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Constitutive centromere-associated network controls centromere drift in vertebrate cells

Centromeres are specified by sequence-independent epigenetic mechanisms, and the centromere position may drift at each cell cycle, but once this position is specified, it may not be frequently moved. Currently, it is unclear whether the centromere position is stable. To address this question, we sys...

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Autores principales: Hori, Tetsuya, Kagawa, Naoko, Toyoda, Atsushi, Fujiyama, Asao, Misu, Sadahiko, Monma, Norikazu, Makino, Fumiaki, Ikeo, Kazuho, Fukagawa, Tatsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223601/
https://www.ncbi.nlm.nih.gov/pubmed/27940888
http://dx.doi.org/10.1083/jcb.201605001
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author Hori, Tetsuya
Kagawa, Naoko
Toyoda, Atsushi
Fujiyama, Asao
Misu, Sadahiko
Monma, Norikazu
Makino, Fumiaki
Ikeo, Kazuho
Fukagawa, Tatsuo
author_facet Hori, Tetsuya
Kagawa, Naoko
Toyoda, Atsushi
Fujiyama, Asao
Misu, Sadahiko
Monma, Norikazu
Makino, Fumiaki
Ikeo, Kazuho
Fukagawa, Tatsuo
author_sort Hori, Tetsuya
collection PubMed
description Centromeres are specified by sequence-independent epigenetic mechanisms, and the centromere position may drift at each cell cycle, but once this position is specified, it may not be frequently moved. Currently, it is unclear whether the centromere position is stable. To address this question, we systematically analyzed the position of nonrepetitive centromeres in 21 independent clones isolated from a laboratory stock of chicken DT40 cells using chromatin immunoprecipitation combined with massive parallel sequencing analysis with anti–CENP-A antibody. We demonstrated that the centromere position varies among the clones, suggesting that centromere drift occurs during cell proliferation. However, when we analyzed this position in the subclones obtained from one isolated clone, the position was found to be relatively stable. Interestingly, the centromere drift was shown to occur frequently in CENP-U– and CENP-S–deficient cells. Based on these results, we suggest that the centromere position can change after many cell divisions, but this drift is suppressed in short-term cultures, and the complete centromere structure contributes to the suppression of the centromere drift.
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spelling pubmed-52236012017-07-02 Constitutive centromere-associated network controls centromere drift in vertebrate cells Hori, Tetsuya Kagawa, Naoko Toyoda, Atsushi Fujiyama, Asao Misu, Sadahiko Monma, Norikazu Makino, Fumiaki Ikeo, Kazuho Fukagawa, Tatsuo J Cell Biol Research Articles Centromeres are specified by sequence-independent epigenetic mechanisms, and the centromere position may drift at each cell cycle, but once this position is specified, it may not be frequently moved. Currently, it is unclear whether the centromere position is stable. To address this question, we systematically analyzed the position of nonrepetitive centromeres in 21 independent clones isolated from a laboratory stock of chicken DT40 cells using chromatin immunoprecipitation combined with massive parallel sequencing analysis with anti–CENP-A antibody. We demonstrated that the centromere position varies among the clones, suggesting that centromere drift occurs during cell proliferation. However, when we analyzed this position in the subclones obtained from one isolated clone, the position was found to be relatively stable. Interestingly, the centromere drift was shown to occur frequently in CENP-U– and CENP-S–deficient cells. Based on these results, we suggest that the centromere position can change after many cell divisions, but this drift is suppressed in short-term cultures, and the complete centromere structure contributes to the suppression of the centromere drift. The Rockefeller University Press 2017-01-02 /pmc/articles/PMC5223601/ /pubmed/27940888 http://dx.doi.org/10.1083/jcb.201605001 Text en © 2017 Hori et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License(Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Hori, Tetsuya
Kagawa, Naoko
Toyoda, Atsushi
Fujiyama, Asao
Misu, Sadahiko
Monma, Norikazu
Makino, Fumiaki
Ikeo, Kazuho
Fukagawa, Tatsuo
Constitutive centromere-associated network controls centromere drift in vertebrate cells
title Constitutive centromere-associated network controls centromere drift in vertebrate cells
title_full Constitutive centromere-associated network controls centromere drift in vertebrate cells
title_fullStr Constitutive centromere-associated network controls centromere drift in vertebrate cells
title_full_unstemmed Constitutive centromere-associated network controls centromere drift in vertebrate cells
title_short Constitutive centromere-associated network controls centromere drift in vertebrate cells
title_sort constitutive centromere-associated network controls centromere drift in vertebrate cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223601/
https://www.ncbi.nlm.nih.gov/pubmed/27940888
http://dx.doi.org/10.1083/jcb.201605001
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