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Maternal exposure to a Western‐style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups

SCOPE: The long‐lasting consequences of nutritional programming during the early phase of life have become increasingly evident. The effects of maternal nutrition on the developing intestine are still underexplored. METHODS AND RESULTS: In this study, we observed (1) altered microbiota composition o...

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Autores principales: Steegenga, Wilma T., Mischke, Mona, Lute, Carolien, Boekschoten, Mark V., Lendvai, Agnes, Pruis, Maurien G. M., Verkade, Henkjan J., van de Heijning, Bert J. M., Boekhorst, Jos, Timmerman, Harro M., Plösch, Torsten, Müller, Michael, Hooiveld, Guido J. E. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215441/
https://www.ncbi.nlm.nih.gov/pubmed/27129739
http://dx.doi.org/10.1002/mnfr.201600141
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author Steegenga, Wilma T.
Mischke, Mona
Lute, Carolien
Boekschoten, Mark V.
Lendvai, Agnes
Pruis, Maurien G. M.
Verkade, Henkjan J.
van de Heijning, Bert J. M.
Boekhorst, Jos
Timmerman, Harro M.
Plösch, Torsten
Müller, Michael
Hooiveld, Guido J. E. J.
author_facet Steegenga, Wilma T.
Mischke, Mona
Lute, Carolien
Boekschoten, Mark V.
Lendvai, Agnes
Pruis, Maurien G. M.
Verkade, Henkjan J.
van de Heijning, Bert J. M.
Boekhorst, Jos
Timmerman, Harro M.
Plösch, Torsten
Müller, Michael
Hooiveld, Guido J. E. J.
author_sort Steegenga, Wilma T.
collection PubMed
description SCOPE: The long‐lasting consequences of nutritional programming during the early phase of life have become increasingly evident. The effects of maternal nutrition on the developing intestine are still underexplored. METHODS AND RESULTS: In this study, we observed (1) altered microbiota composition of the colonic luminal content, and (2) differential gene expression in the intestinal wall in 2‐week‐old mouse pups born from dams exposed to a Western‐style (WS) diet during the perinatal period. A sexually dimorphic effect was found for the differentially expressed genes in the offspring of WS diet‐exposed dams but no differences between male and female pups were found for the microbiota composition. Integrative analysis of the microbiota and gene expression data revealed that the maternal WS diet independently affected gene expression and microbiota composition. However, the abundance of bacterial families not affected by the WS diet (Bacteroidaceae, Porphyromonadaceae, and Lachnospiraceae) correlated with the expression of genes playing a key role in intestinal development and functioning (e.g. Pitx2 and Ace2). CONCLUSION: Our data reveal that maternal consumption of a WS diet during the perinatal period alters both gene expression and microbiota composition in the intestinal tract of 2‐week‐old offspring.
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spelling pubmed-52154412017-01-18 Maternal exposure to a Western‐style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups Steegenga, Wilma T. Mischke, Mona Lute, Carolien Boekschoten, Mark V. Lendvai, Agnes Pruis, Maurien G. M. Verkade, Henkjan J. van de Heijning, Bert J. M. Boekhorst, Jos Timmerman, Harro M. Plösch, Torsten Müller, Michael Hooiveld, Guido J. E. J. Mol Nutr Food Res Research Articles SCOPE: The long‐lasting consequences of nutritional programming during the early phase of life have become increasingly evident. The effects of maternal nutrition on the developing intestine are still underexplored. METHODS AND RESULTS: In this study, we observed (1) altered microbiota composition of the colonic luminal content, and (2) differential gene expression in the intestinal wall in 2‐week‐old mouse pups born from dams exposed to a Western‐style (WS) diet during the perinatal period. A sexually dimorphic effect was found for the differentially expressed genes in the offspring of WS diet‐exposed dams but no differences between male and female pups were found for the microbiota composition. Integrative analysis of the microbiota and gene expression data revealed that the maternal WS diet independently affected gene expression and microbiota composition. However, the abundance of bacterial families not affected by the WS diet (Bacteroidaceae, Porphyromonadaceae, and Lachnospiraceae) correlated with the expression of genes playing a key role in intestinal development and functioning (e.g. Pitx2 and Ace2). CONCLUSION: Our data reveal that maternal consumption of a WS diet during the perinatal period alters both gene expression and microbiota composition in the intestinal tract of 2‐week‐old offspring. John Wiley and Sons Inc. 2016-07-12 2017-01 /pmc/articles/PMC5215441/ /pubmed/27129739 http://dx.doi.org/10.1002/mnfr.201600141 Text en © 2016 The Authors. Molecular Nutrition & Food Research Published by Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Steegenga, Wilma T.
Mischke, Mona
Lute, Carolien
Boekschoten, Mark V.
Lendvai, Agnes
Pruis, Maurien G. M.
Verkade, Henkjan J.
van de Heijning, Bert J. M.
Boekhorst, Jos
Timmerman, Harro M.
Plösch, Torsten
Müller, Michael
Hooiveld, Guido J. E. J.
Maternal exposure to a Western‐style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups
title Maternal exposure to a Western‐style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups
title_full Maternal exposure to a Western‐style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups
title_fullStr Maternal exposure to a Western‐style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups
title_full_unstemmed Maternal exposure to a Western‐style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups
title_short Maternal exposure to a Western‐style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups
title_sort maternal exposure to a western‐style diet causes differences in intestinal microbiota composition and gene expression of suckling mouse pups
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215441/
https://www.ncbi.nlm.nih.gov/pubmed/27129739
http://dx.doi.org/10.1002/mnfr.201600141
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