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Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection
Eucalyptus leaf blight caused by Calonectria spp. is a serious disease in Eucalyptus seedling and plantations. However, the molecular mechanisms of the infection process and pathogenesis of Calonectria to Eucalyptus is not well-studied. In this study, we analyzed the transcriptomes of C. pseudoretea...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224884/ https://www.ncbi.nlm.nih.gov/pubmed/28072879 http://dx.doi.org/10.1371/journal.pone.0169598 |
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author | Ye, Xiaozhen Liu, Hongyi Jin, Yajie Guo, Mengmeng Huang, Aizhen Chen, Quanzhu Guo, Wenshuo Zhang, Feiping Feng, Lizhen |
author_facet | Ye, Xiaozhen Liu, Hongyi Jin, Yajie Guo, Mengmeng Huang, Aizhen Chen, Quanzhu Guo, Wenshuo Zhang, Feiping Feng, Lizhen |
author_sort | Ye, Xiaozhen |
collection | PubMed |
description | Eucalyptus leaf blight caused by Calonectria spp. is a serious disease in Eucalyptus seedling and plantations. However, the molecular mechanisms of the infection process and pathogenesis of Calonectria to Eucalyptus is not well-studied. In this study, we analyzed the transcriptomes of C. pseudoreteaudii at three stages of Eucalyptus leaf infection, and in mycelium grown in potato dextrose broth using Illumina RNA-Seq technology. We identified 161 differentially expressed genes between C. pseudoreteaudii from leaf and mycelium grown in potato dextrose broth. GO and KEGG enrichment analyses of these genes suggested that they were mainly involved in oxidoreductase activity, hydrolase activity, and transmembrane transporter activity. Most of the differentially expressed genes at the early infection stage were upregulated. These upregulated genes were mainly involved in cell wall hydrolysis and toxin synthesis, suggesting a role for toxin and cell wall hydrolases in the establishment of Calonectria leaf blight. Genes related to detoxification of phytoalexins were continually upregulated during infection. The candidate effectors and putative pathogenicity determinants identified in this study will help in the functional analysis of C. pseudoreteaudii virulence and pathogenicity. |
format | Online Article Text |
id | pubmed-5224884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-52248842017-01-31 Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection Ye, Xiaozhen Liu, Hongyi Jin, Yajie Guo, Mengmeng Huang, Aizhen Chen, Quanzhu Guo, Wenshuo Zhang, Feiping Feng, Lizhen PLoS One Research Article Eucalyptus leaf blight caused by Calonectria spp. is a serious disease in Eucalyptus seedling and plantations. However, the molecular mechanisms of the infection process and pathogenesis of Calonectria to Eucalyptus is not well-studied. In this study, we analyzed the transcriptomes of C. pseudoreteaudii at three stages of Eucalyptus leaf infection, and in mycelium grown in potato dextrose broth using Illumina RNA-Seq technology. We identified 161 differentially expressed genes between C. pseudoreteaudii from leaf and mycelium grown in potato dextrose broth. GO and KEGG enrichment analyses of these genes suggested that they were mainly involved in oxidoreductase activity, hydrolase activity, and transmembrane transporter activity. Most of the differentially expressed genes at the early infection stage were upregulated. These upregulated genes were mainly involved in cell wall hydrolysis and toxin synthesis, suggesting a role for toxin and cell wall hydrolases in the establishment of Calonectria leaf blight. Genes related to detoxification of phytoalexins were continually upregulated during infection. The candidate effectors and putative pathogenicity determinants identified in this study will help in the functional analysis of C. pseudoreteaudii virulence and pathogenicity. Public Library of Science 2017-01-10 /pmc/articles/PMC5224884/ /pubmed/28072879 http://dx.doi.org/10.1371/journal.pone.0169598 Text en © 2017 Ye et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ye, Xiaozhen Liu, Hongyi Jin, Yajie Guo, Mengmeng Huang, Aizhen Chen, Quanzhu Guo, Wenshuo Zhang, Feiping Feng, Lizhen Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection |
title | Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection |
title_full | Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection |
title_fullStr | Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection |
title_full_unstemmed | Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection |
title_short | Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection |
title_sort | transcriptomic analysis of calonectria pseudoreteaudii during various stages of eucalyptus infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5224884/ https://www.ncbi.nlm.nih.gov/pubmed/28072879 http://dx.doi.org/10.1371/journal.pone.0169598 |
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