Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics

Optogenetics, a widely used technique in neuroscience research, is often limited by its invasive nature of application. Here, we present a noninvasive, ultrasound-based technique to introduce optogenetic channels into the brain by temporarily opening the blood-brain barrier (BBB). We demonstrate the...

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Autores principales: Wang, Shutao, Kugelman, Tara, Buch, Amanda, Herman, Mathieu, Han, Yang, Karakatsani, Maria Eleni, Hussaini, S. Abid, Duff, Karen, Konofagou, Elisa E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216389/
https://www.ncbi.nlm.nih.gov/pubmed/28059117
http://dx.doi.org/10.1038/srep39955
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author Wang, Shutao
Kugelman, Tara
Buch, Amanda
Herman, Mathieu
Han, Yang
Karakatsani, Maria Eleni
Hussaini, S. Abid
Duff, Karen
Konofagou, Elisa E.
author_facet Wang, Shutao
Kugelman, Tara
Buch, Amanda
Herman, Mathieu
Han, Yang
Karakatsani, Maria Eleni
Hussaini, S. Abid
Duff, Karen
Konofagou, Elisa E.
author_sort Wang, Shutao
collection PubMed
description Optogenetics, a widely used technique in neuroscience research, is often limited by its invasive nature of application. Here, we present a noninvasive, ultrasound-based technique to introduce optogenetic channels into the brain by temporarily opening the blood-brain barrier (BBB). We demonstrate the efficiency of the method developed and evaluate the bioactivity of the non-invasively introduced channelrhodopsin channels by performing stimulation in freely behaving mice.
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spelling pubmed-52163892017-01-09 Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics Wang, Shutao Kugelman, Tara Buch, Amanda Herman, Mathieu Han, Yang Karakatsani, Maria Eleni Hussaini, S. Abid Duff, Karen Konofagou, Elisa E. Sci Rep Article Optogenetics, a widely used technique in neuroscience research, is often limited by its invasive nature of application. Here, we present a noninvasive, ultrasound-based technique to introduce optogenetic channels into the brain by temporarily opening the blood-brain barrier (BBB). We demonstrate the efficiency of the method developed and evaluate the bioactivity of the non-invasively introduced channelrhodopsin channels by performing stimulation in freely behaving mice. Nature Publishing Group 2017-01-06 /pmc/articles/PMC5216389/ /pubmed/28059117 http://dx.doi.org/10.1038/srep39955 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Shutao
Kugelman, Tara
Buch, Amanda
Herman, Mathieu
Han, Yang
Karakatsani, Maria Eleni
Hussaini, S. Abid
Duff, Karen
Konofagou, Elisa E.
Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics
title Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics
title_full Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics
title_fullStr Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics
title_full_unstemmed Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics
title_short Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics
title_sort non-invasive, focused ultrasound-facilitated gene delivery for optogenetics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216389/
https://www.ncbi.nlm.nih.gov/pubmed/28059117
http://dx.doi.org/10.1038/srep39955
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