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Multi-casting approach for vascular networks in cellularized hydrogels

Vascularization is essential for living tissue and remains a major challenge in the field of tissue engineering. A lack of a perfusable channel network within a large and densely populated tissue engineered construct leads to necrotic core formation, preventing fabrication of functional tissues and...

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Detalles Bibliográficos
Autores principales: Justin, Alexander W., Brooks, Roger A., Markaki, Athina E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221527/
https://www.ncbi.nlm.nih.gov/pubmed/27928031
http://dx.doi.org/10.1098/rsif.2016.0768
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author Justin, Alexander W.
Brooks, Roger A.
Markaki, Athina E.
author_facet Justin, Alexander W.
Brooks, Roger A.
Markaki, Athina E.
author_sort Justin, Alexander W.
collection PubMed
description Vascularization is essential for living tissue and remains a major challenge in the field of tissue engineering. A lack of a perfusable channel network within a large and densely populated tissue engineered construct leads to necrotic core formation, preventing fabrication of functional tissues and organs. We report a new method for producing a hierarchical, three-dimensional (3D) and perfusable vasculature in a large, cellularized fibrin hydrogel. Bifurcating channels, varying in size from 1 mm to 200–250 µm, are formed using a novel process in which we convert a 3D printed thermoplastic material into a gelatin network template, by way of an intermediate alginate hydrogel. This enables a CAD-based model design, which is highly customizable, reproducible, and which can yield highly complex architectures, to be made into a removable material, which can be used in cellular environments. Our approach yields constructs with a uniform and high density of cells in the bulk, made from bioactive collagen and fibrin hydrogels. Using standard cell staining and immuno-histochemistry techniques, we showed good cell seeding and the presence of tight junctions between channel endothelial cells, and high cell viability and cell spreading in the bulk hydrogel.
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spelling pubmed-52215272017-01-10 Multi-casting approach for vascular networks in cellularized hydrogels Justin, Alexander W. Brooks, Roger A. Markaki, Athina E. J R Soc Interface Life Sciences–Engineering interface Vascularization is essential for living tissue and remains a major challenge in the field of tissue engineering. A lack of a perfusable channel network within a large and densely populated tissue engineered construct leads to necrotic core formation, preventing fabrication of functional tissues and organs. We report a new method for producing a hierarchical, three-dimensional (3D) and perfusable vasculature in a large, cellularized fibrin hydrogel. Bifurcating channels, varying in size from 1 mm to 200–250 µm, are formed using a novel process in which we convert a 3D printed thermoplastic material into a gelatin network template, by way of an intermediate alginate hydrogel. This enables a CAD-based model design, which is highly customizable, reproducible, and which can yield highly complex architectures, to be made into a removable material, which can be used in cellular environments. Our approach yields constructs with a uniform and high density of cells in the bulk, made from bioactive collagen and fibrin hydrogels. Using standard cell staining and immuno-histochemistry techniques, we showed good cell seeding and the presence of tight junctions between channel endothelial cells, and high cell viability and cell spreading in the bulk hydrogel. The Royal Society 2016-12 /pmc/articles/PMC5221527/ /pubmed/27928031 http://dx.doi.org/10.1098/rsif.2016.0768 Text en © 2016 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Life Sciences–Engineering interface
Justin, Alexander W.
Brooks, Roger A.
Markaki, Athina E.
Multi-casting approach for vascular networks in cellularized hydrogels
title Multi-casting approach for vascular networks in cellularized hydrogels
title_full Multi-casting approach for vascular networks in cellularized hydrogels
title_fullStr Multi-casting approach for vascular networks in cellularized hydrogels
title_full_unstemmed Multi-casting approach for vascular networks in cellularized hydrogels
title_short Multi-casting approach for vascular networks in cellularized hydrogels
title_sort multi-casting approach for vascular networks in cellularized hydrogels
topic Life Sciences–Engineering interface
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221527/
https://www.ncbi.nlm.nih.gov/pubmed/27928031
http://dx.doi.org/10.1098/rsif.2016.0768
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