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A next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke Schistosoma japonicum

BACKGROUND: Schistosomiasis is caused by infection with blood flukes of the genus Schistosoma, and ranks, in terms of disability-adjusted life years (DALYs), as the third most important neglected tropical disease. Schistosomes have several discrete life stages involving dramatic morphological change...

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Autores principales: Cai, Pengfei, Liu, Shuai, Piao, Xianyu, Hou, Nan, You, Hong, McManus, Donald P., Chen, Qijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223471/
https://www.ncbi.nlm.nih.gov/pubmed/28069074
http://dx.doi.org/10.1186/s13071-016-1947-x
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author Cai, Pengfei
Liu, Shuai
Piao, Xianyu
Hou, Nan
You, Hong
McManus, Donald P.
Chen, Qijun
author_facet Cai, Pengfei
Liu, Shuai
Piao, Xianyu
Hou, Nan
You, Hong
McManus, Donald P.
Chen, Qijun
author_sort Cai, Pengfei
collection PubMed
description BACKGROUND: Schistosomiasis is caused by infection with blood flukes of the genus Schistosoma, and ranks, in terms of disability-adjusted life years (DALYs), as the third most important neglected tropical disease. Schistosomes have several discrete life stages involving dramatic morphological changes during their development, which require subtle gene expression modulations to complete the complex life-cycle. RESULTS: In the current study, we employed a second generation schistosome DNA chip printed with the most comprehensive probe array for studying the Schistosoma japonicum transcriptome, to explore stage-associated gene expression in different developmental phases of S. japonicum. A total of 328, 95, 268 and 532 mRNA transcripts were enriched in cercariae, hepatic schistosomula, adult worms and eggs, respectively. In general, genes associated with transcriptional regulation, cell signalling and motor activity were readily expressed in cercariae; the expression of genes involved in neuronal activities, apoptosis and renewal was modestly upregulated in hepatic schistosomula; transcripts involved in egg production, nutrition metabolism and glycosylation were enriched in adult worms; while genes involved in cell division, microtubule-associated mobility, and host-parasite interplay were relatively highly expressed in eggs. CONCLUSIONS: The study further highlights the expressional features of stage-associated genes in schistosomes with high accuracy. The results provide a better perspective of the biological characteristics among different developmental stages, which may open new avenues for identification of novel vaccine candidates and the development of novel control interventions against schistosomiasis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1947-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-52234712017-01-11 A next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke Schistosoma japonicum Cai, Pengfei Liu, Shuai Piao, Xianyu Hou, Nan You, Hong McManus, Donald P. Chen, Qijun Parasit Vectors Research BACKGROUND: Schistosomiasis is caused by infection with blood flukes of the genus Schistosoma, and ranks, in terms of disability-adjusted life years (DALYs), as the third most important neglected tropical disease. Schistosomes have several discrete life stages involving dramatic morphological changes during their development, which require subtle gene expression modulations to complete the complex life-cycle. RESULTS: In the current study, we employed a second generation schistosome DNA chip printed with the most comprehensive probe array for studying the Schistosoma japonicum transcriptome, to explore stage-associated gene expression in different developmental phases of S. japonicum. A total of 328, 95, 268 and 532 mRNA transcripts were enriched in cercariae, hepatic schistosomula, adult worms and eggs, respectively. In general, genes associated with transcriptional regulation, cell signalling and motor activity were readily expressed in cercariae; the expression of genes involved in neuronal activities, apoptosis and renewal was modestly upregulated in hepatic schistosomula; transcripts involved in egg production, nutrition metabolism and glycosylation were enriched in adult worms; while genes involved in cell division, microtubule-associated mobility, and host-parasite interplay were relatively highly expressed in eggs. CONCLUSIONS: The study further highlights the expressional features of stage-associated genes in schistosomes with high accuracy. The results provide a better perspective of the biological characteristics among different developmental stages, which may open new avenues for identification of novel vaccine candidates and the development of novel control interventions against schistosomiasis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1947-x) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-10 /pmc/articles/PMC5223471/ /pubmed/28069074 http://dx.doi.org/10.1186/s13071-016-1947-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Cai, Pengfei
Liu, Shuai
Piao, Xianyu
Hou, Nan
You, Hong
McManus, Donald P.
Chen, Qijun
A next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke Schistosoma japonicum
title A next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke Schistosoma japonicum
title_full A next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke Schistosoma japonicum
title_fullStr A next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke Schistosoma japonicum
title_full_unstemmed A next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke Schistosoma japonicum
title_short A next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke Schistosoma japonicum
title_sort next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke schistosoma japonicum
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223471/
https://www.ncbi.nlm.nih.gov/pubmed/28069074
http://dx.doi.org/10.1186/s13071-016-1947-x
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