Cargando…

PineElm_SSRdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and EST sequences of pineapple (Ananas comosus (L.) Merrill)

BACKGROUND: Simple Sequence Repeats or microsatellites are resourceful molecular genetic markers. There are only few reports of SSR identification and development in pineapple. Complete genome sequence of pineapple available in the public domain can be used to develop numerous novel SSRs. Therefore,...

Descripción completa

Detalles Bibliográficos
Autores principales: Chaudhary, Sakshi, Mishra, Bharat Kumar, Vivek, Thiruvettai, Magadum, Santoshkumar, Yasin, Jeshima Khan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226092/
https://www.ncbi.nlm.nih.gov/pubmed/28096778
http://dx.doi.org/10.1186/s41065-016-0019-8
_version_ 1782493615500558336
author Chaudhary, Sakshi
Mishra, Bharat Kumar
Vivek, Thiruvettai
Magadum, Santoshkumar
Yasin, Jeshima Khan
author_facet Chaudhary, Sakshi
Mishra, Bharat Kumar
Vivek, Thiruvettai
Magadum, Santoshkumar
Yasin, Jeshima Khan
author_sort Chaudhary, Sakshi
collection PubMed
description BACKGROUND: Simple Sequence Repeats or microsatellites are resourceful molecular genetic markers. There are only few reports of SSR identification and development in pineapple. Complete genome sequence of pineapple available in the public domain can be used to develop numerous novel SSRs. Therefore, an attempt was made to identify SSRs from genomic, chloroplast, mitochondrial and EST sequences of pineapple which will help in deciphering genetic makeup of its germplasm resources. RESULTS: A total of 359511 SSRs were identified in pineapple (356385 from genome sequence, 45 from chloroplast sequence, 249 in mitochondrial sequence and 2832 from EST sequences). The list of EST-SSR markers and their details are available in the database. CONCLUSIONS: PineElm_SSRdb is an open source database available for non-commercial academic purpose at http://app.bioelm.com/ with a mapping tool which can develop circular maps of selected marker set. This database will be of immense use to breeders, researchers and graduates working on Ananas spp. and to others working on cross-species transferability of markers, investigating diversity, mapping and DNA fingerprinting. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s41065-016-0019-8) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5226092
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-52260922017-01-17 PineElm_SSRdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and EST sequences of pineapple (Ananas comosus (L.) Merrill) Chaudhary, Sakshi Mishra, Bharat Kumar Vivek, Thiruvettai Magadum, Santoshkumar Yasin, Jeshima Khan Hereditas Research BACKGROUND: Simple Sequence Repeats or microsatellites are resourceful molecular genetic markers. There are only few reports of SSR identification and development in pineapple. Complete genome sequence of pineapple available in the public domain can be used to develop numerous novel SSRs. Therefore, an attempt was made to identify SSRs from genomic, chloroplast, mitochondrial and EST sequences of pineapple which will help in deciphering genetic makeup of its germplasm resources. RESULTS: A total of 359511 SSRs were identified in pineapple (356385 from genome sequence, 45 from chloroplast sequence, 249 in mitochondrial sequence and 2832 from EST sequences). The list of EST-SSR markers and their details are available in the database. CONCLUSIONS: PineElm_SSRdb is an open source database available for non-commercial academic purpose at http://app.bioelm.com/ with a mapping tool which can develop circular maps of selected marker set. This database will be of immense use to breeders, researchers and graduates working on Ananas spp. and to others working on cross-species transferability of markers, investigating diversity, mapping and DNA fingerprinting. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s41065-016-0019-8) contains supplementary material, which is available to authorized users. BioMed Central 2016-11-24 /pmc/articles/PMC5226092/ /pubmed/28096778 http://dx.doi.org/10.1186/s41065-016-0019-8 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Chaudhary, Sakshi
Mishra, Bharat Kumar
Vivek, Thiruvettai
Magadum, Santoshkumar
Yasin, Jeshima Khan
PineElm_SSRdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and EST sequences of pineapple (Ananas comosus (L.) Merrill)
title PineElm_SSRdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and EST sequences of pineapple (Ananas comosus (L.) Merrill)
title_full PineElm_SSRdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and EST sequences of pineapple (Ananas comosus (L.) Merrill)
title_fullStr PineElm_SSRdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and EST sequences of pineapple (Ananas comosus (L.) Merrill)
title_full_unstemmed PineElm_SSRdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and EST sequences of pineapple (Ananas comosus (L.) Merrill)
title_short PineElm_SSRdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and EST sequences of pineapple (Ananas comosus (L.) Merrill)
title_sort pineelm_ssrdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and est sequences of pineapple (ananas comosus (l.) merrill)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226092/
https://www.ncbi.nlm.nih.gov/pubmed/28096778
http://dx.doi.org/10.1186/s41065-016-0019-8
work_keys_str_mv AT chaudharysakshi pineelmssrdbamicrosatellitemarkerdatabaseidentifiedfromgenomicchloroplastmitochondrialandestsequencesofpineappleananascomosuslmerrill
AT mishrabharatkumar pineelmssrdbamicrosatellitemarkerdatabaseidentifiedfromgenomicchloroplastmitochondrialandestsequencesofpineappleananascomosuslmerrill
AT vivekthiruvettai pineelmssrdbamicrosatellitemarkerdatabaseidentifiedfromgenomicchloroplastmitochondrialandestsequencesofpineappleananascomosuslmerrill
AT magadumsantoshkumar pineelmssrdbamicrosatellitemarkerdatabaseidentifiedfromgenomicchloroplastmitochondrialandestsequencesofpineappleananascomosuslmerrill
AT yasinjeshimakhan pineelmssrdbamicrosatellitemarkerdatabaseidentifiedfromgenomicchloroplastmitochondrialandestsequencesofpineappleananascomosuslmerrill