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High throughput toxicity screening and intracellular detection of nanomaterials

With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety—preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assess...

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Autores principales: Collins, Andrew R., Annangi, Balasubramanyam, Rubio, Laura, Marcos, Ricard, Dorn, Marco, Merker, Carolin, Estrela‐Lopis, Irina, Cimpan, Mihaela Roxana, Ibrahim, Mohamed, Cimpan, Emil, Ostermann, Melanie, Sauter, Alexander, Yamani, Naouale El, Shaposhnikov, Sergey, Chevillard, Sylvie, Paget, Vincent, Grall, Romain, Delic, Jozo, de‐Cerio, Felipe Goñi‐, Suarez‐Merino, Blanca, Fessard, Valérie, Hogeveen, Kevin N., Fjellsbø, Lise Maria, Pran, Elise Runden, Brzicova, Tana, Topinka, Jan, Silva, Maria João, Leite, P. E., Ribeiro, A. R., Granjeiro, J. M., Grafström, Roland, Prina‐Mello, Adriele, Dusinska, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215403/
https://www.ncbi.nlm.nih.gov/pubmed/27273980
http://dx.doi.org/10.1002/wnan.1413
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author Collins, Andrew R.
Annangi, Balasubramanyam
Rubio, Laura
Marcos, Ricard
Dorn, Marco
Merker, Carolin
Estrela‐Lopis, Irina
Cimpan, Mihaela Roxana
Ibrahim, Mohamed
Cimpan, Emil
Ostermann, Melanie
Sauter, Alexander
Yamani, Naouale El
Shaposhnikov, Sergey
Chevillard, Sylvie
Paget, Vincent
Grall, Romain
Delic, Jozo
de‐Cerio, Felipe Goñi‐
Suarez‐Merino, Blanca
Fessard, Valérie
Hogeveen, Kevin N.
Fjellsbø, Lise Maria
Pran, Elise Runden
Brzicova, Tana
Topinka, Jan
Silva, Maria João
Leite, P. E.
Ribeiro, A. R.
Granjeiro, J. M.
Grafström, Roland
Prina‐Mello, Adriele
Dusinska, Maria
author_facet Collins, Andrew R.
Annangi, Balasubramanyam
Rubio, Laura
Marcos, Ricard
Dorn, Marco
Merker, Carolin
Estrela‐Lopis, Irina
Cimpan, Mihaela Roxana
Ibrahim, Mohamed
Cimpan, Emil
Ostermann, Melanie
Sauter, Alexander
Yamani, Naouale El
Shaposhnikov, Sergey
Chevillard, Sylvie
Paget, Vincent
Grall, Romain
Delic, Jozo
de‐Cerio, Felipe Goñi‐
Suarez‐Merino, Blanca
Fessard, Valérie
Hogeveen, Kevin N.
Fjellsbø, Lise Maria
Pran, Elise Runden
Brzicova, Tana
Topinka, Jan
Silva, Maria João
Leite, P. E.
Ribeiro, A. R.
Granjeiro, J. M.
Grafström, Roland
Prina‐Mello, Adriele
Dusinska, Maria
author_sort Collins, Andrew R.
collection PubMed
description With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety—preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read‐across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter‐experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM‐cell interactions. Validation of in vitro HTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose‐ and time‐dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label‐free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance‐based monitoring, Multiplex analysis of secreted products, and genotoxicity methods—namely High throughput comet assay, High throughput in vitro micronucleus assay, and γH2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test; possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed. WIREs Nanomed Nanobiotechnol 2017, 9:e1413. doi: 10.1002/wnan.1413 For further resources related to this article, please visit the WIREs website.
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spelling pubmed-52154032017-01-18 High throughput toxicity screening and intracellular detection of nanomaterials Collins, Andrew R. Annangi, Balasubramanyam Rubio, Laura Marcos, Ricard Dorn, Marco Merker, Carolin Estrela‐Lopis, Irina Cimpan, Mihaela Roxana Ibrahim, Mohamed Cimpan, Emil Ostermann, Melanie Sauter, Alexander Yamani, Naouale El Shaposhnikov, Sergey Chevillard, Sylvie Paget, Vincent Grall, Romain Delic, Jozo de‐Cerio, Felipe Goñi‐ Suarez‐Merino, Blanca Fessard, Valérie Hogeveen, Kevin N. Fjellsbø, Lise Maria Pran, Elise Runden Brzicova, Tana Topinka, Jan Silva, Maria João Leite, P. E. Ribeiro, A. R. Granjeiro, J. M. Grafström, Roland Prina‐Mello, Adriele Dusinska, Maria Wiley Interdiscip Rev Nanomed Nanobiotechnol Advanced Reviews With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety—preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read‐across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter‐experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM‐cell interactions. Validation of in vitro HTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose‐ and time‐dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label‐free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance‐based monitoring, Multiplex analysis of secreted products, and genotoxicity methods—namely High throughput comet assay, High throughput in vitro micronucleus assay, and γH2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test; possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed. WIREs Nanomed Nanobiotechnol 2017, 9:e1413. doi: 10.1002/wnan.1413 For further resources related to this article, please visit the WIREs website. John Wiley & Sons, Inc. 2016-06-07 2017 /pmc/articles/PMC5215403/ /pubmed/27273980 http://dx.doi.org/10.1002/wnan.1413 Text en © 2016 The Authors. WIREs Nanomedicine and Nanobiotechnology published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Advanced Reviews
Collins, Andrew R.
Annangi, Balasubramanyam
Rubio, Laura
Marcos, Ricard
Dorn, Marco
Merker, Carolin
Estrela‐Lopis, Irina
Cimpan, Mihaela Roxana
Ibrahim, Mohamed
Cimpan, Emil
Ostermann, Melanie
Sauter, Alexander
Yamani, Naouale El
Shaposhnikov, Sergey
Chevillard, Sylvie
Paget, Vincent
Grall, Romain
Delic, Jozo
de‐Cerio, Felipe Goñi‐
Suarez‐Merino, Blanca
Fessard, Valérie
Hogeveen, Kevin N.
Fjellsbø, Lise Maria
Pran, Elise Runden
Brzicova, Tana
Topinka, Jan
Silva, Maria João
Leite, P. E.
Ribeiro, A. R.
Granjeiro, J. M.
Grafström, Roland
Prina‐Mello, Adriele
Dusinska, Maria
High throughput toxicity screening and intracellular detection of nanomaterials
title High throughput toxicity screening and intracellular detection of nanomaterials
title_full High throughput toxicity screening and intracellular detection of nanomaterials
title_fullStr High throughput toxicity screening and intracellular detection of nanomaterials
title_full_unstemmed High throughput toxicity screening and intracellular detection of nanomaterials
title_short High throughput toxicity screening and intracellular detection of nanomaterials
title_sort high throughput toxicity screening and intracellular detection of nanomaterials
topic Advanced Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215403/
https://www.ncbi.nlm.nih.gov/pubmed/27273980
http://dx.doi.org/10.1002/wnan.1413
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