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Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast

Kinesin-8 motor proteins destabilize microtubules. Their absence during cell division is associated with disorganized mitotic chromosome movements and chromosome loss. Despite recent work studying effects of kinesin-8s on microtubule dynamics, it remains unclear whether the kinesin-8 mitotic phenoty...

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Autores principales: Gergely, Zachary R., Crapo, Ammon, Hough, Loren E., McIntosh, J. Richard, Betterton, Meredith D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221583/
https://www.ncbi.nlm.nih.gov/pubmed/27146110
http://dx.doi.org/10.1091/mbc.E15-07-0505
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author Gergely, Zachary R.
Crapo, Ammon
Hough, Loren E.
McIntosh, J. Richard
Betterton, Meredith D.
author_facet Gergely, Zachary R.
Crapo, Ammon
Hough, Loren E.
McIntosh, J. Richard
Betterton, Meredith D.
author_sort Gergely, Zachary R.
collection PubMed
description Kinesin-8 motor proteins destabilize microtubules. Their absence during cell division is associated with disorganized mitotic chromosome movements and chromosome loss. Despite recent work studying effects of kinesin-8s on microtubule dynamics, it remains unclear whether the kinesin-8 mitotic phenotypes are consequences of their effect on microtubule dynamics, their well-established motor activity, or additional, unknown functions. To better understand the role of kinesin-8 proteins in mitosis, we studied the effects of deletion of the fission yeast kinesin-8 proteins Klp5 and Klp6 on chromosome movements and spindle length dynamics. Aberrant microtubule-driven kinetochore pushing movements and tripolar mitotic spindles occurred in cells lacking Klp5 but not Klp6. Kinesin-8–deletion strains showed large fluctuations in metaphase spindle length, suggesting a disruption of spindle length stabilization. Comparison of our results from light microscopy with a mathematical model suggests that kinesin-8–induced effects on microtubule dynamics, kinetochore attachment stability, and sliding force in the spindle can explain the aberrant chromosome movements and spindle length fluctuations seen.
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spelling pubmed-52215832017-01-22 Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast Gergely, Zachary R. Crapo, Ammon Hough, Loren E. McIntosh, J. Richard Betterton, Meredith D. Mol Biol Cell Articles Kinesin-8 motor proteins destabilize microtubules. Their absence during cell division is associated with disorganized mitotic chromosome movements and chromosome loss. Despite recent work studying effects of kinesin-8s on microtubule dynamics, it remains unclear whether the kinesin-8 mitotic phenotypes are consequences of their effect on microtubule dynamics, their well-established motor activity, or additional, unknown functions. To better understand the role of kinesin-8 proteins in mitosis, we studied the effects of deletion of the fission yeast kinesin-8 proteins Klp5 and Klp6 on chromosome movements and spindle length dynamics. Aberrant microtubule-driven kinetochore pushing movements and tripolar mitotic spindles occurred in cells lacking Klp5 but not Klp6. Kinesin-8–deletion strains showed large fluctuations in metaphase spindle length, suggesting a disruption of spindle length stabilization. Comparison of our results from light microscopy with a mathematical model suggests that kinesin-8–induced effects on microtubule dynamics, kinetochore attachment stability, and sliding force in the spindle can explain the aberrant chromosome movements and spindle length fluctuations seen. The American Society for Cell Biology 2016-11-07 /pmc/articles/PMC5221583/ /pubmed/27146110 http://dx.doi.org/10.1091/mbc.E15-07-0505 Text en © 2016 Gergely et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Gergely, Zachary R.
Crapo, Ammon
Hough, Loren E.
McIntosh, J. Richard
Betterton, Meredith D.
Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast
title Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast
title_full Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast
title_fullStr Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast
title_full_unstemmed Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast
title_short Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast
title_sort kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221583/
https://www.ncbi.nlm.nih.gov/pubmed/27146110
http://dx.doi.org/10.1091/mbc.E15-07-0505
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