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Validation of reference genes for cryopreservation studies with the gorgonian coral endosymbiont Symbiodinium
Quantification by real-time RT-PCR requires a stable internal reference known as a housekeeping gene (HKG) for normalising the mRNA levels of target genes. The present study identified and validated stably expressed HKGs in post-thaw Symbiodinium clade G. Six potential HKGs, namely, pcna, gapdh, 18S...
Autores principales: | Chong, Gabriella, Kuo, Fu-Wen, Tsai, Sujune, Lin, Chiahsin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5220285/ https://www.ncbi.nlm.nih.gov/pubmed/28067273 http://dx.doi.org/10.1038/srep39396 |
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