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Immunohistochemical expression of intrarenal renin angiotensin system components in response to tempol in rats fed a high salt diet
AIM: To determine the effect of tempol in normal rats fed high salt on arterial pressure and the balance between antagonist components of the renal renin-angiotensin system. METHODS: Sprague-Dawley rats were fed with 8% NaCl high-salt (HS) or 0.4% NaCl (normal-salt, NS) diet for 3 wk, with or withou...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215206/ https://www.ncbi.nlm.nih.gov/pubmed/28101449 http://dx.doi.org/10.5527/wjn.v6.i1.29 |
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author | Cao, Gabriel Della Penna, Silvana Lorena Kouyoumdzian, Nicolás Martín Choi, Marcelo Roberto Gorzalczany, Susana Fernández, Belisario Enrique Toblli, Jorge Eduardo Rosón, María Inés |
author_facet | Cao, Gabriel Della Penna, Silvana Lorena Kouyoumdzian, Nicolás Martín Choi, Marcelo Roberto Gorzalczany, Susana Fernández, Belisario Enrique Toblli, Jorge Eduardo Rosón, María Inés |
author_sort | Cao, Gabriel |
collection | PubMed |
description | AIM: To determine the effect of tempol in normal rats fed high salt on arterial pressure and the balance between antagonist components of the renal renin-angiotensin system. METHODS: Sprague-Dawley rats were fed with 8% NaCl high-salt (HS) or 0.4% NaCl (normal-salt, NS) diet for 3 wk, with or without tempol (T) (1 mmol/L, administered in drinking water). Mean arterial pressure (MAP), glomerular filtration rate (GFR), and urinary sodium excretion (UVNa) were measured. We evaluated angiotensin II (Ang II), angiotensin 1-7 (Ang 1-7), angiotensin converting enzyme 2 (ACE2), mas receptor (MasR), angiotensin type 1 receptor (AT1R) and angiotensin type 2 receptor (AT2R) in renal tissues by immunohistochemistry. RESULTS: The intake of high sodium produced a slight but significant increase in MAP and differentially regulated components of the renal renin-angiotensin system (RAS). This included an increase in Ang II and AT1R, and decrease in ACE-2 staining intensity using immunohistochemistry. Antioxidant supplementation with tempol increased natriuresis and GFR, prevented changes in blood pressure and reversed the imbalance of renal RAS components. This includes a decrease in Ang II and AT1R, as increase in AT2, ACE2, Ang (1-7) and MasR staining intensity using immunohistochemistry. In addition, the natriuretic effects of tempol were observed in NS-T group, which showed an increased staining intensity of AT2, ACE2, Ang (1-7) and MasR. CONCLUSION: These findings suggest that a high salt diet leads to changes in the homeostasis and balance between opposing components of the renal RAS in hypertension to favour an increase in Ang II. Chronic antioxidant supplementation can modulate the balance between the natriuretic and antinatriuretic components of the renal RAS. |
format | Online Article Text |
id | pubmed-5215206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-52152062017-01-19 Immunohistochemical expression of intrarenal renin angiotensin system components in response to tempol in rats fed a high salt diet Cao, Gabriel Della Penna, Silvana Lorena Kouyoumdzian, Nicolás Martín Choi, Marcelo Roberto Gorzalczany, Susana Fernández, Belisario Enrique Toblli, Jorge Eduardo Rosón, María Inés World J Nephrol Basic Study AIM: To determine the effect of tempol in normal rats fed high salt on arterial pressure and the balance between antagonist components of the renal renin-angiotensin system. METHODS: Sprague-Dawley rats were fed with 8% NaCl high-salt (HS) or 0.4% NaCl (normal-salt, NS) diet for 3 wk, with or without tempol (T) (1 mmol/L, administered in drinking water). Mean arterial pressure (MAP), glomerular filtration rate (GFR), and urinary sodium excretion (UVNa) were measured. We evaluated angiotensin II (Ang II), angiotensin 1-7 (Ang 1-7), angiotensin converting enzyme 2 (ACE2), mas receptor (MasR), angiotensin type 1 receptor (AT1R) and angiotensin type 2 receptor (AT2R) in renal tissues by immunohistochemistry. RESULTS: The intake of high sodium produced a slight but significant increase in MAP and differentially regulated components of the renal renin-angiotensin system (RAS). This included an increase in Ang II and AT1R, and decrease in ACE-2 staining intensity using immunohistochemistry. Antioxidant supplementation with tempol increased natriuresis and GFR, prevented changes in blood pressure and reversed the imbalance of renal RAS components. This includes a decrease in Ang II and AT1R, as increase in AT2, ACE2, Ang (1-7) and MasR staining intensity using immunohistochemistry. In addition, the natriuretic effects of tempol were observed in NS-T group, which showed an increased staining intensity of AT2, ACE2, Ang (1-7) and MasR. CONCLUSION: These findings suggest that a high salt diet leads to changes in the homeostasis and balance between opposing components of the renal RAS in hypertension to favour an increase in Ang II. Chronic antioxidant supplementation can modulate the balance between the natriuretic and antinatriuretic components of the renal RAS. Baishideng Publishing Group Inc 2017-01-06 2017-01-06 /pmc/articles/PMC5215206/ /pubmed/28101449 http://dx.doi.org/10.5527/wjn.v6.i1.29 Text en ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. |
spellingShingle | Basic Study Cao, Gabriel Della Penna, Silvana Lorena Kouyoumdzian, Nicolás Martín Choi, Marcelo Roberto Gorzalczany, Susana Fernández, Belisario Enrique Toblli, Jorge Eduardo Rosón, María Inés Immunohistochemical expression of intrarenal renin angiotensin system components in response to tempol in rats fed a high salt diet |
title | Immunohistochemical expression of intrarenal renin angiotensin system components in response to tempol in rats fed a high salt diet |
title_full | Immunohistochemical expression of intrarenal renin angiotensin system components in response to tempol in rats fed a high salt diet |
title_fullStr | Immunohistochemical expression of intrarenal renin angiotensin system components in response to tempol in rats fed a high salt diet |
title_full_unstemmed | Immunohistochemical expression of intrarenal renin angiotensin system components in response to tempol in rats fed a high salt diet |
title_short | Immunohistochemical expression of intrarenal renin angiotensin system components in response to tempol in rats fed a high salt diet |
title_sort | immunohistochemical expression of intrarenal renin angiotensin system components in response to tempol in rats fed a high salt diet |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215206/ https://www.ncbi.nlm.nih.gov/pubmed/28101449 http://dx.doi.org/10.5527/wjn.v6.i1.29 |
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