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Dynamic relationships between ribosomal conformational and RNA positional changes during ribosomal translocation

Ribosomal translocation catalyzed by EF-G hydrolyzing GTP entails multiple conformational changes of ribosome and positional changes of tRNAs and mRNA in the ribosome. However, the detailed dynamic relations among these changes and EF-G sampling are not clear. Here, based on our proposed pathway of...

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Autor principal: Xie, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5219732/
https://www.ncbi.nlm.nih.gov/pubmed/28070564
http://dx.doi.org/10.1016/j.heliyon.2016.e00214
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author Xie, Ping
author_facet Xie, Ping
author_sort Xie, Ping
collection PubMed
description Ribosomal translocation catalyzed by EF-G hydrolyzing GTP entails multiple conformational changes of ribosome and positional changes of tRNAs and mRNA in the ribosome. However, the detailed dynamic relations among these changes and EF-G sampling are not clear. Here, based on our proposed pathway of ribosomal translocation, we study theoretically the dynamic relations among these changes exhibited in the single molecule data and those exhibited in the ensemble kinetic data. It is shown that the timing of these changes in the single molecule data and that in the ensemble kinetic data show very different. The theoretical results are in agreement with both the available ensemble kinetic experimental data and the single molecule experimental data.
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spelling pubmed-52197322017-01-09 Dynamic relationships between ribosomal conformational and RNA positional changes during ribosomal translocation Xie, Ping Heliyon Article Ribosomal translocation catalyzed by EF-G hydrolyzing GTP entails multiple conformational changes of ribosome and positional changes of tRNAs and mRNA in the ribosome. However, the detailed dynamic relations among these changes and EF-G sampling are not clear. Here, based on our proposed pathway of ribosomal translocation, we study theoretically the dynamic relations among these changes exhibited in the single molecule data and those exhibited in the ensemble kinetic data. It is shown that the timing of these changes in the single molecule data and that in the ensemble kinetic data show very different. The theoretical results are in agreement with both the available ensemble kinetic experimental data and the single molecule experimental data. Elsevier 2016-12-14 /pmc/articles/PMC5219732/ /pubmed/28070564 http://dx.doi.org/10.1016/j.heliyon.2016.e00214 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Xie, Ping
Dynamic relationships between ribosomal conformational and RNA positional changes during ribosomal translocation
title Dynamic relationships between ribosomal conformational and RNA positional changes during ribosomal translocation
title_full Dynamic relationships between ribosomal conformational and RNA positional changes during ribosomal translocation
title_fullStr Dynamic relationships between ribosomal conformational and RNA positional changes during ribosomal translocation
title_full_unstemmed Dynamic relationships between ribosomal conformational and RNA positional changes during ribosomal translocation
title_short Dynamic relationships between ribosomal conformational and RNA positional changes during ribosomal translocation
title_sort dynamic relationships between ribosomal conformational and rna positional changes during ribosomal translocation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5219732/
https://www.ncbi.nlm.nih.gov/pubmed/28070564
http://dx.doi.org/10.1016/j.heliyon.2016.e00214
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