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Sequential formation of two branched intermediates during protein splicing of class three inteins
Inteins are the protein equivalent of introns. They are seamlessly removed during post-translational maturation of their host protein (extein). Inteins from extremophiles played a key role in understanding intein-mediated protein splicing. There are currently three classes of inteins defined by cata...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Japan
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5222942/ https://www.ncbi.nlm.nih.gov/pubmed/27704298 http://dx.doi.org/10.1007/s00792-016-0876-0 |
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author | Tori, Kazuo Perler, Francine |
author_facet | Tori, Kazuo Perler, Francine |
author_sort | Tori, Kazuo |
collection | PubMed |
description | Inteins are the protein equivalent of introns. They are seamlessly removed during post-translational maturation of their host protein (extein). Inteins from extremophiles played a key role in understanding intein-mediated protein splicing. There are currently three classes of inteins defined by catalytic mechanism and sequence signatures. This study demonstrates splicing of three class 3 mini-inteins: Burkholderia vietnamiensis G4 Bvi IcmO intein, Mycobacterium smegmatis MC2 155 Msm DnaB-1 intein and Mycobacterium leprae strain TN Mle DnaB intein. B. vietnamiensis has a broad ecological range and remediates trichloroethene. M. smegmatis is a biofilm forming soil bacteria. Although other intein classes have only a single branched intermediate at the C-terminal splice junction, the class 3 intein reaction pathway includes two branched intermediates. The class 3 specific branched intermediate is formed by an internal cysteine, while the C-terminal branch intermediate is at a serine or threonine in all class 3 inteins except the Bvi IcmO intein, where it is a cysteine. This latter cysteine was unable to compensate for mutation of the class 3-specific internal catalytic cysteine despite the Bvi IcmO intein having an N-terminal splice junction naturally tuned for a cysteine nucleophile, demonstrating the mandatory order of branch intermediates in class 3 inteins. |
format | Online Article Text |
id | pubmed-5222942 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-52229422017-01-19 Sequential formation of two branched intermediates during protein splicing of class three inteins Tori, Kazuo Perler, Francine Extremophiles Special Feature: Original Paper Inteins are the protein equivalent of introns. They are seamlessly removed during post-translational maturation of their host protein (extein). Inteins from extremophiles played a key role in understanding intein-mediated protein splicing. There are currently three classes of inteins defined by catalytic mechanism and sequence signatures. This study demonstrates splicing of three class 3 mini-inteins: Burkholderia vietnamiensis G4 Bvi IcmO intein, Mycobacterium smegmatis MC2 155 Msm DnaB-1 intein and Mycobacterium leprae strain TN Mle DnaB intein. B. vietnamiensis has a broad ecological range and remediates trichloroethene. M. smegmatis is a biofilm forming soil bacteria. Although other intein classes have only a single branched intermediate at the C-terminal splice junction, the class 3 intein reaction pathway includes two branched intermediates. The class 3 specific branched intermediate is formed by an internal cysteine, while the C-terminal branch intermediate is at a serine or threonine in all class 3 inteins except the Bvi IcmO intein, where it is a cysteine. This latter cysteine was unable to compensate for mutation of the class 3-specific internal catalytic cysteine despite the Bvi IcmO intein having an N-terminal splice junction naturally tuned for a cysteine nucleophile, demonstrating the mandatory order of branch intermediates in class 3 inteins. Springer Japan 2016-10-04 2017 /pmc/articles/PMC5222942/ /pubmed/27704298 http://dx.doi.org/10.1007/s00792-016-0876-0 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Special Feature: Original Paper Tori, Kazuo Perler, Francine Sequential formation of two branched intermediates during protein splicing of class three inteins |
title | Sequential formation of two branched intermediates during protein splicing of class three inteins |
title_full | Sequential formation of two branched intermediates during protein splicing of class three inteins |
title_fullStr | Sequential formation of two branched intermediates during protein splicing of class three inteins |
title_full_unstemmed | Sequential formation of two branched intermediates during protein splicing of class three inteins |
title_short | Sequential formation of two branched intermediates during protein splicing of class three inteins |
title_sort | sequential formation of two branched intermediates during protein splicing of class three inteins |
topic | Special Feature: Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5222942/ https://www.ncbi.nlm.nih.gov/pubmed/27704298 http://dx.doi.org/10.1007/s00792-016-0876-0 |
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