Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting

Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables...

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Detalles Bibliográficos
Autores principales: Atkin-Smith, Georgia K., Paone, Stephanie, Zanker, Damien J., Duan, Mubing, Phan, Than K., Chen, Weisan, Hulett, Mark D., Poon, Ivan K. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216387/
https://www.ncbi.nlm.nih.gov/pubmed/28057919
http://dx.doi.org/10.1038/srep39846
Descripción
Sumario:Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.

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