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Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium

BACKGROUND: Fistular leaves frequently appear in Allium species, and previous developmental studies have proposed that the process of fistular leaf formation involves programmed cell death. However, molecular evidence for the role of programmed cell death in the formation of fistular leaf cavities h...

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Autores principales: Zhu, Siyuan, Tang, Shouwei, Tan, Zhijian, Yu, Yongting, Dai, Qiuzhong, Liu, Touming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223570/
https://www.ncbi.nlm.nih.gov/pubmed/28068920
http://dx.doi.org/10.1186/s12864-016-3474-8
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author Zhu, Siyuan
Tang, Shouwei
Tan, Zhijian
Yu, Yongting
Dai, Qiuzhong
Liu, Touming
author_facet Zhu, Siyuan
Tang, Shouwei
Tan, Zhijian
Yu, Yongting
Dai, Qiuzhong
Liu, Touming
author_sort Zhu, Siyuan
collection PubMed
description BACKGROUND: Fistular leaves frequently appear in Allium species, and previous developmental studies have proposed that the process of fistular leaf formation involves programmed cell death. However, molecular evidence for the role of programmed cell death in the formation of fistular leaf cavities has yet to be reported. RESULTS: In this study, we characterized the leaf transcriptomes of nine Allium species, including six fistular- and three solid-leaved species. In addition, we identified orthologous genes and estimated their Ka and Ks values, in order to ascertain their selective pattern. Phylogenetic analysis based on the transcriptomes revealed that A. tuberosum was the most ancestral among the nine species, and analysis of orthologous genes between A. tuberosum and the other eight species indicated that 149 genes were subject to positive selection; whereas >3000 had undergone purifying selection in each species. CONCLUSIONS: We found that many genes that are potentially related to programmed cell death either exhibited rapid diversification in fistular-leaved species, or were conserved in solid-leaved species in evolutionary history. These genes potentially involved in programmed cell death might play important roles in the formation of fistular leaf cavities in Allium, and the differing selection patterns in fistular- and solid-leaved species may be responsible for the evolution of fistular leaves. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-3474-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-52235702017-01-11 Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium Zhu, Siyuan Tang, Shouwei Tan, Zhijian Yu, Yongting Dai, Qiuzhong Liu, Touming BMC Genomics Research Article BACKGROUND: Fistular leaves frequently appear in Allium species, and previous developmental studies have proposed that the process of fistular leaf formation involves programmed cell death. However, molecular evidence for the role of programmed cell death in the formation of fistular leaf cavities has yet to be reported. RESULTS: In this study, we characterized the leaf transcriptomes of nine Allium species, including six fistular- and three solid-leaved species. In addition, we identified orthologous genes and estimated their Ka and Ks values, in order to ascertain their selective pattern. Phylogenetic analysis based on the transcriptomes revealed that A. tuberosum was the most ancestral among the nine species, and analysis of orthologous genes between A. tuberosum and the other eight species indicated that 149 genes were subject to positive selection; whereas >3000 had undergone purifying selection in each species. CONCLUSIONS: We found that many genes that are potentially related to programmed cell death either exhibited rapid diversification in fistular-leaved species, or were conserved in solid-leaved species in evolutionary history. These genes potentially involved in programmed cell death might play important roles in the formation of fistular leaf cavities in Allium, and the differing selection patterns in fistular- and solid-leaved species may be responsible for the evolution of fistular leaves. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-3474-8) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-10 /pmc/articles/PMC5223570/ /pubmed/28068920 http://dx.doi.org/10.1186/s12864-016-3474-8 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhu, Siyuan
Tang, Shouwei
Tan, Zhijian
Yu, Yongting
Dai, Qiuzhong
Liu, Touming
Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium
title Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium
title_full Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium
title_fullStr Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium
title_full_unstemmed Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium
title_short Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium
title_sort comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in allium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223570/
https://www.ncbi.nlm.nih.gov/pubmed/28068920
http://dx.doi.org/10.1186/s12864-016-3474-8
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