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Plasticity of adipose tissue in response to fasting and refeeding in male mice

BACKGROUND: Fasting is the most widely prescribed and self-imposed strategy for treating excessive weight gain and obesity, and has been shown to exert a number of beneficial effects. The aim of the present study was to determine the exact role of fasting and subsequent refeeding on fat distribution...

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Autores principales: Tang, Hao-Neng, Tang, Chen-Yi, Man, Xiao-Fei, Tan, Shu-Wen, Guo, Yue, Tang, Jun, Zhou, Ci-La, Zhou, Hou-De
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217231/
https://www.ncbi.nlm.nih.gov/pubmed/28070205
http://dx.doi.org/10.1186/s12986-016-0159-x
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author Tang, Hao-Neng
Tang, Chen-Yi
Man, Xiao-Fei
Tan, Shu-Wen
Guo, Yue
Tang, Jun
Zhou, Ci-La
Zhou, Hou-De
author_facet Tang, Hao-Neng
Tang, Chen-Yi
Man, Xiao-Fei
Tan, Shu-Wen
Guo, Yue
Tang, Jun
Zhou, Ci-La
Zhou, Hou-De
author_sort Tang, Hao-Neng
collection PubMed
description BACKGROUND: Fasting is the most widely prescribed and self-imposed strategy for treating excessive weight gain and obesity, and has been shown to exert a number of beneficial effects. The aim of the present study was to determine the exact role of fasting and subsequent refeeding on fat distribution in mice. METHODS: C57/BL6 mice fasted for 24 to 72 h and were then subjected to refeeding for 72 h. At 24, 48 and 72 h of fasting, and 12, 24, 48 and 72 h of refeeding, the mice were sacrificed, and serum and various adipose tissues were collected. Serum biochemical parameters, adipose tissue masses and histomorphological analysis of different depots were detected. MRNA was isolated from various adipose tissues, and the expressions of thermogenesis, visceral signature and lipid metabolism-related genes were examined. The phenotypes of adipose tissues between juvenile and adult mice subjected to fasting and refeeding were also compared. RESULTS: Fasting preferentially consumed mesenteric fat mass and decreased the cell size of mesenteric depots; however, refeeding recovered the mass and morphology of inguinal adipose tissues preferentially compared with visceral depots. Thermogenesis-related gene expression in the inguinal WAT and interscapular BAT were suppressed. Mitochondrial biogenesis was affected by fasting in a depot-specific manner. Furthermore, a short period of fasting led to an increase in visceral signature genes (Wt1, Tcf21) in subcutaneous adipose tissue, while the expression of these genes decreased sharply as the fasting time increased. Additionally, lipogenesis-related markers were enhanced to a greater extent greater in subcutaneous depots compared with those in visceral adipose tissues by refeeding. Although similar phenotypic changes in adipose tissue were observed between juvenile mice and adult mice subjected to fasting and refeeding, the alterations appeared earlier and more sensitively in juvenile mice. CONCLUSIONS: Fasting preferentially consumes lipids in visceral adipose tissues, whereas refeeding recovers lipids predominantly in subcutaneous adipose tissues, which indicated the significance of plasticity of adipose organs for fat distribution when subject to food deprivation or refeeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12986-016-0159-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-52172312017-01-09 Plasticity of adipose tissue in response to fasting and refeeding in male mice Tang, Hao-Neng Tang, Chen-Yi Man, Xiao-Fei Tan, Shu-Wen Guo, Yue Tang, Jun Zhou, Ci-La Zhou, Hou-De Nutr Metab (Lond) Research BACKGROUND: Fasting is the most widely prescribed and self-imposed strategy for treating excessive weight gain and obesity, and has been shown to exert a number of beneficial effects. The aim of the present study was to determine the exact role of fasting and subsequent refeeding on fat distribution in mice. METHODS: C57/BL6 mice fasted for 24 to 72 h and were then subjected to refeeding for 72 h. At 24, 48 and 72 h of fasting, and 12, 24, 48 and 72 h of refeeding, the mice were sacrificed, and serum and various adipose tissues were collected. Serum biochemical parameters, adipose tissue masses and histomorphological analysis of different depots were detected. MRNA was isolated from various adipose tissues, and the expressions of thermogenesis, visceral signature and lipid metabolism-related genes were examined. The phenotypes of adipose tissues between juvenile and adult mice subjected to fasting and refeeding were also compared. RESULTS: Fasting preferentially consumed mesenteric fat mass and decreased the cell size of mesenteric depots; however, refeeding recovered the mass and morphology of inguinal adipose tissues preferentially compared with visceral depots. Thermogenesis-related gene expression in the inguinal WAT and interscapular BAT were suppressed. Mitochondrial biogenesis was affected by fasting in a depot-specific manner. Furthermore, a short period of fasting led to an increase in visceral signature genes (Wt1, Tcf21) in subcutaneous adipose tissue, while the expression of these genes decreased sharply as the fasting time increased. Additionally, lipogenesis-related markers were enhanced to a greater extent greater in subcutaneous depots compared with those in visceral adipose tissues by refeeding. Although similar phenotypic changes in adipose tissue were observed between juvenile mice and adult mice subjected to fasting and refeeding, the alterations appeared earlier and more sensitively in juvenile mice. CONCLUSIONS: Fasting preferentially consumes lipids in visceral adipose tissues, whereas refeeding recovers lipids predominantly in subcutaneous adipose tissues, which indicated the significance of plasticity of adipose organs for fat distribution when subject to food deprivation or refeeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12986-016-0159-x) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-05 /pmc/articles/PMC5217231/ /pubmed/28070205 http://dx.doi.org/10.1186/s12986-016-0159-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Tang, Hao-Neng
Tang, Chen-Yi
Man, Xiao-Fei
Tan, Shu-Wen
Guo, Yue
Tang, Jun
Zhou, Ci-La
Zhou, Hou-De
Plasticity of adipose tissue in response to fasting and refeeding in male mice
title Plasticity of adipose tissue in response to fasting and refeeding in male mice
title_full Plasticity of adipose tissue in response to fasting and refeeding in male mice
title_fullStr Plasticity of adipose tissue in response to fasting and refeeding in male mice
title_full_unstemmed Plasticity of adipose tissue in response to fasting and refeeding in male mice
title_short Plasticity of adipose tissue in response to fasting and refeeding in male mice
title_sort plasticity of adipose tissue in response to fasting and refeeding in male mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217231/
https://www.ncbi.nlm.nih.gov/pubmed/28070205
http://dx.doi.org/10.1186/s12986-016-0159-x
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