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Tumor suppressor berberine binds VASP to inhibit cell migration in basal-like breast cancer

Berberine is a plant-derived compound used in traditional Chinese medicine, which has been shown to inhibit cell proliferation and migration in breast cancer. On the other hand, vasodilator-stimulated phosphoprotein (VASP) promotes actin filament elongation and cell migration. We previously showed t...

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Autores principales: Su, Ke, Hu, Pengchao, Wang, Xiaolan, Kuang, Changchun, Xiang, Qingmin, Yang, Fang, Xiang, Jin, Zhu, Shan, Wei, Lei, Zhang, Jingwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216765/
https://www.ncbi.nlm.nih.gov/pubmed/27322681
http://dx.doi.org/10.18632/oncotarget.9968
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author Su, Ke
Hu, Pengchao
Wang, Xiaolan
Kuang, Changchun
Xiang, Qingmin
Yang, Fang
Xiang, Jin
Zhu, Shan
Wei, Lei
Zhang, Jingwei
author_facet Su, Ke
Hu, Pengchao
Wang, Xiaolan
Kuang, Changchun
Xiang, Qingmin
Yang, Fang
Xiang, Jin
Zhu, Shan
Wei, Lei
Zhang, Jingwei
author_sort Su, Ke
collection PubMed
description Berberine is a plant-derived compound used in traditional Chinese medicine, which has been shown to inhibit cell proliferation and migration in breast cancer. On the other hand, vasodilator-stimulated phosphoprotein (VASP) promotes actin filament elongation and cell migration. We previously showed that VASP is overexpressed in high-motility breast cancer cells. Here we investigated whether the anti-tumorigenic effects of berberine are mediated by binding VASP in basal-like breast cancer. Our results show that berberine suppresses proliferation and migration of MDA-MB-231 cells as well as tumor growth in MDA-MB-231 nude mouse xenografts. We also show that berberine binds to VASP, inducing changes in its secondary structure and inhibits actin polymerization. Our study reveals the mechanism underlying berberine's inhibition of cell proliferation and migration in basal-like breast cancer, highlighting the use of berberine as a potential adjuvant therapeutic agent.
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spelling pubmed-52167652017-01-15 Tumor suppressor berberine binds VASP to inhibit cell migration in basal-like breast cancer Su, Ke Hu, Pengchao Wang, Xiaolan Kuang, Changchun Xiang, Qingmin Yang, Fang Xiang, Jin Zhu, Shan Wei, Lei Zhang, Jingwei Oncotarget Research Paper Berberine is a plant-derived compound used in traditional Chinese medicine, which has been shown to inhibit cell proliferation and migration in breast cancer. On the other hand, vasodilator-stimulated phosphoprotein (VASP) promotes actin filament elongation and cell migration. We previously showed that VASP is overexpressed in high-motility breast cancer cells. Here we investigated whether the anti-tumorigenic effects of berberine are mediated by binding VASP in basal-like breast cancer. Our results show that berberine suppresses proliferation and migration of MDA-MB-231 cells as well as tumor growth in MDA-MB-231 nude mouse xenografts. We also show that berberine binds to VASP, inducing changes in its secondary structure and inhibits actin polymerization. Our study reveals the mechanism underlying berberine's inhibition of cell proliferation and migration in basal-like breast cancer, highlighting the use of berberine as a potential adjuvant therapeutic agent. Impact Journals LLC 2016-06-13 /pmc/articles/PMC5216765/ /pubmed/27322681 http://dx.doi.org/10.18632/oncotarget.9968 Text en Copyright: © 2016 Su et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Su, Ke
Hu, Pengchao
Wang, Xiaolan
Kuang, Changchun
Xiang, Qingmin
Yang, Fang
Xiang, Jin
Zhu, Shan
Wei, Lei
Zhang, Jingwei
Tumor suppressor berberine binds VASP to inhibit cell migration in basal-like breast cancer
title Tumor suppressor berberine binds VASP to inhibit cell migration in basal-like breast cancer
title_full Tumor suppressor berberine binds VASP to inhibit cell migration in basal-like breast cancer
title_fullStr Tumor suppressor berberine binds VASP to inhibit cell migration in basal-like breast cancer
title_full_unstemmed Tumor suppressor berberine binds VASP to inhibit cell migration in basal-like breast cancer
title_short Tumor suppressor berberine binds VASP to inhibit cell migration in basal-like breast cancer
title_sort tumor suppressor berberine binds vasp to inhibit cell migration in basal-like breast cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216765/
https://www.ncbi.nlm.nih.gov/pubmed/27322681
http://dx.doi.org/10.18632/oncotarget.9968
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