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Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging
Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Tr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224609/ https://www.ncbi.nlm.nih.gov/pubmed/28335258 http://dx.doi.org/10.3390/nano6070130 |
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author | Zu, Yinxue Bi, Jingran Yan, Huiping Wang, Haitao Song, Yukun Zhu, Bei-Wei Tan, Mingqian |
author_facet | Zu, Yinxue Bi, Jingran Yan, Huiping Wang, Haitao Song, Yukun Zhu, Bei-Wei Tan, Mingqian |
author_sort | Zu, Yinxue |
collection | PubMed |
description | Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Transmission electron microscopy (TEM) demonstrated that the average particle sizes are 14 nm and 75 nm for starch and chitosan NSs, respectively. Fourier transform infrared (FT-IR) spectroscopy analysis showed that there are a large number of hydroxyl or amino groups on the surface of these polysaccharide-based NSs. Strong fluorescence with an excitation-dependent emission behaviour was observed under ultraviolet excitation. Interestingly, the photostability of the NSs was found to be superior to fluorescein and rhodamine B. The quantum yield of starch NSs could reach 11.12% under the excitation of 360 nm. The oxidative metal ions including Cu(II), Hg(II)and Fe(III) exhibited a quench effect on the fluorescence intensity of the prepared NSs. Both of the two kinds of the multicoloured NSs showed a maximum fluorescence intensity at pH 7, while the fluorescence intensity decreased dramatically when they were put in an either acidic or basic environment (at pH 3 or 11). The cytotoxicity study of starch NSs showed that low cell cytotoxicity and 80% viability was found after 24 h incubation, when their concentration was less than 10 mg/mL. The study also showed the possibility of using the multicoloured starch NSs for mouse melanoma cells and guppy fish imaging. |
format | Online Article Text |
id | pubmed-5224609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-52246092017-03-21 Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging Zu, Yinxue Bi, Jingran Yan, Huiping Wang, Haitao Song, Yukun Zhu, Bei-Wei Tan, Mingqian Nanomaterials (Basel) Article Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Transmission electron microscopy (TEM) demonstrated that the average particle sizes are 14 nm and 75 nm for starch and chitosan NSs, respectively. Fourier transform infrared (FT-IR) spectroscopy analysis showed that there are a large number of hydroxyl or amino groups on the surface of these polysaccharide-based NSs. Strong fluorescence with an excitation-dependent emission behaviour was observed under ultraviolet excitation. Interestingly, the photostability of the NSs was found to be superior to fluorescein and rhodamine B. The quantum yield of starch NSs could reach 11.12% under the excitation of 360 nm. The oxidative metal ions including Cu(II), Hg(II)and Fe(III) exhibited a quench effect on the fluorescence intensity of the prepared NSs. Both of the two kinds of the multicoloured NSs showed a maximum fluorescence intensity at pH 7, while the fluorescence intensity decreased dramatically when they were put in an either acidic or basic environment (at pH 3 or 11). The cytotoxicity study of starch NSs showed that low cell cytotoxicity and 80% viability was found after 24 h incubation, when their concentration was less than 10 mg/mL. The study also showed the possibility of using the multicoloured starch NSs for mouse melanoma cells and guppy fish imaging. MDPI 2016-07-05 /pmc/articles/PMC5224609/ /pubmed/28335258 http://dx.doi.org/10.3390/nano6070130 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zu, Yinxue Bi, Jingran Yan, Huiping Wang, Haitao Song, Yukun Zhu, Bei-Wei Tan, Mingqian Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
title | Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
title_full | Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
title_fullStr | Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
title_full_unstemmed | Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
title_short | Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging |
title_sort | nanostructures derived from starch and chitosan for fluorescence bio-imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224609/ https://www.ncbi.nlm.nih.gov/pubmed/28335258 http://dx.doi.org/10.3390/nano6070130 |
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