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DNA-Based Enzyme Reactors and Systems
During recent years, the possibility to create custom biocompatible nanoshapes using DNA as a building material has rapidly emerged. Further, these rationally designed DNA structures could be exploited in positioning pivotal molecules, such as enzymes, with nanometer-level precision. This feature co...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224616/ https://www.ncbi.nlm.nih.gov/pubmed/28335267 http://dx.doi.org/10.3390/nano6080139 |
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author | Linko, Veikko Nummelin, Sami Aarnos, Laura Tapio, Kosti Toppari, J. Jussi Kostiainen, Mauri A. |
author_facet | Linko, Veikko Nummelin, Sami Aarnos, Laura Tapio, Kosti Toppari, J. Jussi Kostiainen, Mauri A. |
author_sort | Linko, Veikko |
collection | PubMed |
description | During recent years, the possibility to create custom biocompatible nanoshapes using DNA as a building material has rapidly emerged. Further, these rationally designed DNA structures could be exploited in positioning pivotal molecules, such as enzymes, with nanometer-level precision. This feature could be used in the fabrication of artificial biochemical machinery that is able to mimic the complex reactions found in living cells. Currently, DNA-enzyme hybrids can be used to control (multi-enzyme) cascade reactions and to regulate the enzyme functions and the reaction pathways. Moreover, sophisticated DNA structures can be utilized in encapsulating active enzymes and delivering the molecular cargo into cells. In this review, we focus on the latest enzyme systems based on novel DNA nanostructures: enzyme reactors, regulatory devices and carriers that can find uses in various biotechnological and nanomedical applications. |
format | Online Article Text |
id | pubmed-5224616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-52246162017-03-21 DNA-Based Enzyme Reactors and Systems Linko, Veikko Nummelin, Sami Aarnos, Laura Tapio, Kosti Toppari, J. Jussi Kostiainen, Mauri A. Nanomaterials (Basel) Review During recent years, the possibility to create custom biocompatible nanoshapes using DNA as a building material has rapidly emerged. Further, these rationally designed DNA structures could be exploited in positioning pivotal molecules, such as enzymes, with nanometer-level precision. This feature could be used in the fabrication of artificial biochemical machinery that is able to mimic the complex reactions found in living cells. Currently, DNA-enzyme hybrids can be used to control (multi-enzyme) cascade reactions and to regulate the enzyme functions and the reaction pathways. Moreover, sophisticated DNA structures can be utilized in encapsulating active enzymes and delivering the molecular cargo into cells. In this review, we focus on the latest enzyme systems based on novel DNA nanostructures: enzyme reactors, regulatory devices and carriers that can find uses in various biotechnological and nanomedical applications. MDPI 2016-07-27 /pmc/articles/PMC5224616/ /pubmed/28335267 http://dx.doi.org/10.3390/nano6080139 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Linko, Veikko Nummelin, Sami Aarnos, Laura Tapio, Kosti Toppari, J. Jussi Kostiainen, Mauri A. DNA-Based Enzyme Reactors and Systems |
title | DNA-Based Enzyme Reactors and Systems |
title_full | DNA-Based Enzyme Reactors and Systems |
title_fullStr | DNA-Based Enzyme Reactors and Systems |
title_full_unstemmed | DNA-Based Enzyme Reactors and Systems |
title_short | DNA-Based Enzyme Reactors and Systems |
title_sort | dna-based enzyme reactors and systems |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224616/ https://www.ncbi.nlm.nih.gov/pubmed/28335267 http://dx.doi.org/10.3390/nano6080139 |
work_keys_str_mv | AT linkoveikko dnabasedenzymereactorsandsystems AT nummelinsami dnabasedenzymereactorsandsystems AT aarnoslaura dnabasedenzymereactorsandsystems AT tapiokosti dnabasedenzymereactorsandsystems AT topparijjussi dnabasedenzymereactorsandsystems AT kostiainenmauria dnabasedenzymereactorsandsystems |