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New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag

BACKGROUND: Recombinant protein purification is a crucial step for biochemistry and structural biology fields. Rapid robust purification methods utilize various peptide or protein tags fused to the target protein for affinity purification using corresponding matrices and to enhance solubility. Howev...

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Detalles Bibliográficos
Autores principales:	Biancucci, Marco, Dolores, Jazel S., Wong, Jennifer, Grimshaw, Sarah, Anderson, Wayne F., Satchell, Karla J. F., Kwon, Keehwan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216533/ https://www.ncbi.nlm.nih.gov/pubmed/28056928 http://dx.doi.org/10.1186/s12896-016-0323-4

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