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Absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using F344 gpt delta rats

INTRODUCTION: It is known that fibrous particles of micrometer length, such as carbon nanotubes, which have same dimensions as asbestos, are carcinogenic. Carcinogenicity of nanomaterials is strongly related to inflammatory reactions; however, the genotoxicity mechanism(s) is unclear. Indeed, incons...

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Autores principales: Horibata, Katsuyoshi, Ukai, Akiko, Ogata, Akio, Nakae, Dai, Ando, Hiroshi, Kubo, Yoshikazu, Nagasawa, Akemichi, Yuzawa, Katsuhiro, Honma, Masamitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217301/
https://www.ncbi.nlm.nih.gov/pubmed/28074111
http://dx.doi.org/10.1186/s41021-016-0065-5
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author Horibata, Katsuyoshi
Ukai, Akiko
Ogata, Akio
Nakae, Dai
Ando, Hiroshi
Kubo, Yoshikazu
Nagasawa, Akemichi
Yuzawa, Katsuhiro
Honma, Masamitsu
author_facet Horibata, Katsuyoshi
Ukai, Akiko
Ogata, Akio
Nakae, Dai
Ando, Hiroshi
Kubo, Yoshikazu
Nagasawa, Akemichi
Yuzawa, Katsuhiro
Honma, Masamitsu
author_sort Horibata, Katsuyoshi
collection PubMed
description INTRODUCTION: It is known that fibrous particles of micrometer length, such as carbon nanotubes, which have same dimensions as asbestos, are carcinogenic. Carcinogenicity of nanomaterials is strongly related to inflammatory reactions; however, the genotoxicity mechanism(s) is unclear. Indeed, inconsistent results on genotoxicity of multi-walled carbon nanotubes (MWCNTs) have been shown in several reports. Therefore, we analyzed the in vivo genotoxicity induced by an intratracheal instillation of straight MWCNTs in rats using a different test system—the Pig-a gene mutation assay—that can reflect the genotoxicity occurring in the bone marrow. Since lungs were directly exposed to MWCNTs upon intratracheal instillation, we also performed the gpt assay using the lungs. FINDINGS: We detected no significant differences in Pig-a mutant frequencies (MFs) between the MWCNT-treated and control rats. Additionally, we detected no significant differences in gpt MFs in the lung between the MWCNT-treated and control rats. CONCLUSIONS: Our findings indicated that a single intratracheal instillation of MWCNTs was non-mutagenic to both the bone marrow and lung of rats.
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spelling pubmed-52173012017-01-10 Absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using F344 gpt delta rats Horibata, Katsuyoshi Ukai, Akiko Ogata, Akio Nakae, Dai Ando, Hiroshi Kubo, Yoshikazu Nagasawa, Akemichi Yuzawa, Katsuhiro Honma, Masamitsu Genes Environ Short Report INTRODUCTION: It is known that fibrous particles of micrometer length, such as carbon nanotubes, which have same dimensions as asbestos, are carcinogenic. Carcinogenicity of nanomaterials is strongly related to inflammatory reactions; however, the genotoxicity mechanism(s) is unclear. Indeed, inconsistent results on genotoxicity of multi-walled carbon nanotubes (MWCNTs) have been shown in several reports. Therefore, we analyzed the in vivo genotoxicity induced by an intratracheal instillation of straight MWCNTs in rats using a different test system—the Pig-a gene mutation assay—that can reflect the genotoxicity occurring in the bone marrow. Since lungs were directly exposed to MWCNTs upon intratracheal instillation, we also performed the gpt assay using the lungs. FINDINGS: We detected no significant differences in Pig-a mutant frequencies (MFs) between the MWCNT-treated and control rats. Additionally, we detected no significant differences in gpt MFs in the lung between the MWCNT-treated and control rats. CONCLUSIONS: Our findings indicated that a single intratracheal instillation of MWCNTs was non-mutagenic to both the bone marrow and lung of rats. BioMed Central 2017-01-06 /pmc/articles/PMC5217301/ /pubmed/28074111 http://dx.doi.org/10.1186/s41021-016-0065-5 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Horibata, Katsuyoshi
Ukai, Akiko
Ogata, Akio
Nakae, Dai
Ando, Hiroshi
Kubo, Yoshikazu
Nagasawa, Akemichi
Yuzawa, Katsuhiro
Honma, Masamitsu
Absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using F344 gpt delta rats
title Absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using F344 gpt delta rats
title_full Absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using F344 gpt delta rats
title_fullStr Absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using F344 gpt delta rats
title_full_unstemmed Absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using F344 gpt delta rats
title_short Absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using F344 gpt delta rats
title_sort absence of in vivo mutagenicity of multi-walled carbon nanotubes in single intratracheal instillation study using f344 gpt delta rats
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217301/
https://www.ncbi.nlm.nih.gov/pubmed/28074111
http://dx.doi.org/10.1186/s41021-016-0065-5
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