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Validation of reference genes for cryopreservation studies with the gorgonian coral endosymbiont Symbiodinium

Quantification by real-time RT-PCR requires a stable internal reference known as a housekeeping gene (HKG) for normalising the mRNA levels of target genes. The present study identified and validated stably expressed HKGs in post-thaw Symbiodinium clade G. Six potential HKGs, namely, pcna, gapdh, 18S...

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Detalles Bibliográficos
Autores principales: Chong, Gabriella, Kuo, Fu-Wen, Tsai, Sujune, Lin, Chiahsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5220285/
https://www.ncbi.nlm.nih.gov/pubmed/28067273
http://dx.doi.org/10.1038/srep39396