Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125

Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had...

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Autores principales: Gao, Junkai, Jiang, Yanjun, Lu, Jinshu, Han, Zhi, Deng, Jiajia, Chen, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5220347/
https://www.ncbi.nlm.nih.gov/pubmed/28067335
http://dx.doi.org/10.1038/srep40395
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author Gao, Junkai
Jiang, Yanjun
Lu, Jinshu
Han, Zhi
Deng, Jiajia
Chen, Yan
author_facet Gao, Junkai
Jiang, Yanjun
Lu, Jinshu
Han, Zhi
Deng, Jiajia
Chen, Yan
author_sort Gao, Junkai
collection PubMed
description Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had clear multishell structures with large surface area of 419 m(2)/g. The activity, pH stability, thermal stability, storage stability, and reusability of the LCS@DPMS were investigated in detail. The stabilities of LCS@DPMS were improved significantly compared to the free lipase and LCS@MS (LCS immobilized in unfunctionalized mesoporous onion-like silica by physical adsorption). All the results indicated that the DPMS had high efficiency and improved stability for lipase immobilization.
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spelling pubmed-52203472017-01-11 Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125 Gao, Junkai Jiang, Yanjun Lu, Jinshu Han, Zhi Deng, Jiajia Chen, Yan Sci Rep Article Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had clear multishell structures with large surface area of 419 m(2)/g. The activity, pH stability, thermal stability, storage stability, and reusability of the LCS@DPMS were investigated in detail. The stabilities of LCS@DPMS were improved significantly compared to the free lipase and LCS@MS (LCS immobilized in unfunctionalized mesoporous onion-like silica by physical adsorption). All the results indicated that the DPMS had high efficiency and improved stability for lipase immobilization. Nature Publishing Group 2017-01-09 /pmc/articles/PMC5220347/ /pubmed/28067335 http://dx.doi.org/10.1038/srep40395 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Gao, Junkai
Jiang, Yanjun
Lu, Jinshu
Han, Zhi
Deng, Jiajia
Chen, Yan
Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125
title Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125
title_full Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125
title_fullStr Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125
title_full_unstemmed Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125
title_short Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125
title_sort dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase candida sp. 99-125
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5220347/
https://www.ncbi.nlm.nih.gov/pubmed/28067335
http://dx.doi.org/10.1038/srep40395
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