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Deregulated Cardiac Specific MicroRNAs in Postnatal Heart Growth
The heart is recognized as an organ that is terminally differentiated by adulthood. However, during the process of human development, the heart is the first organ with function in the embryo and grows rapidly during the postnatal period. MicroRNAs (miRNAs, miRs), as regulators of gene expression, pl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5220435/ https://www.ncbi.nlm.nih.gov/pubmed/28105427 http://dx.doi.org/10.1155/2016/6241763 |
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author | Yu, Pujiao Wang, Hongbao Xie, Yuan Zhou, Jinzhe Yao, Jianhua Che, Lin |
author_facet | Yu, Pujiao Wang, Hongbao Xie, Yuan Zhou, Jinzhe Yao, Jianhua Che, Lin |
author_sort | Yu, Pujiao |
collection | PubMed |
description | The heart is recognized as an organ that is terminally differentiated by adulthood. However, during the process of human development, the heart is the first organ with function in the embryo and grows rapidly during the postnatal period. MicroRNAs (miRNAs, miRs), as regulators of gene expression, play important roles during the development of multiple systems. However, the role of miRNAs in postnatal heart growth is still unclear. In this study, by using qRT-PCR, we compared the expression of seven cardiac- or muscle-specific miRNAs that may be related to heart development in heart tissue from mice at postnatal days 0, 3, 8, and 14. Four miRNAs—miR-1a-3p, miR-133b-3p, miR-208b-3p, and miR-206-3p—were significantly decreased while miR-208a-3p was upregulated during the postnatal heart growth period. Based on these results, GeneSpring GX was used to predict potential downstream targets by performing a 3-way comparison of predictions from the miRWalk, PITA, and microRNAorg databases. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used to identify potential functional annotations and signaling pathways related to postnatal heart growth. This study describes expression changes of cardiac- and muscle-specific miRNAs during postnatal heart growth and may provide new therapeutic targets for cardiovascular diseases. |
format | Online Article Text |
id | pubmed-5220435 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-52204352017-01-19 Deregulated Cardiac Specific MicroRNAs in Postnatal Heart Growth Yu, Pujiao Wang, Hongbao Xie, Yuan Zhou, Jinzhe Yao, Jianhua Che, Lin Biomed Res Int Research Article The heart is recognized as an organ that is terminally differentiated by adulthood. However, during the process of human development, the heart is the first organ with function in the embryo and grows rapidly during the postnatal period. MicroRNAs (miRNAs, miRs), as regulators of gene expression, play important roles during the development of multiple systems. However, the role of miRNAs in postnatal heart growth is still unclear. In this study, by using qRT-PCR, we compared the expression of seven cardiac- or muscle-specific miRNAs that may be related to heart development in heart tissue from mice at postnatal days 0, 3, 8, and 14. Four miRNAs—miR-1a-3p, miR-133b-3p, miR-208b-3p, and miR-206-3p—were significantly decreased while miR-208a-3p was upregulated during the postnatal heart growth period. Based on these results, GeneSpring GX was used to predict potential downstream targets by performing a 3-way comparison of predictions from the miRWalk, PITA, and microRNAorg databases. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used to identify potential functional annotations and signaling pathways related to postnatal heart growth. This study describes expression changes of cardiac- and muscle-specific miRNAs during postnatal heart growth and may provide new therapeutic targets for cardiovascular diseases. Hindawi Publishing Corporation 2016 2016-12-25 /pmc/articles/PMC5220435/ /pubmed/28105427 http://dx.doi.org/10.1155/2016/6241763 Text en Copyright © 2016 Pujiao Yu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yu, Pujiao Wang, Hongbao Xie, Yuan Zhou, Jinzhe Yao, Jianhua Che, Lin Deregulated Cardiac Specific MicroRNAs in Postnatal Heart Growth |
title | Deregulated Cardiac Specific MicroRNAs in Postnatal Heart Growth |
title_full | Deregulated Cardiac Specific MicroRNAs in Postnatal Heart Growth |
title_fullStr | Deregulated Cardiac Specific MicroRNAs in Postnatal Heart Growth |
title_full_unstemmed | Deregulated Cardiac Specific MicroRNAs in Postnatal Heart Growth |
title_short | Deregulated Cardiac Specific MicroRNAs in Postnatal Heart Growth |
title_sort | deregulated cardiac specific micrornas in postnatal heart growth |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5220435/ https://www.ncbi.nlm.nih.gov/pubmed/28105427 http://dx.doi.org/10.1155/2016/6241763 |
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