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Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
The capacity to introduce exogenous proteins and express (or down-regulate) specific genes in plants provides a powerful tool for fundamental research as well as new applications in the field of plant biotechnology. Viable methods that currently exist for protein or gene transfer into plant cells, n...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226412/ https://www.ncbi.nlm.nih.gov/pubmed/28060264 http://dx.doi.org/10.3791/54972 |
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author | Chuah, Jo-Ann Horii, Yoko Numata, Keiji |
author_facet | Chuah, Jo-Ann Horii, Yoko Numata, Keiji |
author_sort | Chuah, Jo-Ann |
collection | PubMed |
description | The capacity to introduce exogenous proteins and express (or down-regulate) specific genes in plants provides a powerful tool for fundamental research as well as new applications in the field of plant biotechnology. Viable methods that currently exist for protein or gene transfer into plant cells, namely Agrobacterium and microprojectile bombardment, have disadvantages of low transformation frequency, limited host range, or a high cost of equipment and microcarriers. The following protocol outlines a simple and versatile method, which employs rationally-designed peptides as delivery agents for a variety of nucleic acid- and protein-based cargoes into plants. Peptides are selected as tools for development of the system due to their biodegradability, reduced size, diverse and tunable properties as well as the ability to gain intracellular/organellar access. The preparation, characterization and application of optimized formulations for each type of the wide range of delivered cargoes (plasmid DNA, double-stranded DNA or RNA, and protein) are described. Critical steps within the protocol, possible modifications and existing limitations of the method are also discussed. |
format | Online Article Text |
id | pubmed-5226412 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-52264122017-01-26 Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants Chuah, Jo-Ann Horii, Yoko Numata, Keiji J Vis Exp Genetics The capacity to introduce exogenous proteins and express (or down-regulate) specific genes in plants provides a powerful tool for fundamental research as well as new applications in the field of plant biotechnology. Viable methods that currently exist for protein or gene transfer into plant cells, namely Agrobacterium and microprojectile bombardment, have disadvantages of low transformation frequency, limited host range, or a high cost of equipment and microcarriers. The following protocol outlines a simple and versatile method, which employs rationally-designed peptides as delivery agents for a variety of nucleic acid- and protein-based cargoes into plants. Peptides are selected as tools for development of the system due to their biodegradability, reduced size, diverse and tunable properties as well as the ability to gain intracellular/organellar access. The preparation, characterization and application of optimized formulations for each type of the wide range of delivered cargoes (plasmid DNA, double-stranded DNA or RNA, and protein) are described. Critical steps within the protocol, possible modifications and existing limitations of the method are also discussed. MyJove Corporation 2016-12-16 /pmc/articles/PMC5226412/ /pubmed/28060264 http://dx.doi.org/10.3791/54972 Text en Copyright © 2016, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Genetics Chuah, Jo-Ann Horii, Yoko Numata, Keiji Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants |
title | Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants |
title_full | Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants |
title_fullStr | Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants |
title_full_unstemmed | Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants |
title_short | Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants |
title_sort | peptide-derived method to transport genes and proteins across cellular and organellar barriers in plants |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226412/ https://www.ncbi.nlm.nih.gov/pubmed/28060264 http://dx.doi.org/10.3791/54972 |
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