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Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence
In 2013, several Austrian piglet-producing farms recorded outbreaks of action-related repetitive myoclonia in newborn piglets (“shaking piglets”). Malnutrition was seen in numerous piglets as a complication of this tremor syndrome. Overall piglet mortality was increased and the number of weaned pigl...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217315/ https://www.ncbi.nlm.nih.gov/pubmed/28057061 http://dx.doi.org/10.1186/s13567-016-0406-1 |
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author | Schwarz, Lukas Riedel, Christiane Högler, Sandra Sinn, Leonie J. Voglmayr, Thomas Wöchtl, Bettina Dinhopl, Nora Rebel-Bauder, Barbara Weissenböck, Herbert Ladinig, Andrea Rümenapf, Till Lamp, Benjamin |
author_facet | Schwarz, Lukas Riedel, Christiane Högler, Sandra Sinn, Leonie J. Voglmayr, Thomas Wöchtl, Bettina Dinhopl, Nora Rebel-Bauder, Barbara Weissenböck, Herbert Ladinig, Andrea Rümenapf, Till Lamp, Benjamin |
author_sort | Schwarz, Lukas |
collection | PubMed |
description | In 2013, several Austrian piglet-producing farms recorded outbreaks of action-related repetitive myoclonia in newborn piglets (“shaking piglets”). Malnutrition was seen in numerous piglets as a complication of this tremor syndrome. Overall piglet mortality was increased and the number of weaned piglets per sow decreased by more than 10% due to this outbreak. Histological examination of the CNS of affected piglets revealed moderate hypomyelination of the white substance in cerebellum and spinal cord. We detected a recently discovered pestivirus, termed atypical porcine pestivirus (APPV) in all these cases by RT-PCR. A genomic sequence and seven partial sequences were determined and revealed a 90% identity to the US APPV sequences and 92% identity to German sequences. In confirmation with previous reports, APPV genomes were identified in different body fluids and tissues including the CNS of diseased piglets. APPV could be isolated from a “shaking piglet”, which was incapable of consuming colostrum, and passaged on different porcine cells at very low titers. To assess the antibody response a blocking ELISA was developed targeting NS3. APPV specific antibodies were identified in sows and in PCR positive piglets affected by congenital tremor (CT). APPV genomes were detected continuously in piglets that gradually recovered from CT, while the antibody titers decreased over a 12-week interval, pointing towards maternally transmitted antibodies. High viral loads were detectable by qRT-PCR in saliva and semen of infected young adults indicating a persistent infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-016-0406-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5217315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-52173152017-01-09 Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence Schwarz, Lukas Riedel, Christiane Högler, Sandra Sinn, Leonie J. Voglmayr, Thomas Wöchtl, Bettina Dinhopl, Nora Rebel-Bauder, Barbara Weissenböck, Herbert Ladinig, Andrea Rümenapf, Till Lamp, Benjamin Vet Res Research Article In 2013, several Austrian piglet-producing farms recorded outbreaks of action-related repetitive myoclonia in newborn piglets (“shaking piglets”). Malnutrition was seen in numerous piglets as a complication of this tremor syndrome. Overall piglet mortality was increased and the number of weaned piglets per sow decreased by more than 10% due to this outbreak. Histological examination of the CNS of affected piglets revealed moderate hypomyelination of the white substance in cerebellum and spinal cord. We detected a recently discovered pestivirus, termed atypical porcine pestivirus (APPV) in all these cases by RT-PCR. A genomic sequence and seven partial sequences were determined and revealed a 90% identity to the US APPV sequences and 92% identity to German sequences. In confirmation with previous reports, APPV genomes were identified in different body fluids and tissues including the CNS of diseased piglets. APPV could be isolated from a “shaking piglet”, which was incapable of consuming colostrum, and passaged on different porcine cells at very low titers. To assess the antibody response a blocking ELISA was developed targeting NS3. APPV specific antibodies were identified in sows and in PCR positive piglets affected by congenital tremor (CT). APPV genomes were detected continuously in piglets that gradually recovered from CT, while the antibody titers decreased over a 12-week interval, pointing towards maternally transmitted antibodies. High viral loads were detectable by qRT-PCR in saliva and semen of infected young adults indicating a persistent infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-016-0406-1) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-06 2017 /pmc/articles/PMC5217315/ /pubmed/28057061 http://dx.doi.org/10.1186/s13567-016-0406-1 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Schwarz, Lukas Riedel, Christiane Högler, Sandra Sinn, Leonie J. Voglmayr, Thomas Wöchtl, Bettina Dinhopl, Nora Rebel-Bauder, Barbara Weissenböck, Herbert Ladinig, Andrea Rümenapf, Till Lamp, Benjamin Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence |
title | Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence |
title_full | Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence |
title_fullStr | Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence |
title_full_unstemmed | Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence |
title_short | Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence |
title_sort | congenital infection with atypical porcine pestivirus (appv) is associated with disease and viral persistence |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217315/ https://www.ncbi.nlm.nih.gov/pubmed/28057061 http://dx.doi.org/10.1186/s13567-016-0406-1 |
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