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Oral treatment with Lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress
BACKGROUND: Stress-related disorders involve systemic alterations, including disruption of the intestinal microbial community. Given the putative connections between the microbiota, immunity, neural function, and behaviour, we investigated the potential for microbe-induced gut-to-brain signalling to...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225647/ https://www.ncbi.nlm.nih.gov/pubmed/28073366 http://dx.doi.org/10.1186/s12916-016-0771-7 |
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author | Bharwani, Aadil Mian, M. Firoz Surette, Michael G. Bienenstock, John Forsythe, Paul |
author_facet | Bharwani, Aadil Mian, M. Firoz Surette, Michael G. Bienenstock, John Forsythe, Paul |
author_sort | Bharwani, Aadil |
collection | PubMed |
description | BACKGROUND: Stress-related disorders involve systemic alterations, including disruption of the intestinal microbial community. Given the putative connections between the microbiota, immunity, neural function, and behaviour, we investigated the potential for microbe-induced gut-to-brain signalling to modulate the impact of stress on host behaviour and immunoregulation. METHODS: Male C57BL/6 mice treated orally over 28 days with either Lactobacillus rhamnosus (JB-1) ™ or vehicle were subjected to chronic social defeat and assessed for alterations in behaviour and immune cell phenotype. 16S rRNA sequencing and mass spectrometry were employed to analyse the faecal microbial community and metabolite profile. RESULTS: Treatment with JB-1 decreased stress-induced anxiety-like behaviour and prevented deficits in social interaction with conspecifics. However, JB-1 did not alter development of aggressor avoidance following social defeat. Microbial treatment attenuated stress-related activation of dendritic cells while increasing IL-10+ regulatory T cells. Furthermore, JB-1 modulated the effect of stress on faecal metabolites with neuroactive and immunomodulatory properties. Exposure to social defeat altered faecal microbial community composition and reduced species richness and diversity, none of which was prevented by JB-1. Stress-related microbiota disruptions persisted in vehicle-treated mice for 3 weeks following stressor cessation. CONCLUSIONS: These data demonstrate that despite the complexity of the gut microbiota, exposure to a single microbial strain can protect against certain stress-induced behaviours and systemic immune alterations without preventing dysbiosis. This work supports microbe-based interventions for stress-related disorders. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12916-016-0771-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5225647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-52256472017-01-17 Oral treatment with Lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress Bharwani, Aadil Mian, M. Firoz Surette, Michael G. Bienenstock, John Forsythe, Paul BMC Med Research Article BACKGROUND: Stress-related disorders involve systemic alterations, including disruption of the intestinal microbial community. Given the putative connections between the microbiota, immunity, neural function, and behaviour, we investigated the potential for microbe-induced gut-to-brain signalling to modulate the impact of stress on host behaviour and immunoregulation. METHODS: Male C57BL/6 mice treated orally over 28 days with either Lactobacillus rhamnosus (JB-1) ™ or vehicle were subjected to chronic social defeat and assessed for alterations in behaviour and immune cell phenotype. 16S rRNA sequencing and mass spectrometry were employed to analyse the faecal microbial community and metabolite profile. RESULTS: Treatment with JB-1 decreased stress-induced anxiety-like behaviour and prevented deficits in social interaction with conspecifics. However, JB-1 did not alter development of aggressor avoidance following social defeat. Microbial treatment attenuated stress-related activation of dendritic cells while increasing IL-10+ regulatory T cells. Furthermore, JB-1 modulated the effect of stress on faecal metabolites with neuroactive and immunomodulatory properties. Exposure to social defeat altered faecal microbial community composition and reduced species richness and diversity, none of which was prevented by JB-1. Stress-related microbiota disruptions persisted in vehicle-treated mice for 3 weeks following stressor cessation. CONCLUSIONS: These data demonstrate that despite the complexity of the gut microbiota, exposure to a single microbial strain can protect against certain stress-induced behaviours and systemic immune alterations without preventing dysbiosis. This work supports microbe-based interventions for stress-related disorders. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12916-016-0771-7) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-11 /pmc/articles/PMC5225647/ /pubmed/28073366 http://dx.doi.org/10.1186/s12916-016-0771-7 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Bharwani, Aadil Mian, M. Firoz Surette, Michael G. Bienenstock, John Forsythe, Paul Oral treatment with Lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress |
title | Oral treatment with Lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress |
title_full | Oral treatment with Lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress |
title_fullStr | Oral treatment with Lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress |
title_full_unstemmed | Oral treatment with Lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress |
title_short | Oral treatment with Lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress |
title_sort | oral treatment with lactobacillus rhamnosus attenuates behavioural deficits and immune changes in chronic social stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5225647/ https://www.ncbi.nlm.nih.gov/pubmed/28073366 http://dx.doi.org/10.1186/s12916-016-0771-7 |
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