Disintegrin targeting of an α(v)β(3) integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro

The in vitro efficacy of the disintegrin echistatin was tested on a high-metastatic variant of 143B human osteosarcoma, 143B-LM4, which over-expresses α(v)β(3) integrin. Echistatin is an RGD cyclic peptide and an antagonist of α(v)β(3) integrin. In the present study, echistatin inhibited cell prolif...

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Autores principales: Tome, Yasunori, Kimura, Hiroaki, Kiyuna, Tasuku, Sugimoto, Naotoshi, Tsuchiya, Hiroyuki, Kanaya, Fuminori, Bouvet, Michael, Hoffman, Robert M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216800/
https://www.ncbi.nlm.nih.gov/pubmed/27331872
http://dx.doi.org/10.18632/oncotarget.10111
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author Tome, Yasunori
Kimura, Hiroaki
Kiyuna, Tasuku
Sugimoto, Naotoshi
Tsuchiya, Hiroyuki
Kanaya, Fuminori
Bouvet, Michael
Hoffman, Robert M.
author_facet Tome, Yasunori
Kimura, Hiroaki
Kiyuna, Tasuku
Sugimoto, Naotoshi
Tsuchiya, Hiroyuki
Kanaya, Fuminori
Bouvet, Michael
Hoffman, Robert M.
author_sort Tome, Yasunori
collection PubMed
description The in vitro efficacy of the disintegrin echistatin was tested on a high-metastatic variant of 143B human osteosarcoma, 143B-LM4, which over-expresses α(v)β(3) integrin. Echistatin is an RGD cyclic peptide and an antagonist of α(v)β(3) integrin. In the present study, echistatin inhibited cell proliferation, migration, invasion, and adhesion of 143B-LM4 cells. 143B-LM4 cell proliferation decreased after treatment with echistatin in a time-dependent and dose-dependent manner (P <0.01). In vitro migration and invasion of 143B-LM4 cells were also inhibited by echistatin in a dose-dependent manner (P <0.01, respectively). Cell adhesion to vitronectin of 143B-LM4 cells was also inhibited by echistatin in a dose-dependent manner (P <0.01). These results suggest that α(v)β(3) integrin may be an effective target for osteosarcoma.
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spelling pubmed-52168002017-01-15 Disintegrin targeting of an α(v)β(3) integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro Tome, Yasunori Kimura, Hiroaki Kiyuna, Tasuku Sugimoto, Naotoshi Tsuchiya, Hiroyuki Kanaya, Fuminori Bouvet, Michael Hoffman, Robert M. Oncotarget Research Paper The in vitro efficacy of the disintegrin echistatin was tested on a high-metastatic variant of 143B human osteosarcoma, 143B-LM4, which over-expresses α(v)β(3) integrin. Echistatin is an RGD cyclic peptide and an antagonist of α(v)β(3) integrin. In the present study, echistatin inhibited cell proliferation, migration, invasion, and adhesion of 143B-LM4 cells. 143B-LM4 cell proliferation decreased after treatment with echistatin in a time-dependent and dose-dependent manner (P <0.01). In vitro migration and invasion of 143B-LM4 cells were also inhibited by echistatin in a dose-dependent manner (P <0.01, respectively). Cell adhesion to vitronectin of 143B-LM4 cells was also inhibited by echistatin in a dose-dependent manner (P <0.01). These results suggest that α(v)β(3) integrin may be an effective target for osteosarcoma. Impact Journals LLC 2016-06-16 /pmc/articles/PMC5216800/ /pubmed/27331872 http://dx.doi.org/10.18632/oncotarget.10111 Text en Copyright: © 2016 Tome et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Tome, Yasunori
Kimura, Hiroaki
Kiyuna, Tasuku
Sugimoto, Naotoshi
Tsuchiya, Hiroyuki
Kanaya, Fuminori
Bouvet, Michael
Hoffman, Robert M.
Disintegrin targeting of an α(v)β(3) integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro
title Disintegrin targeting of an α(v)β(3) integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro
title_full Disintegrin targeting of an α(v)β(3) integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro
title_fullStr Disintegrin targeting of an α(v)β(3) integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro
title_full_unstemmed Disintegrin targeting of an α(v)β(3) integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro
title_short Disintegrin targeting of an α(v)β(3) integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro
title_sort disintegrin targeting of an α(v)β(3) integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216800/
https://www.ncbi.nlm.nih.gov/pubmed/27331872
http://dx.doi.org/10.18632/oncotarget.10111
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