Cargando…

Sex-specific and inter-individual differences in biomarkers of selenium status identified by a calibrated ELISA for selenoprotein P

Selenoprotein P (SELENOP) is a liver-derived transporter of selenium (Se) in blood, and a meaningful biomarker of Se status. Se is an essential trace element for the biosynthesis of enzymatically-active selenoproteins, protecting the organism from oxidative damage. The usage of uncalibrated assays h...

Descripción completa

Detalles Bibliográficos
Autores principales: Hybsier, Sandra, Schulz, Torsten, Wu, Zida, Demuth, Ilja, Minich, Waldemar B., Renko, Kostja, Rijntjes, Eddy, Köhrle, Josef, Strasburger, Christian J., Steinhagen-Thiessen, Elisabeth, Schomburg, Lutz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5220167/
https://www.ncbi.nlm.nih.gov/pubmed/28064116
http://dx.doi.org/10.1016/j.redox.2016.12.025
_version_ 1782492576458211328
author Hybsier, Sandra
Schulz, Torsten
Wu, Zida
Demuth, Ilja
Minich, Waldemar B.
Renko, Kostja
Rijntjes, Eddy
Köhrle, Josef
Strasburger, Christian J.
Steinhagen-Thiessen, Elisabeth
Schomburg, Lutz
author_facet Hybsier, Sandra
Schulz, Torsten
Wu, Zida
Demuth, Ilja
Minich, Waldemar B.
Renko, Kostja
Rijntjes, Eddy
Köhrle, Josef
Strasburger, Christian J.
Steinhagen-Thiessen, Elisabeth
Schomburg, Lutz
author_sort Hybsier, Sandra
collection PubMed
description Selenoprotein P (SELENOP) is a liver-derived transporter of selenium (Se) in blood, and a meaningful biomarker of Se status. Se is an essential trace element for the biosynthesis of enzymatically-active selenoproteins, protecting the organism from oxidative damage. The usage of uncalibrated assays hinders the comparability of SELENOP concentrations and their pathophysiological interpretation across different clinical studies. On this account, we established a new sandwich SELENOP-ELISA and calibrated against a standard reference material (SRM1950). The ELISA displays a wide working range (11.6–538.4 µg/L), high accuracy (2.9%) and good precision (9.3%). To verify whether SELENOP correlates to total Se and to SELENOP-bound Se, serum samples from healthy subjects and age-selected participants from the Berlin Aging Study II were analyzed by SELENOP-ELISA and Se quantification. SELENOP was affinity-purified and its Se content was determined from a subset of samples. There was a high correlation of total Se and SELENOP concentrations in young and elderly men, and in elderly women, but not in young women, indicating a specific sexual dimorphism in these biomarkers of Se status in young subjects. The Se content of isolated SELENOP was independent of sex and age (mean±SD: 5.4±0.5). By using this calibrated SELENOP-ELISA, prior reports on pathological SELENOP concentrations in diabetes and obesity are challenged as the reported values are outside reasonable limits. Biomarkers of Se status in clinical research need to be measured by validated assays in order to avoid erroneous data and incorrect interpretations, especially when analyzing young women. The Se content of circulating SELENOP differs between individuals and may provide some important diagnostic information on Se metabolism and status.
format Online
Article
Text
id pubmed-5220167
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-52201672017-01-25 Sex-specific and inter-individual differences in biomarkers of selenium status identified by a calibrated ELISA for selenoprotein P Hybsier, Sandra Schulz, Torsten Wu, Zida Demuth, Ilja Minich, Waldemar B. Renko, Kostja Rijntjes, Eddy Köhrle, Josef Strasburger, Christian J. Steinhagen-Thiessen, Elisabeth Schomburg, Lutz Redox Biol Research Paper Selenoprotein P (SELENOP) is a liver-derived transporter of selenium (Se) in blood, and a meaningful biomarker of Se status. Se is an essential trace element for the biosynthesis of enzymatically-active selenoproteins, protecting the organism from oxidative damage. The usage of uncalibrated assays hinders the comparability of SELENOP concentrations and their pathophysiological interpretation across different clinical studies. On this account, we established a new sandwich SELENOP-ELISA and calibrated against a standard reference material (SRM1950). The ELISA displays a wide working range (11.6–538.4 µg/L), high accuracy (2.9%) and good precision (9.3%). To verify whether SELENOP correlates to total Se and to SELENOP-bound Se, serum samples from healthy subjects and age-selected participants from the Berlin Aging Study II were analyzed by SELENOP-ELISA and Se quantification. SELENOP was affinity-purified and its Se content was determined from a subset of samples. There was a high correlation of total Se and SELENOP concentrations in young and elderly men, and in elderly women, but not in young women, indicating a specific sexual dimorphism in these biomarkers of Se status in young subjects. The Se content of isolated SELENOP was independent of sex and age (mean±SD: 5.4±0.5). By using this calibrated SELENOP-ELISA, prior reports on pathological SELENOP concentrations in diabetes and obesity are challenged as the reported values are outside reasonable limits. Biomarkers of Se status in clinical research need to be measured by validated assays in order to avoid erroneous data and incorrect interpretations, especially when analyzing young women. The Se content of circulating SELENOP differs between individuals and may provide some important diagnostic information on Se metabolism and status. Elsevier 2016-12-29 /pmc/articles/PMC5220167/ /pubmed/28064116 http://dx.doi.org/10.1016/j.redox.2016.12.025 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Hybsier, Sandra
Schulz, Torsten
Wu, Zida
Demuth, Ilja
Minich, Waldemar B.
Renko, Kostja
Rijntjes, Eddy
Köhrle, Josef
Strasburger, Christian J.
Steinhagen-Thiessen, Elisabeth
Schomburg, Lutz
Sex-specific and inter-individual differences in biomarkers of selenium status identified by a calibrated ELISA for selenoprotein P
title Sex-specific and inter-individual differences in biomarkers of selenium status identified by a calibrated ELISA for selenoprotein P
title_full Sex-specific and inter-individual differences in biomarkers of selenium status identified by a calibrated ELISA for selenoprotein P
title_fullStr Sex-specific and inter-individual differences in biomarkers of selenium status identified by a calibrated ELISA for selenoprotein P
title_full_unstemmed Sex-specific and inter-individual differences in biomarkers of selenium status identified by a calibrated ELISA for selenoprotein P
title_short Sex-specific and inter-individual differences in biomarkers of selenium status identified by a calibrated ELISA for selenoprotein P
title_sort sex-specific and inter-individual differences in biomarkers of selenium status identified by a calibrated elisa for selenoprotein p
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5220167/
https://www.ncbi.nlm.nih.gov/pubmed/28064116
http://dx.doi.org/10.1016/j.redox.2016.12.025
work_keys_str_mv AT hybsiersandra sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp
AT schulztorsten sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp
AT wuzida sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp
AT demuthilja sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp
AT minichwaldemarb sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp
AT renkokostja sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp
AT rijntjeseddy sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp
AT kohrlejosef sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp
AT strasburgerchristianj sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp
AT steinhagenthiessenelisabeth sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp
AT schomburglutz sexspecificandinterindividualdifferencesinbiomarkersofseleniumstatusidentifiedbyacalibratedelisaforselenoproteinp