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Constitutive centromere-associated network controls centromere drift in vertebrate cells
Centromeres are specified by sequence-independent epigenetic mechanisms, and the centromere position may drift at each cell cycle, but once this position is specified, it may not be frequently moved. Currently, it is unclear whether the centromere position is stable. To address this question, we sys...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223601/ https://www.ncbi.nlm.nih.gov/pubmed/27940888 http://dx.doi.org/10.1083/jcb.201605001 |
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author | Hori, Tetsuya Kagawa, Naoko Toyoda, Atsushi Fujiyama, Asao Misu, Sadahiko Monma, Norikazu Makino, Fumiaki Ikeo, Kazuho Fukagawa, Tatsuo |
author_facet | Hori, Tetsuya Kagawa, Naoko Toyoda, Atsushi Fujiyama, Asao Misu, Sadahiko Monma, Norikazu Makino, Fumiaki Ikeo, Kazuho Fukagawa, Tatsuo |
author_sort | Hori, Tetsuya |
collection | PubMed |
description | Centromeres are specified by sequence-independent epigenetic mechanisms, and the centromere position may drift at each cell cycle, but once this position is specified, it may not be frequently moved. Currently, it is unclear whether the centromere position is stable. To address this question, we systematically analyzed the position of nonrepetitive centromeres in 21 independent clones isolated from a laboratory stock of chicken DT40 cells using chromatin immunoprecipitation combined with massive parallel sequencing analysis with anti–CENP-A antibody. We demonstrated that the centromere position varies among the clones, suggesting that centromere drift occurs during cell proliferation. However, when we analyzed this position in the subclones obtained from one isolated clone, the position was found to be relatively stable. Interestingly, the centromere drift was shown to occur frequently in CENP-U– and CENP-S–deficient cells. Based on these results, we suggest that the centromere position can change after many cell divisions, but this drift is suppressed in short-term cultures, and the complete centromere structure contributes to the suppression of the centromere drift. |
format | Online Article Text |
id | pubmed-5223601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-52236012017-07-02 Constitutive centromere-associated network controls centromere drift in vertebrate cells Hori, Tetsuya Kagawa, Naoko Toyoda, Atsushi Fujiyama, Asao Misu, Sadahiko Monma, Norikazu Makino, Fumiaki Ikeo, Kazuho Fukagawa, Tatsuo J Cell Biol Research Articles Centromeres are specified by sequence-independent epigenetic mechanisms, and the centromere position may drift at each cell cycle, but once this position is specified, it may not be frequently moved. Currently, it is unclear whether the centromere position is stable. To address this question, we systematically analyzed the position of nonrepetitive centromeres in 21 independent clones isolated from a laboratory stock of chicken DT40 cells using chromatin immunoprecipitation combined with massive parallel sequencing analysis with anti–CENP-A antibody. We demonstrated that the centromere position varies among the clones, suggesting that centromere drift occurs during cell proliferation. However, when we analyzed this position in the subclones obtained from one isolated clone, the position was found to be relatively stable. Interestingly, the centromere drift was shown to occur frequently in CENP-U– and CENP-S–deficient cells. Based on these results, we suggest that the centromere position can change after many cell divisions, but this drift is suppressed in short-term cultures, and the complete centromere structure contributes to the suppression of the centromere drift. The Rockefeller University Press 2017-01-02 /pmc/articles/PMC5223601/ /pubmed/27940888 http://dx.doi.org/10.1083/jcb.201605001 Text en © 2017 Hori et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License(Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Hori, Tetsuya Kagawa, Naoko Toyoda, Atsushi Fujiyama, Asao Misu, Sadahiko Monma, Norikazu Makino, Fumiaki Ikeo, Kazuho Fukagawa, Tatsuo Constitutive centromere-associated network controls centromere drift in vertebrate cells |
title | Constitutive centromere-associated network controls centromere drift in vertebrate cells |
title_full | Constitutive centromere-associated network controls centromere drift in vertebrate cells |
title_fullStr | Constitutive centromere-associated network controls centromere drift in vertebrate cells |
title_full_unstemmed | Constitutive centromere-associated network controls centromere drift in vertebrate cells |
title_short | Constitutive centromere-associated network controls centromere drift in vertebrate cells |
title_sort | constitutive centromere-associated network controls centromere drift in vertebrate cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223601/ https://www.ncbi.nlm.nih.gov/pubmed/27940888 http://dx.doi.org/10.1083/jcb.201605001 |
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