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A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies

Control of organellar assembly and function is critical to eukaryotic homeostasis and survival. Gle1 is a highly conserved regulator of RNA-dependent DEAD-box ATPase proteins, with critical roles in both mRNA export and translation. In addition to its well-defined interaction with nuclear pore compl...

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Autores principales: Jao, Li-En, Akef, Abdalla, Wente, Susan R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221616/
https://www.ncbi.nlm.nih.gov/pubmed/28035044
http://dx.doi.org/10.1091/mbc.E16-09-0675
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author Jao, Li-En
Akef, Abdalla
Wente, Susan R.
author_facet Jao, Li-En
Akef, Abdalla
Wente, Susan R.
author_sort Jao, Li-En
collection PubMed
description Control of organellar assembly and function is critical to eukaryotic homeostasis and survival. Gle1 is a highly conserved regulator of RNA-dependent DEAD-box ATPase proteins, with critical roles in both mRNA export and translation. In addition to its well-defined interaction with nuclear pore complexes, here we find that Gle1 is enriched at the centrosome and basal body. Gle1 assembles into the toroid-shaped pericentriolar material around the mother centriole. Reduced Gle1 levels are correlated with decreased pericentrin localization at the centrosome and microtubule organization defects. Of importance, these alterations in centrosome integrity do not result from loss of mRNA export. Examination of the Kupffer’s vesicle in Gle1-depleted zebrafish revealed compromised ciliary beating and developmental defects. We propose that Gle1 assembly into the pericentriolar material positions the DEAD-box protein regulator to function in localized mRNA metabolism required for proper centrosome function.
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spelling pubmed-52216162017-03-16 A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies Jao, Li-En Akef, Abdalla Wente, Susan R. Mol Biol Cell Articles Control of organellar assembly and function is critical to eukaryotic homeostasis and survival. Gle1 is a highly conserved regulator of RNA-dependent DEAD-box ATPase proteins, with critical roles in both mRNA export and translation. In addition to its well-defined interaction with nuclear pore complexes, here we find that Gle1 is enriched at the centrosome and basal body. Gle1 assembles into the toroid-shaped pericentriolar material around the mother centriole. Reduced Gle1 levels are correlated with decreased pericentrin localization at the centrosome and microtubule organization defects. Of importance, these alterations in centrosome integrity do not result from loss of mRNA export. Examination of the Kupffer’s vesicle in Gle1-depleted zebrafish revealed compromised ciliary beating and developmental defects. We propose that Gle1 assembly into the pericentriolar material positions the DEAD-box protein regulator to function in localized mRNA metabolism required for proper centrosome function. The American Society for Cell Biology 2017-01-01 /pmc/articles/PMC5221616/ /pubmed/28035044 http://dx.doi.org/10.1091/mbc.E16-09-0675 Text en © 2017 Jao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Jao, Li-En
Akef, Abdalla
Wente, Susan R.
A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies
title A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies
title_full A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies
title_fullStr A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies
title_full_unstemmed A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies
title_short A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies
title_sort role for gle1, a regulator of dead-box rna helicases, at centrosomes and basal bodies
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221616/
https://www.ncbi.nlm.nih.gov/pubmed/28035044
http://dx.doi.org/10.1091/mbc.E16-09-0675
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