, Choudhary, Muhammad Iqbal, Ekanayake, Mahinda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215177/
https://www.ncbi.nlm.nih.gov/pubmed/28101129
http://dx.doi.org/10.1186/s13065-016-0234-4
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author Wageesha, Nekadage Don Amal
Soysa, Preethi
Atthanayake, Keerthi
Choudhary, Muhammad Iqbal
Ekanayake, Mahinda
author_facet Wageesha, Nekadage Don Amal
Soysa, Preethi
Atthanayake, Keerthi
Choudhary, Muhammad Iqbal
Ekanayake, Mahinda
author_sort Wageesha, Nekadage Don Amal
collection PubMed
description “Le Pana Guliya” (LPG) is a polyherbal formulation which is used to treat different types of cancers in traditional medicine. In this study we describe in vitro efficacy and mechanism of action of LPG on two cancer cell lines (HepG(2) and HeLa) compared with a normal cell line CC1. The MTT, LDH assays and protein synthesis were used to study antiproliferative activity of LPG while NO synthesis and GSH content were assayed to determine the oxidative stress exerted by LPG. Rhodamine 123 staining, caspase 3 activity, DNA fragmentation and microscopic examination of cells stained with ethidium bromide/acridine orange were used to identify the apoptosis mechanisms associated with LPG. The LPG showed the most potent antiproliferative effect against the proliferation of HepG(2) and HeLa cells with an EC(50) value of 2.72 ± 1.36 and 19.03 ± 2.63 µg/mL for MTT assay after 24 h treatment respectively. In contrast, CC1 cells showed an EC(50) value of 213.07 ± 7.71 µg/mL. Similar results were observed for LDH release. A dose dependent decrease in protein synthesis was shown in both cancer cell types compared to CC1 cells. The reduction of GSH content and elevation of cell survival with exogenous GSH prove that the LPG act via induction of oxidative stress. LPG also stimulates the production of NO and mediates oxidative stress. Rhodamine 123 assay shows the mitochondrial involvement in cell death by depletion of Δψ inducing downstream events in apoptosis. This results in increase in caspase-3 activity eventually DNA fragmentation and LPG induced apoptotic cell death. In conclusion the present study suggested that the LPG exerted an anticancer activity via oxidative stress dependent apoptosis. Therefore present study provides the scientific proof of the traditional knowledge in using LPG as an anticancer agent.
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spelling pubmed-52151772017-01-18 A traditional poly herbal medicine “Le Pana Guliya” induces apoptosis in HepG(2) and HeLa cells but not in CC1 cells: an in vitro assessment Wageesha, Nekadage Don Amal Soysa, Preethi Atthanayake, Keerthi Choudhary, Muhammad Iqbal Ekanayake, Mahinda Chem Cent J Research Article “Le Pana Guliya” (LPG) is a polyherbal formulation which is used to treat different types of cancers in traditional medicine. In this study we describe in vitro efficacy and mechanism of action of LPG on two cancer cell lines (HepG(2) and HeLa) compared with a normal cell line CC1. The MTT, LDH assays and protein synthesis were used to study antiproliferative activity of LPG while NO synthesis and GSH content were assayed to determine the oxidative stress exerted by LPG. Rhodamine 123 staining, caspase 3 activity, DNA fragmentation and microscopic examination of cells stained with ethidium bromide/acridine orange were used to identify the apoptosis mechanisms associated with LPG. The LPG showed the most potent antiproliferative effect against the proliferation of HepG(2) and HeLa cells with an EC(50) value of 2.72 ± 1.36 and 19.03 ± 2.63 µg/mL for MTT assay after 24 h treatment respectively. In contrast, CC1 cells showed an EC(50) value of 213.07 ± 7.71 µg/mL. Similar results were observed for LDH release. A dose dependent decrease in protein synthesis was shown in both cancer cell types compared to CC1 cells. The reduction of GSH content and elevation of cell survival with exogenous GSH prove that the LPG act via induction of oxidative stress. LPG also stimulates the production of NO and mediates oxidative stress. Rhodamine 123 assay shows the mitochondrial involvement in cell death by depletion of Δψ inducing downstream events in apoptosis. This results in increase in caspase-3 activity eventually DNA fragmentation and LPG induced apoptotic cell death. In conclusion the present study suggested that the LPG exerted an anticancer activity via oxidative stress dependent apoptosis. Therefore present study provides the scientific proof of the traditional knowledge in using LPG as an anticancer agent. Springer International Publishing 2017-01-03 /pmc/articles/PMC5215177/ /pubmed/28101129 http://dx.doi.org/10.1186/s13065-016-0234-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wageesha, Nekadage Don Amal
Soysa, Preethi
Atthanayake, Keerthi
Choudhary, Muhammad Iqbal
Ekanayake, Mahinda
A traditional poly herbal medicine “Le Pana Guliya” induces apoptosis in HepG(2) and HeLa cells but not in CC1 cells: an in vitro assessment
title A traditional poly herbal medicine “Le Pana Guliya” induces apoptosis in HepG(2) and HeLa cells but not in CC1 cells: an in vitro assessment
title_full A traditional poly herbal medicine “Le Pana Guliya” induces apoptosis in HepG(2) and HeLa cells but not in CC1 cells: an in vitro assessment
title_fullStr A traditional poly herbal medicine “Le Pana Guliya” induces apoptosis in HepG(2) and HeLa cells but not in CC1 cells: an in vitro assessment
title_full_unstemmed A traditional poly herbal medicine “Le Pana Guliya” induces apoptosis in HepG(2) and HeLa cells but not in CC1 cells: an in vitro assessment
title_short A traditional poly herbal medicine “Le Pana Guliya” induces apoptosis in HepG(2) and HeLa cells but not in CC1 cells: an in vitro assessment
title_sort traditional poly herbal medicine “le pana guliya” induces apoptosis in hepg(2) and hela cells but not in cc1 cells: an in vitro assessment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215177/
https://www.ncbi.nlm.nih.gov/pubmed/28101129
http://dx.doi.org/10.1186/s13065-016-0234-4
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