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A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants

Clavibacter michiganensis, subsp. michiganensis is a Gram-positive plant pathogen infecting tomato (Solanum lycopersicum). Despite a considerable economic importance due to significant losses of infected plants and fruits, knowledge about virulence factors of C. michiganensis subsp. michiganensis an...

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Autores principales: Hiery, Eva, Poetsch, Ansgar, Moosbauer, Tanja, Amin, Bushra, Hofmann, Jörg, Burkovski, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217389/
https://www.ncbi.nlm.nih.gov/pubmed/28248277
http://dx.doi.org/10.3390/proteomes3040411
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author Hiery, Eva
Poetsch, Ansgar
Moosbauer, Tanja
Amin, Bushra
Hofmann, Jörg
Burkovski, Andreas
author_facet Hiery, Eva
Poetsch, Ansgar
Moosbauer, Tanja
Amin, Bushra
Hofmann, Jörg
Burkovski, Andreas
author_sort Hiery, Eva
collection PubMed
description Clavibacter michiganensis, subsp. michiganensis is a Gram-positive plant pathogen infecting tomato (Solanum lycopersicum). Despite a considerable economic importance due to significant losses of infected plants and fruits, knowledge about virulence factors of C. michiganensis subsp. michiganensis and host-pathogen interactions on a molecular level are rather limited. In the study presented here, the proteome of culture supernatants from C. michiganensis subsp. michiganensis NCPPB382 was analyzed. In total, 1872 proteins were identified in M9 and 1766 proteins in xylem mimicking medium. Filtration of supernatants before protein precipitation reduced these to 1276 proteins in M9 and 976 proteins in the xylem mimicking medium culture filtrate. The results obtained indicate that C. michiganensis subsp. michiganensis reacts to a sucrose- and glucose-depleted medium similar to the xylem sap by utilizing amino acids and host cell polymers as well as their degradation products, mainly peptides, amino acids and various C5 and C6 sugars. Interestingly, the bacterium expresses the previously described virulence factors Pat-1 and CelA not exclusively after host cell contact in planta but already in M9 minimal and xylem mimicking medium.
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spelling pubmed-52173892017-02-27 A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants Hiery, Eva Poetsch, Ansgar Moosbauer, Tanja Amin, Bushra Hofmann, Jörg Burkovski, Andreas Proteomes Article Clavibacter michiganensis, subsp. michiganensis is a Gram-positive plant pathogen infecting tomato (Solanum lycopersicum). Despite a considerable economic importance due to significant losses of infected plants and fruits, knowledge about virulence factors of C. michiganensis subsp. michiganensis and host-pathogen interactions on a molecular level are rather limited. In the study presented here, the proteome of culture supernatants from C. michiganensis subsp. michiganensis NCPPB382 was analyzed. In total, 1872 proteins were identified in M9 and 1766 proteins in xylem mimicking medium. Filtration of supernatants before protein precipitation reduced these to 1276 proteins in M9 and 976 proteins in the xylem mimicking medium culture filtrate. The results obtained indicate that C. michiganensis subsp. michiganensis reacts to a sucrose- and glucose-depleted medium similar to the xylem sap by utilizing amino acids and host cell polymers as well as their degradation products, mainly peptides, amino acids and various C5 and C6 sugars. Interestingly, the bacterium expresses the previously described virulence factors Pat-1 and CelA not exclusively after host cell contact in planta but already in M9 minimal and xylem mimicking medium. MDPI 2015-11-12 /pmc/articles/PMC5217389/ /pubmed/28248277 http://dx.doi.org/10.3390/proteomes3040411 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hiery, Eva
Poetsch, Ansgar
Moosbauer, Tanja
Amin, Bushra
Hofmann, Jörg
Burkovski, Andreas
A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants
title A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants
title_full A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants
title_fullStr A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants
title_full_unstemmed A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants
title_short A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants
title_sort proteomic study of clavibacter michiganensis subsp. michiganensis culture supernatants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217389/
https://www.ncbi.nlm.nih.gov/pubmed/28248277
http://dx.doi.org/10.3390/proteomes3040411
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