Cargando…

Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging

Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Tr...

Descripción completa

Detalles Bibliográficos
Autores principales: Zu, Yinxue, Bi, Jingran, Yan, Huiping, Wang, Haitao, Song, Yukun, Zhu, Bei-Wei, Tan, Mingqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224609/
https://www.ncbi.nlm.nih.gov/pubmed/28335258
http://dx.doi.org/10.3390/nano6070130
_version_ 1782493392075227136
author Zu, Yinxue
Bi, Jingran
Yan, Huiping
Wang, Haitao
Song, Yukun
Zhu, Bei-Wei
Tan, Mingqian
author_facet Zu, Yinxue
Bi, Jingran
Yan, Huiping
Wang, Haitao
Song, Yukun
Zhu, Bei-Wei
Tan, Mingqian
author_sort Zu, Yinxue
collection PubMed
description Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Transmission electron microscopy (TEM) demonstrated that the average particle sizes are 14 nm and 75 nm for starch and chitosan NSs, respectively. Fourier transform infrared (FT-IR) spectroscopy analysis showed that there are a large number of hydroxyl or amino groups on the surface of these polysaccharide-based NSs. Strong fluorescence with an excitation-dependent emission behaviour was observed under ultraviolet excitation. Interestingly, the photostability of the NSs was found to be superior to fluorescein and rhodamine B. The quantum yield of starch NSs could reach 11.12% under the excitation of 360 nm. The oxidative metal ions including Cu(II), Hg(II)and Fe(III) exhibited a quench effect on the fluorescence intensity of the prepared NSs. Both of the two kinds of the multicoloured NSs showed a maximum fluorescence intensity at pH 7, while the fluorescence intensity decreased dramatically when they were put in an either acidic or basic environment (at pH 3 or 11). The cytotoxicity study of starch NSs showed that low cell cytotoxicity and 80% viability was found after 24 h incubation, when their concentration was less than 10 mg/mL. The study also showed the possibility of using the multicoloured starch NSs for mouse melanoma cells and guppy fish imaging.
format Online
Article
Text
id pubmed-5224609
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-52246092017-03-21 Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging Zu, Yinxue Bi, Jingran Yan, Huiping Wang, Haitao Song, Yukun Zhu, Bei-Wei Tan, Mingqian Nanomaterials (Basel) Article Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Transmission electron microscopy (TEM) demonstrated that the average particle sizes are 14 nm and 75 nm for starch and chitosan NSs, respectively. Fourier transform infrared (FT-IR) spectroscopy analysis showed that there are a large number of hydroxyl or amino groups on the surface of these polysaccharide-based NSs. Strong fluorescence with an excitation-dependent emission behaviour was observed under ultraviolet excitation. Interestingly, the photostability of the NSs was found to be superior to fluorescein and rhodamine B. The quantum yield of starch NSs could reach 11.12% under the excitation of 360 nm. The oxidative metal ions including Cu(II), Hg(II)and Fe(III) exhibited a quench effect on the fluorescence intensity of the prepared NSs. Both of the two kinds of the multicoloured NSs showed a maximum fluorescence intensity at pH 7, while the fluorescence intensity decreased dramatically when they were put in an either acidic or basic environment (at pH 3 or 11). The cytotoxicity study of starch NSs showed that low cell cytotoxicity and 80% viability was found after 24 h incubation, when their concentration was less than 10 mg/mL. The study also showed the possibility of using the multicoloured starch NSs for mouse melanoma cells and guppy fish imaging. MDPI 2016-07-05 /pmc/articles/PMC5224609/ /pubmed/28335258 http://dx.doi.org/10.3390/nano6070130 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zu, Yinxue
Bi, Jingran
Yan, Huiping
Wang, Haitao
Song, Yukun
Zhu, Bei-Wei
Tan, Mingqian
Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging
title Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging
title_full Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging
title_fullStr Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging
title_full_unstemmed Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging
title_short Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging
title_sort nanostructures derived from starch and chitosan for fluorescence bio-imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224609/
https://www.ncbi.nlm.nih.gov/pubmed/28335258
http://dx.doi.org/10.3390/nano6070130
work_keys_str_mv AT zuyinxue nanostructuresderivedfromstarchandchitosanforfluorescencebioimaging
AT bijingran nanostructuresderivedfromstarchandchitosanforfluorescencebioimaging
AT yanhuiping nanostructuresderivedfromstarchandchitosanforfluorescencebioimaging
AT wanghaitao nanostructuresderivedfromstarchandchitosanforfluorescencebioimaging
AT songyukun nanostructuresderivedfromstarchandchitosanforfluorescencebioimaging
AT zhubeiwei nanostructuresderivedfromstarchandchitosanforfluorescencebioimaging
AT tanmingqian nanostructuresderivedfromstarchandchitosanforfluorescencebioimaging