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Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay

The purpose of this study was to examine whether a simple bioassay used for the detection of dioxins (DXNs) could be applied to detect trace amounts of harmful DXN-like substances in food products. To identify substances with possible DXN-like activity, we assessed the ability of various compounds i...

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Autores principales: Amakura, Yoshiaki, Tsutsumi, Tomoaki, Yoshimura, Morio, Nakamura, Masafumi, Handa, Hiroshi, Matsuda, Rieko, Teshima, Reiko, Watanabe, Takahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224577/
https://www.ncbi.nlm.nih.gov/pubmed/28231110
http://dx.doi.org/10.3390/foods5010015
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author Amakura, Yoshiaki
Tsutsumi, Tomoaki
Yoshimura, Morio
Nakamura, Masafumi
Handa, Hiroshi
Matsuda, Rieko
Teshima, Reiko
Watanabe, Takahiro
author_facet Amakura, Yoshiaki
Tsutsumi, Tomoaki
Yoshimura, Morio
Nakamura, Masafumi
Handa, Hiroshi
Matsuda, Rieko
Teshima, Reiko
Watanabe, Takahiro
author_sort Amakura, Yoshiaki
collection PubMed
description The purpose of this study was to examine whether a simple bioassay used for the detection of dioxins (DXNs) could be applied to detect trace amounts of harmful DXN-like substances in food products. To identify substances with possible DXN-like activity, we assessed the ability of various compounds in the environment to bind the aryl hydrocarbon receptor (AhR) that binds specifically to DXNs. The compounds tested included 19 polycyclic aromatic hydrocarbons (PAHs), 20 PAH derivatives (nitrated, halogenated, and aminated derivatives), 23 pesticides, six amino acids, and eight amino acid metabolites. The AhR binding activities (AhR activity) of these compounds were measured using the chemical activated luciferase gene expression (CALUX) reporter gene assay system. The majority of the PAHs exhibited marked AhR activity that increased in a concentration-dependent manner. Furthermore, there was a positive link between AhR activity and the number of aromatic rings in the PAH derivatives. Conversely, there appeared to be a negative correlation between AhR activity and the number of chlorine residues present on halogenated PAH derivatives. However, there was no correlation between AhR activity and the number and position of substituents among nitrated and aminated derivatives. Among the pesticides tested, the indole-type compounds carbendazim and thiabendazole showed high levels of activity. Similarly, the indole compound tryptamine was the only amino acid metabolite to induce AhR activity. The results are useful in understanding the identification and characterization of AhR ligands in the CALUX assay.
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spelling pubmed-52245772017-02-15 Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay Amakura, Yoshiaki Tsutsumi, Tomoaki Yoshimura, Morio Nakamura, Masafumi Handa, Hiroshi Matsuda, Rieko Teshima, Reiko Watanabe, Takahiro Foods Article The purpose of this study was to examine whether a simple bioassay used for the detection of dioxins (DXNs) could be applied to detect trace amounts of harmful DXN-like substances in food products. To identify substances with possible DXN-like activity, we assessed the ability of various compounds in the environment to bind the aryl hydrocarbon receptor (AhR) that binds specifically to DXNs. The compounds tested included 19 polycyclic aromatic hydrocarbons (PAHs), 20 PAH derivatives (nitrated, halogenated, and aminated derivatives), 23 pesticides, six amino acids, and eight amino acid metabolites. The AhR binding activities (AhR activity) of these compounds were measured using the chemical activated luciferase gene expression (CALUX) reporter gene assay system. The majority of the PAHs exhibited marked AhR activity that increased in a concentration-dependent manner. Furthermore, there was a positive link between AhR activity and the number of aromatic rings in the PAH derivatives. Conversely, there appeared to be a negative correlation between AhR activity and the number of chlorine residues present on halogenated PAH derivatives. However, there was no correlation between AhR activity and the number and position of substituents among nitrated and aminated derivatives. Among the pesticides tested, the indole-type compounds carbendazim and thiabendazole showed high levels of activity. Similarly, the indole compound tryptamine was the only amino acid metabolite to induce AhR activity. The results are useful in understanding the identification and characterization of AhR ligands in the CALUX assay. MDPI 2016-02-25 /pmc/articles/PMC5224577/ /pubmed/28231110 http://dx.doi.org/10.3390/foods5010015 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Amakura, Yoshiaki
Tsutsumi, Tomoaki
Yoshimura, Morio
Nakamura, Masafumi
Handa, Hiroshi
Matsuda, Rieko
Teshima, Reiko
Watanabe, Takahiro
Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay
title Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay
title_full Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay
title_fullStr Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay
title_full_unstemmed Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay
title_short Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay
title_sort detection of aryl hydrocarbon receptor activation by some chemicals in food using a reporter gene assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224577/
https://www.ncbi.nlm.nih.gov/pubmed/28231110
http://dx.doi.org/10.3390/foods5010015
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