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Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona
Protein corona of nanoparticles (NPs), which forms when these particles come in to contact with protein-containing fluids, is considered as an overlooked factor in nanomedicine. Through numerous studies it has been becoming increasingly evident that it importantly dictates the interaction of NPs wit...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215476/ https://www.ncbi.nlm.nih.gov/pubmed/28052135 http://dx.doi.org/10.1371/journal.pone.0169552 |
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author | Strojan, Klemen Leonardi, Adrijana Bregar, Vladimir B. Križaj, Igor Svete, Jurij Pavlin, Mojca |
author_facet | Strojan, Klemen Leonardi, Adrijana Bregar, Vladimir B. Križaj, Igor Svete, Jurij Pavlin, Mojca |
author_sort | Strojan, Klemen |
collection | PubMed |
description | Protein corona of nanoparticles (NPs), which forms when these particles come in to contact with protein-containing fluids, is considered as an overlooked factor in nanomedicine. Through numerous studies it has been becoming increasingly evident that it importantly dictates the interaction of NPs with their surroundings. Several factors that determine the compositions of NPs protein corona have been identified in recent years, but one has remained largely ignored—the composition of media used for dispersion of NPs. Here, we determined the effect of dispersion media on the composition of protein corona of polyacrylic acid-coated cobalt ferrite NPs (PAA NPs) and silica NPs. Our results confirmed some of the basic premises such as NPs type-dependent specificity of the protein corona. But more importantly, we demonstrated the effect of the dispersion media on the protein corona composition. The differences between constituents of the media used for dispersion of NPs, such as divalent ions and macromolecules were responsible for the differences in protein corona composition formed in the presence of fetal bovine serum (FBS). Our results suggest that the protein corona composition is a complex function of the constituents present in the media used for dispersion of NPs. Regardless of the dispersion media and FBS concentration, majority of proteins from either PAA NPs or silica NPs coronas were involved in the process of transport and hemostasis. Interestingly, corona of silica NPs contained three complement system related proteins: complement factor H, complement C3 and complement C4 while PAA NPs bound only one immune system related protein, α-2-glycoprotein. Importantly, relative abundance of complement C3 protein in corona of silica NPs was increased when NPs were dispersed in NaCl, which further implies the relevance of dispersion media used to prepare NPs. |
format | Online Article Text |
id | pubmed-5215476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-52154762017-01-19 Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona Strojan, Klemen Leonardi, Adrijana Bregar, Vladimir B. Križaj, Igor Svete, Jurij Pavlin, Mojca PLoS One Research Article Protein corona of nanoparticles (NPs), which forms when these particles come in to contact with protein-containing fluids, is considered as an overlooked factor in nanomedicine. Through numerous studies it has been becoming increasingly evident that it importantly dictates the interaction of NPs with their surroundings. Several factors that determine the compositions of NPs protein corona have been identified in recent years, but one has remained largely ignored—the composition of media used for dispersion of NPs. Here, we determined the effect of dispersion media on the composition of protein corona of polyacrylic acid-coated cobalt ferrite NPs (PAA NPs) and silica NPs. Our results confirmed some of the basic premises such as NPs type-dependent specificity of the protein corona. But more importantly, we demonstrated the effect of the dispersion media on the protein corona composition. The differences between constituents of the media used for dispersion of NPs, such as divalent ions and macromolecules were responsible for the differences in protein corona composition formed in the presence of fetal bovine serum (FBS). Our results suggest that the protein corona composition is a complex function of the constituents present in the media used for dispersion of NPs. Regardless of the dispersion media and FBS concentration, majority of proteins from either PAA NPs or silica NPs coronas were involved in the process of transport and hemostasis. Interestingly, corona of silica NPs contained three complement system related proteins: complement factor H, complement C3 and complement C4 while PAA NPs bound only one immune system related protein, α-2-glycoprotein. Importantly, relative abundance of complement C3 protein in corona of silica NPs was increased when NPs were dispersed in NaCl, which further implies the relevance of dispersion media used to prepare NPs. Public Library of Science 2017-01-04 /pmc/articles/PMC5215476/ /pubmed/28052135 http://dx.doi.org/10.1371/journal.pone.0169552 Text en © 2017 Strojan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Strojan, Klemen Leonardi, Adrijana Bregar, Vladimir B. Križaj, Igor Svete, Jurij Pavlin, Mojca Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona |
title | Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona |
title_full | Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona |
title_fullStr | Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona |
title_full_unstemmed | Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona |
title_short | Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona |
title_sort | dispersion of nanoparticles in different media importantly determines the composition of their protein corona |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215476/ https://www.ncbi.nlm.nih.gov/pubmed/28052135 http://dx.doi.org/10.1371/journal.pone.0169552 |
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