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Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells

BACKGROUND: There remains a great need for effective therapies for cervical cancers, the majority of which are aggressive leaving patients with poor prognosis. METHODS AND RESULTS: Here, we identify a novel candidate therapeutic target, trefoil factor 3 (TFF3) which overexpressed in cervical cancer...

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Autores principales: Yuan, Zhaohu, Chen, Dandan, Chen, Xiaojie, Yang, Huikuan, Wei, Yaming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216547/
https://www.ncbi.nlm.nih.gov/pubmed/28070169
http://dx.doi.org/10.1186/s12935-016-0379-1
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author Yuan, Zhaohu
Chen, Dandan
Chen, Xiaojie
Yang, Huikuan
Wei, Yaming
author_facet Yuan, Zhaohu
Chen, Dandan
Chen, Xiaojie
Yang, Huikuan
Wei, Yaming
author_sort Yuan, Zhaohu
collection PubMed
description BACKGROUND: There remains a great need for effective therapies for cervical cancers, the majority of which are aggressive leaving patients with poor prognosis. METHODS AND RESULTS: Here, we identify a novel candidate therapeutic target, trefoil factor 3 (TFF3) which overexpressed in cervical cancer cells and was associated with reduced postoperative survival. Functional studies demonstrated that TFF3 overexpression promoted the proliferation and invasion of cervical cancer cells, and inhibited the apoptosis by inducing the mRNA changes in SiHa and Hela cell lines. Conversely, TFF3 silencing disrupted the proliferation and invasion of cervical cancer cells, and induced the apoptosis via Click-iT EdU test, flow cytometry analysis and two-dimensional Matrigel Transwell analysis. Western blot analysis showed that overexpression of TFF3 repressed E-cadherin (CDH1) expression to promote the invasion of cervical cancer cells. Furthermore, down-regulated CDH1 via overexpression of TFF3 was significantly up-regulated by virtue of inhibitor of p-STAT3. CONCLUSIONS: These results suggested that TFF3 stimulated the invasion of cervical cancer cells probably by activating the STAT3/CDH1 signaling pathway. Furthermore, overexpression of TFF3 decreased the sensitivity of cervical cancer cells to etoposide by increasing P-glycoprotein (P-gp) functional activity. Overall, our work provides a preclinical proof that TFF3 not only contributes to the malignant progression of cervical cancers and but also is a potential therapeutic target. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12935-016-0379-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-52165472017-01-09 Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells Yuan, Zhaohu Chen, Dandan Chen, Xiaojie Yang, Huikuan Wei, Yaming Cancer Cell Int Primary Research BACKGROUND: There remains a great need for effective therapies for cervical cancers, the majority of which are aggressive leaving patients with poor prognosis. METHODS AND RESULTS: Here, we identify a novel candidate therapeutic target, trefoil factor 3 (TFF3) which overexpressed in cervical cancer cells and was associated with reduced postoperative survival. Functional studies demonstrated that TFF3 overexpression promoted the proliferation and invasion of cervical cancer cells, and inhibited the apoptosis by inducing the mRNA changes in SiHa and Hela cell lines. Conversely, TFF3 silencing disrupted the proliferation and invasion of cervical cancer cells, and induced the apoptosis via Click-iT EdU test, flow cytometry analysis and two-dimensional Matrigel Transwell analysis. Western blot analysis showed that overexpression of TFF3 repressed E-cadherin (CDH1) expression to promote the invasion of cervical cancer cells. Furthermore, down-regulated CDH1 via overexpression of TFF3 was significantly up-regulated by virtue of inhibitor of p-STAT3. CONCLUSIONS: These results suggested that TFF3 stimulated the invasion of cervical cancer cells probably by activating the STAT3/CDH1 signaling pathway. Furthermore, overexpression of TFF3 decreased the sensitivity of cervical cancer cells to etoposide by increasing P-glycoprotein (P-gp) functional activity. Overall, our work provides a preclinical proof that TFF3 not only contributes to the malignant progression of cervical cancers and but also is a potential therapeutic target. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12935-016-0379-1) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-05 /pmc/articles/PMC5216547/ /pubmed/28070169 http://dx.doi.org/10.1186/s12935-016-0379-1 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Primary Research
Yuan, Zhaohu
Chen, Dandan
Chen, Xiaojie
Yang, Huikuan
Wei, Yaming
Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells
title Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells
title_full Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells
title_fullStr Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells
title_full_unstemmed Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells
title_short Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells
title_sort overexpression of trefoil factor 3 (tff3) contributes to the malignant progression in cervical cancer cells
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216547/
https://www.ncbi.nlm.nih.gov/pubmed/28070169
http://dx.doi.org/10.1186/s12935-016-0379-1
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