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In silico study on Arabidopsis BAG gene expression in response to environmental stresses

BAG (Bcl-2 athanogene) family proteins are conserved in a wide range of eukaryotes, and they have been proposed to play a crucial role in plant programmed cell death (PCD). During the past decade, with the help of advanced bioinformatics tools, seven homologs of BAG genes have been identified in the...

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Autores principales: Nawkar, Ganesh M., Maibam, Punyakishore, Park, Joung Hun, Woo, Su Gyeong, Kim, Cha Young, Lee, Sang Yeol, Kang, Chang Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216074/
https://www.ncbi.nlm.nih.gov/pubmed/27002965
http://dx.doi.org/10.1007/s00709-016-0961-3
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author Nawkar, Ganesh M.
Maibam, Punyakishore
Park, Joung Hun
Woo, Su Gyeong
Kim, Cha Young
Lee, Sang Yeol
Kang, Chang Ho
author_facet Nawkar, Ganesh M.
Maibam, Punyakishore
Park, Joung Hun
Woo, Su Gyeong
Kim, Cha Young
Lee, Sang Yeol
Kang, Chang Ho
author_sort Nawkar, Ganesh M.
collection PubMed
description BAG (Bcl-2 athanogene) family proteins are conserved in a wide range of eukaryotes, and they have been proposed to play a crucial role in plant programmed cell death (PCD). During the past decade, with the help of advanced bioinformatics tools, seven homologs of BAG genes have been identified in the Arabidopsis genome; these genes are involved in pathogen attack and abiotic stress conditions. In this study, gene expression of Arabidopsis BAG family members under environmental stresses was analyzed using the Botany Array Resource (BAR) expression browser tool and the in silico data were partially confirmed by qRT-PCR analysis for the selected stress- and hormone-treated conditions related to environmental stresses. Particularly, the induction of AtBAG6 gene in response to heat shock was confirmed by using GUS reporter lines. The loss of the AtBAG6 gene resulted into impairment in basal thermotolerance of plant and showed enhanced cell death in response to heat stress. To elucidate the regulatory mechanisms of BAG genes, we analyzed ∼1-kbp promoter regions for the presence of stress-responsive elements. Our transcription profiling finally revealed that the Arabidopsis BAG genes differentially respond to environmental stresses under the control of specifically organized upstream regulatory elements. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00709-016-0961-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-52160742017-01-18 In silico study on Arabidopsis BAG gene expression in response to environmental stresses Nawkar, Ganesh M. Maibam, Punyakishore Park, Joung Hun Woo, Su Gyeong Kim, Cha Young Lee, Sang Yeol Kang, Chang Ho Protoplasma Original Article BAG (Bcl-2 athanogene) family proteins are conserved in a wide range of eukaryotes, and they have been proposed to play a crucial role in plant programmed cell death (PCD). During the past decade, with the help of advanced bioinformatics tools, seven homologs of BAG genes have been identified in the Arabidopsis genome; these genes are involved in pathogen attack and abiotic stress conditions. In this study, gene expression of Arabidopsis BAG family members under environmental stresses was analyzed using the Botany Array Resource (BAR) expression browser tool and the in silico data were partially confirmed by qRT-PCR analysis for the selected stress- and hormone-treated conditions related to environmental stresses. Particularly, the induction of AtBAG6 gene in response to heat shock was confirmed by using GUS reporter lines. The loss of the AtBAG6 gene resulted into impairment in basal thermotolerance of plant and showed enhanced cell death in response to heat stress. To elucidate the regulatory mechanisms of BAG genes, we analyzed ∼1-kbp promoter regions for the presence of stress-responsive elements. Our transcription profiling finally revealed that the Arabidopsis BAG genes differentially respond to environmental stresses under the control of specifically organized upstream regulatory elements. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00709-016-0961-3) contains supplementary material, which is available to authorized users. Springer Vienna 2016-03-22 2017 /pmc/articles/PMC5216074/ /pubmed/27002965 http://dx.doi.org/10.1007/s00709-016-0961-3 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Nawkar, Ganesh M.
Maibam, Punyakishore
Park, Joung Hun
Woo, Su Gyeong
Kim, Cha Young
Lee, Sang Yeol
Kang, Chang Ho
In silico study on Arabidopsis BAG gene expression in response to environmental stresses
title In silico study on Arabidopsis BAG gene expression in response to environmental stresses
title_full In silico study on Arabidopsis BAG gene expression in response to environmental stresses
title_fullStr In silico study on Arabidopsis BAG gene expression in response to environmental stresses
title_full_unstemmed In silico study on Arabidopsis BAG gene expression in response to environmental stresses
title_short In silico study on Arabidopsis BAG gene expression in response to environmental stresses
title_sort in silico study on arabidopsis bag gene expression in response to environmental stresses
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216074/
https://www.ncbi.nlm.nih.gov/pubmed/27002965
http://dx.doi.org/10.1007/s00709-016-0961-3
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