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Cell density and actomyosin contractility control the organization of migrating collectives within an epithelium

The mechanisms underlying collective migration are important for understanding development, wound healing, and tumor invasion. Here we focus on cell density to determine its role in collective migration. Our findings show that increasing cell density, as might be seen in cancer, transforms groups fr...

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Detalles Bibliográficos
Autores principales: Loza, Andrew J., Koride, Sarita, Schimizzi, Gregory V., Li, Bo, Sun, Sean X., Longmore, Gregory D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221580/
https://www.ncbi.nlm.nih.gov/pubmed/27605707
http://dx.doi.org/10.1091/mbc.E16-05-0329
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author Loza, Andrew J.
Koride, Sarita
Schimizzi, Gregory V.
Li, Bo
Sun, Sean X.
Longmore, Gregory D.
author_facet Loza, Andrew J.
Koride, Sarita
Schimizzi, Gregory V.
Li, Bo
Sun, Sean X.
Longmore, Gregory D.
author_sort Loza, Andrew J.
collection PubMed
description The mechanisms underlying collective migration are important for understanding development, wound healing, and tumor invasion. Here we focus on cell density to determine its role in collective migration. Our findings show that increasing cell density, as might be seen in cancer, transforms groups from broad collectives to small, narrow streams. Conversely, diminishing cell density, as might occur at a wound front, leads to large, broad collectives with a distinct leader–follower structure. Simulations identify force-sensitive contractility as a mediator of how density affects collectives, and guided by this prediction, we find that the baseline state of contractility can enhance or reduce organization. Finally, we test predictions from these data in an in vivo epithelium by using genetic manipulations to drive collective motion between predicted migratory phases. This work demonstrates how commonly altered cellular properties can prime groups of cells to adopt migration patterns that may be harnessed in health or exploited in disease.
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spelling pubmed-52215802017-01-22 Cell density and actomyosin contractility control the organization of migrating collectives within an epithelium Loza, Andrew J. Koride, Sarita Schimizzi, Gregory V. Li, Bo Sun, Sean X. Longmore, Gregory D. Mol Biol Cell Articles The mechanisms underlying collective migration are important for understanding development, wound healing, and tumor invasion. Here we focus on cell density to determine its role in collective migration. Our findings show that increasing cell density, as might be seen in cancer, transforms groups from broad collectives to small, narrow streams. Conversely, diminishing cell density, as might occur at a wound front, leads to large, broad collectives with a distinct leader–follower structure. Simulations identify force-sensitive contractility as a mediator of how density affects collectives, and guided by this prediction, we find that the baseline state of contractility can enhance or reduce organization. Finally, we test predictions from these data in an in vivo epithelium by using genetic manipulations to drive collective motion between predicted migratory phases. This work demonstrates how commonly altered cellular properties can prime groups of cells to adopt migration patterns that may be harnessed in health or exploited in disease. The American Society for Cell Biology 2016-11-07 /pmc/articles/PMC5221580/ /pubmed/27605707 http://dx.doi.org/10.1091/mbc.E16-05-0329 Text en © 2016 Loza et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Loza, Andrew J.
Koride, Sarita
Schimizzi, Gregory V.
Li, Bo
Sun, Sean X.
Longmore, Gregory D.
Cell density and actomyosin contractility control the organization of migrating collectives within an epithelium
title Cell density and actomyosin contractility control the organization of migrating collectives within an epithelium
title_full Cell density and actomyosin contractility control the organization of migrating collectives within an epithelium
title_fullStr Cell density and actomyosin contractility control the organization of migrating collectives within an epithelium
title_full_unstemmed Cell density and actomyosin contractility control the organization of migrating collectives within an epithelium
title_short Cell density and actomyosin contractility control the organization of migrating collectives within an epithelium
title_sort cell density and actomyosin contractility control the organization of migrating collectives within an epithelium
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221580/
https://www.ncbi.nlm.nih.gov/pubmed/27605707
http://dx.doi.org/10.1091/mbc.E16-05-0329
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