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Molecular and Functional Characterization of GR2-R1 Event Based Backcross Derived Lines of Golden Rice in the Genetic Background of a Mega Rice Variety Swarna

Homozygous Golden Rice lines developed in the background of Swarna through marker assisted backcross breeding (MABB) using transgenic GR2-R1 event as a donor for the provitamin A trait have high levels of provitamin A (up to 20 ppm) but are dwarf with pale green leaves and drastically reduced panicl...

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Autores principales: Bollinedi, Haritha, S., Gopala Krishnan, Prabhu, Kumble Vinod, Singh, Nagendra Kumar, Mishra, Sushma, Khurana, Jitendra P., Singh, Ashok Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221763/
https://www.ncbi.nlm.nih.gov/pubmed/28068433
http://dx.doi.org/10.1371/journal.pone.0169600
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author Bollinedi, Haritha
S., Gopala Krishnan
Prabhu, Kumble Vinod
Singh, Nagendra Kumar
Mishra, Sushma
Khurana, Jitendra P.
Singh, Ashok Kumar
author_facet Bollinedi, Haritha
S., Gopala Krishnan
Prabhu, Kumble Vinod
Singh, Nagendra Kumar
Mishra, Sushma
Khurana, Jitendra P.
Singh, Ashok Kumar
author_sort Bollinedi, Haritha
collection PubMed
description Homozygous Golden Rice lines developed in the background of Swarna through marker assisted backcross breeding (MABB) using transgenic GR2-R1 event as a donor for the provitamin A trait have high levels of provitamin A (up to 20 ppm) but are dwarf with pale green leaves and drastically reduced panicle size, grain number and yield as compared to the recurrent parent, Swarna. In this study, we carried out detailed morphological, biochemical and molecular characterization of these lines in a quest to identify the probable reasons for their abnormal phenotype. Nucleotide blast analysis with the primer sequences used to amplify the transgene revealed that the integration of transgene disrupted the native OsAux1 gene, which codes for an auxin transmembrane transporter protein. Real time expression analysis of the transgenes (ZmPsy and CrtI) driven by endosperm-specific promoter revealed the leaky expression of the transgene in the vegetative tissues. We propose that the disruption of OsAux1 disturbed the fine balance of plant growth regulators viz., auxins, gibberellic acid and abscisic acid, leading to the abnormalities in the growth and development of the lines homozygous for the transgene. The study demonstrates the conserved roles of OsAux1 gene in rice and Arabidopsis.
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spelling pubmed-52217632017-01-19 Molecular and Functional Characterization of GR2-R1 Event Based Backcross Derived Lines of Golden Rice in the Genetic Background of a Mega Rice Variety Swarna Bollinedi, Haritha S., Gopala Krishnan Prabhu, Kumble Vinod Singh, Nagendra Kumar Mishra, Sushma Khurana, Jitendra P. Singh, Ashok Kumar PLoS One Research Article Homozygous Golden Rice lines developed in the background of Swarna through marker assisted backcross breeding (MABB) using transgenic GR2-R1 event as a donor for the provitamin A trait have high levels of provitamin A (up to 20 ppm) but are dwarf with pale green leaves and drastically reduced panicle size, grain number and yield as compared to the recurrent parent, Swarna. In this study, we carried out detailed morphological, biochemical and molecular characterization of these lines in a quest to identify the probable reasons for their abnormal phenotype. Nucleotide blast analysis with the primer sequences used to amplify the transgene revealed that the integration of transgene disrupted the native OsAux1 gene, which codes for an auxin transmembrane transporter protein. Real time expression analysis of the transgenes (ZmPsy and CrtI) driven by endosperm-specific promoter revealed the leaky expression of the transgene in the vegetative tissues. We propose that the disruption of OsAux1 disturbed the fine balance of plant growth regulators viz., auxins, gibberellic acid and abscisic acid, leading to the abnormalities in the growth and development of the lines homozygous for the transgene. The study demonstrates the conserved roles of OsAux1 gene in rice and Arabidopsis. Public Library of Science 2017-01-09 /pmc/articles/PMC5221763/ /pubmed/28068433 http://dx.doi.org/10.1371/journal.pone.0169600 Text en © 2017 Bollinedi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bollinedi, Haritha
S., Gopala Krishnan
Prabhu, Kumble Vinod
Singh, Nagendra Kumar
Mishra, Sushma
Khurana, Jitendra P.
Singh, Ashok Kumar
Molecular and Functional Characterization of GR2-R1 Event Based Backcross Derived Lines of Golden Rice in the Genetic Background of a Mega Rice Variety Swarna
title Molecular and Functional Characterization of GR2-R1 Event Based Backcross Derived Lines of Golden Rice in the Genetic Background of a Mega Rice Variety Swarna
title_full Molecular and Functional Characterization of GR2-R1 Event Based Backcross Derived Lines of Golden Rice in the Genetic Background of a Mega Rice Variety Swarna
title_fullStr Molecular and Functional Characterization of GR2-R1 Event Based Backcross Derived Lines of Golden Rice in the Genetic Background of a Mega Rice Variety Swarna
title_full_unstemmed Molecular and Functional Characterization of GR2-R1 Event Based Backcross Derived Lines of Golden Rice in the Genetic Background of a Mega Rice Variety Swarna
title_short Molecular and Functional Characterization of GR2-R1 Event Based Backcross Derived Lines of Golden Rice in the Genetic Background of a Mega Rice Variety Swarna
title_sort molecular and functional characterization of gr2-r1 event based backcross derived lines of golden rice in the genetic background of a mega rice variety swarna
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221763/
https://www.ncbi.nlm.nih.gov/pubmed/28068433
http://dx.doi.org/10.1371/journal.pone.0169600
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