Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting

Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables...

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Autores principales: Atkin-Smith, Georgia K., Paone, Stephanie, Zanker, Damien J., Duan, Mubing, Phan, Than K., Chen, Weisan, Hulett, Mark D., Poon, Ivan K. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216387/
https://www.ncbi.nlm.nih.gov/pubmed/28057919
http://dx.doi.org/10.1038/srep39846
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author Atkin-Smith, Georgia K.
Paone, Stephanie
Zanker, Damien J.
Duan, Mubing
Phan, Than K.
Chen, Weisan
Hulett, Mark D.
Poon, Ivan K. H.
author_facet Atkin-Smith, Georgia K.
Paone, Stephanie
Zanker, Damien J.
Duan, Mubing
Phan, Than K.
Chen, Weisan
Hulett, Mark D.
Poon, Ivan K. H.
author_sort Atkin-Smith, Georgia K.
collection PubMed
description Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.
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spelling pubmed-52163872017-01-09 Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting Atkin-Smith, Georgia K. Paone, Stephanie Zanker, Damien J. Duan, Mubing Phan, Than K. Chen, Weisan Hulett, Mark D. Poon, Ivan K. H. Sci Rep Article Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples. Nature Publishing Group 2017-01-06 /pmc/articles/PMC5216387/ /pubmed/28057919 http://dx.doi.org/10.1038/srep39846 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Atkin-Smith, Georgia K.
Paone, Stephanie
Zanker, Damien J.
Duan, Mubing
Phan, Than K.
Chen, Weisan
Hulett, Mark D.
Poon, Ivan K. H.
Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting
title Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting
title_full Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting
title_fullStr Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting
title_full_unstemmed Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting
title_short Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting
title_sort isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216387/
https://www.ncbi.nlm.nih.gov/pubmed/28057919
http://dx.doi.org/10.1038/srep39846
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