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Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis
Chronic pancreatitis (CP) is an inflammatory disease characterized by progressive fibrosis of pancreas. Early diagnosis will improve the prognosis of patients. This study aimed to obtain serum miRNA biomarkers for early diagnosis of CP. In the current study, we analyzed the differentially expressed...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225423/ https://www.ncbi.nlm.nih.gov/pubmed/28074846 http://dx.doi.org/10.1038/srep40019 |
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author | Xin, Lei Gao, Jun Wang, Dan Lin, Jin-Huan Liao, Zhuan Ji, Jun-Tao Du, Ting-Ting Jiang, Fei Hu, Liang-Hao Li, Zhao-Shen |
author_facet | Xin, Lei Gao, Jun Wang, Dan Lin, Jin-Huan Liao, Zhuan Ji, Jun-Tao Du, Ting-Ting Jiang, Fei Hu, Liang-Hao Li, Zhao-Shen |
author_sort | Xin, Lei |
collection | PubMed |
description | Chronic pancreatitis (CP) is an inflammatory disease characterized by progressive fibrosis of pancreas. Early diagnosis will improve the prognosis of patients. This study aimed to obtain serum miRNA biomarkers for early diagnosis of CP. In the current study, we analyzed the differentially expressed miRNAs (DEmiRs) of CP patients from Gene Expression Omnibus (GEO), and the DEmiRs in plasma of early CP patients (n = 10) from clinic by miRNA microarrays. Expression levels of DEmiRs were further tested in clinical samples including early CP patients (n = 20), late CP patients (n = 20) and healthy controls (n = 18). The primary endpoints were area under curve (AUC) and expression levels of DEmiRs. Four DEmiRs (hsa-miR-320a-d) were obtained from GEO CP, meanwhile two (hsa-miR-221 and hsa-miR-130a) were identified as distinct biomarkers of early CP by miRNA microarrays. When applied on clinical serum samples, hsa-miR-320a-d were accurate in predicting late CP, while hsa-miR-221 and hsa-miR-130a were accurate in predicting early CP with AUC of 100.0% and 87.5%. Our study indicates that miRNA expression profile is different in early and late CP. Hsa-miR-221 and hsa-miR-130a are biomarkers of early CP, and the panel of the above 6 serum miRNAs has the potential to be applied clinically for early diagnosis of CP. |
format | Online Article Text |
id | pubmed-5225423 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52254232017-01-17 Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis Xin, Lei Gao, Jun Wang, Dan Lin, Jin-Huan Liao, Zhuan Ji, Jun-Tao Du, Ting-Ting Jiang, Fei Hu, Liang-Hao Li, Zhao-Shen Sci Rep Article Chronic pancreatitis (CP) is an inflammatory disease characterized by progressive fibrosis of pancreas. Early diagnosis will improve the prognosis of patients. This study aimed to obtain serum miRNA biomarkers for early diagnosis of CP. In the current study, we analyzed the differentially expressed miRNAs (DEmiRs) of CP patients from Gene Expression Omnibus (GEO), and the DEmiRs in plasma of early CP patients (n = 10) from clinic by miRNA microarrays. Expression levels of DEmiRs were further tested in clinical samples including early CP patients (n = 20), late CP patients (n = 20) and healthy controls (n = 18). The primary endpoints were area under curve (AUC) and expression levels of DEmiRs. Four DEmiRs (hsa-miR-320a-d) were obtained from GEO CP, meanwhile two (hsa-miR-221 and hsa-miR-130a) were identified as distinct biomarkers of early CP by miRNA microarrays. When applied on clinical serum samples, hsa-miR-320a-d were accurate in predicting late CP, while hsa-miR-221 and hsa-miR-130a were accurate in predicting early CP with AUC of 100.0% and 87.5%. Our study indicates that miRNA expression profile is different in early and late CP. Hsa-miR-221 and hsa-miR-130a are biomarkers of early CP, and the panel of the above 6 serum miRNAs has the potential to be applied clinically for early diagnosis of CP. Nature Publishing Group 2017-01-11 /pmc/articles/PMC5225423/ /pubmed/28074846 http://dx.doi.org/10.1038/srep40019 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Xin, Lei Gao, Jun Wang, Dan Lin, Jin-Huan Liao, Zhuan Ji, Jun-Tao Du, Ting-Ting Jiang, Fei Hu, Liang-Hao Li, Zhao-Shen Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis |
title | Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis |
title_full | Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis |
title_fullStr | Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis |
title_full_unstemmed | Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis |
title_short | Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis |
title_sort | novel blood-based microrna biomarker panel for early diagnosis of chronic pancreatitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225423/ https://www.ncbi.nlm.nih.gov/pubmed/28074846 http://dx.doi.org/10.1038/srep40019 |
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