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A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer

Multiple pregnancy is a risk for prematurity and preterm birth. The goal of assisted reproduction is to achieve a single pregnancy, by transferring a single embryo. This requires improved methods to identify the competent embryo. Here, we describe such a test, based on flow cytometric determination...

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Autores principales: Pallinger, Eva, Bognar, Zoltan, Bodis, Jozsef, Csabai, Timea, Farkas, Nelli, Godony, Krisztina, Varnagy, Akos, Buzas, Edit, Szekeres-Bartho, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216337/
https://www.ncbi.nlm.nih.gov/pubmed/28057937
http://dx.doi.org/10.1038/srep39927
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author Pallinger, Eva
Bognar, Zoltan
Bodis, Jozsef
Csabai, Timea
Farkas, Nelli
Godony, Krisztina
Varnagy, Akos
Buzas, Edit
Szekeres-Bartho, Julia
author_facet Pallinger, Eva
Bognar, Zoltan
Bodis, Jozsef
Csabai, Timea
Farkas, Nelli
Godony, Krisztina
Varnagy, Akos
Buzas, Edit
Szekeres-Bartho, Julia
author_sort Pallinger, Eva
collection PubMed
description Multiple pregnancy is a risk for prematurity and preterm birth. The goal of assisted reproduction is to achieve a single pregnancy, by transferring a single embryo. This requires improved methods to identify the competent embryo. Here, we describe such a test, based on flow cytometric determination of the nucleic acid (PI+) containing extracellular vesicle (EV) count in day 5 embryo culture media. 88 women undergoing IVF were included in the study. More than 1 embryos were transferred to most patients. In 58 women, the transfer resulted in clinical pregnancy, whereas in 30 women in implantation failure. In 112 culture media of embryos from the “clinical pregnancy” group, the number of PI+ EVs was significantly lower than in those of 49 embryos, from the “implantation failure” group. In 14 women, transfer of a single embryo resulted in a singleton pregnancy, or, transfer of two embryos in twin pregnancy. The culture media of 19 out of the 20 “confirmed competent” embryos contained a lower level of PI+ EVs than the cut off level, suggesting that the competent embryo can indeed be identified by low PI+ EV counts. We developed a noninvasive, simple, inexpensive, quick test, which identifies the embryos that are most likely to implant.
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spelling pubmed-52163372017-01-09 A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer Pallinger, Eva Bognar, Zoltan Bodis, Jozsef Csabai, Timea Farkas, Nelli Godony, Krisztina Varnagy, Akos Buzas, Edit Szekeres-Bartho, Julia Sci Rep Article Multiple pregnancy is a risk for prematurity and preterm birth. The goal of assisted reproduction is to achieve a single pregnancy, by transferring a single embryo. This requires improved methods to identify the competent embryo. Here, we describe such a test, based on flow cytometric determination of the nucleic acid (PI+) containing extracellular vesicle (EV) count in day 5 embryo culture media. 88 women undergoing IVF were included in the study. More than 1 embryos were transferred to most patients. In 58 women, the transfer resulted in clinical pregnancy, whereas in 30 women in implantation failure. In 112 culture media of embryos from the “clinical pregnancy” group, the number of PI+ EVs was significantly lower than in those of 49 embryos, from the “implantation failure” group. In 14 women, transfer of a single embryo resulted in a singleton pregnancy, or, transfer of two embryos in twin pregnancy. The culture media of 19 out of the 20 “confirmed competent” embryos contained a lower level of PI+ EVs than the cut off level, suggesting that the competent embryo can indeed be identified by low PI+ EV counts. We developed a noninvasive, simple, inexpensive, quick test, which identifies the embryos that are most likely to implant. Nature Publishing Group 2017-01-06 /pmc/articles/PMC5216337/ /pubmed/28057937 http://dx.doi.org/10.1038/srep39927 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Pallinger, Eva
Bognar, Zoltan
Bodis, Jozsef
Csabai, Timea
Farkas, Nelli
Godony, Krisztina
Varnagy, Akos
Buzas, Edit
Szekeres-Bartho, Julia
A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer
title A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer
title_full A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer
title_fullStr A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer
title_full_unstemmed A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer
title_short A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer
title_sort simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216337/
https://www.ncbi.nlm.nih.gov/pubmed/28057937
http://dx.doi.org/10.1038/srep39927
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