Cargando…

Optimization of a Quantitative Micro-neutralization Assay

The micro-neutralization (MN) assay is a standard technique for measuring the infectivity of the influenza virus and the inhibition of virus replication. In this study, we present the protocol of an imaging-based MN assay to quantify the true antigenic relationships between viruses. Unlike typical p...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lin, Yipu, Gu, Yan, McCauley, John W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2016
Materias:	Infection
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226418/ https://www.ncbi.nlm.nih.gov/pubmed/28060291 http://dx.doi.org/10.3791/54897

Ejemplares similares

Optimisation of a micro-neutralisation assay and its application in antigenic characterisation of influenza viruses
por: Lin, Yipu, et al.
Publicado: (2015)

Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria
por: Gold, Ben, et al.
Publicado: (2016)

Characterization of neutralizing epitopes in antigenic site B of recently circulating influenza A(H3N2) viruses
por: Beer, Kerstin, et al.
Publicado: (2018)

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
por: Cheung, Ruby, et al.
Publicado: (2018)

Comparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient Samples
por: Vial, Cecilia, et al.
Publicado: (2020)

A Semi-Quantitative Assay to Measure Glycosaminoglycan Degradation by the Urinary Microbiota
por: Nguyen, Vivian H., et al.
Publicado: (2022)

Effects of egg-adaptation on receptor-binding and antigenic properties of recent influenza A (H3N2) vaccine viruses
por: Parker, Lauren, et al.
Publicado: (2016)

A Visual Assay to Monitor T6SS-mediated Bacterial Competition
por: Hachani, Abderrahman, et al.
Publicado: (2013)

The instantly blocking-based fluorescent immunochromatographic assay for the detection of SARS-CoV-2 neutralizing antibody
por: Li, Yizhe, et al.
Publicado: (2023)

Electrochemiluminescence Assays for Human Islet Autoantibodies
por: Gu, Yong, et al.
Publicado: (2018)

Implementation of Microfluidics for Antimicrobial Susceptibility Assays: Issues and Optimization Requirements
por: Parsley, Nicole C., et al.
Publicado: (2020)

Semi-Quantitative Assay to Measure Urease Activity by Urinary Catheter-Associated Uropathogens
por: Duran Ramirez, Jesus M., et al.
Publicado: (2022)

Akkermansia muciniphila as a Model Case for the Development of an Improved Quantitative RPA Microbiome Assay
por: Goux, Heather J., et al.
Publicado: (2018)

Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19
por: Tian, Linhua, et al.
Publicado: (2021)

Corrigendum: Akkermansia muciniphila as a Model Case for the Development of an Improved Quantitative RPA Microbiome Assay
por: Goux, Heather J., et al.
Publicado: (2019)

Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays
por: Lee, Beomki, et al.
Publicado: (2022)

Evaluation of two rapid commercial assays for detection of Streptococcus agalactiae from vaginal samples
por: Koliwer‐Brandl, Hendrik, et al.
Publicado: (2023)

Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
por: Barkway, Christopher P., et al.
Publicado: (2015)

The characteristics and antigenic properties of recently emerged subclade 3C.3a and 3C.2a human influenza A(H3N2) viruses passaged in MDCK cells
por: Lin, Yipu, et al.
Publicado: (2017)

Optimal concentration of ethylenediaminetetraacetic acid as an irrigation solution additive to reduce infection rates in rat models of contaminated wound
por: Lin, Junqing, et al.
Publicado: (2021)

Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay
por: Tolah, Ahmed Majdi K., et al.
Publicado: (2021)

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
por: Bollampalli, Vishnu Priya, et al.
Publicado: (2016)

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
por: Jain, Surendra K., et al.
Publicado: (2012)

Calibration of an Upconverting Phosphor-Based Quantitative Immunochromatographic Assay for Detecting Yersinia pestis, Brucella spp., and Bacillus anthracis Spores
por: Zhang, Pingping, et al.
Publicado: (2020)

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery
por: Veneman, Wouter J., et al.
Publicado: (2014)

Ocular Effects of Glycyrrhizin at Acidic and Neutral pH
por: Somayajulu, Mallika, et al.
Publicado: (2022)

Evidence of Neutralizing and Non-Neutralizing Anti-Glucosaminidase Antibodies in Patients With S. Aureus Osteomyelitis and Their Association With Clinical Outcome Following Surgery in a Clinical Pilot
por: Sherchand, Shardulendra Prasad, et al.
Publicado: (2022)

A rapid and quantitative assay for measuring neutralizing antibodies of Coxsackievirus B3
por: Chen, Pan, et al.
Publicado: (2016)

Persistence of neutralizing antibodies a year after SARS‐CoV‐2 infection in humans
por: Haveri, Anu, et al.
Publicado: (2021)

A quantitative assay for detection of SARS-CoV-2 neutralizing antibodies
por: Cerutti, Helena, et al.
Publicado: (2022)

Repeated exposure to dengue virus elicits robust cross neutralizing antibodies against Zika virus in residents of Northeastern Thailand
por: Hattakam, Sararat, et al.
Publicado: (2021)

Timely identification of optimal control strategies for emerging infectious diseases()
por: Feng, Zhilan, et al.
Publicado: (2009)

Global Pandemic Preparedness: Optimizing Our Capabilities and the Influenza Experience
por: Rockman, Steven, et al.
Publicado: (2022)

Development, Optimization, and Validation of a High Throughput Screening Assay for Identification of Tat and Type II Secretion Inhibitors of Pseudomonas aeruginosa
por: Massai, Francesco, et al.
Publicado: (2019)

Reverse Transcription Recombinase-Aided Amplification Assay With Lateral Flow Dipstick Assay for Rapid Detection of 2019 Novel Coronavirus
por: Zheng, Yu-Zhong, et al.
Publicado: (2021)

Augmented neutralization of SARS‐CoV‐2 Omicron variant by boost vaccination and monoclonal antibodies
por: Schulz, Sebastian R., et al.
Publicado: (2022)

Teaching Neuroinformatics with an Emphasis on Quantitative Locus Analysis
por: Grisham, William, et al.
Publicado: (2012)

Dual Recognition Element Lateral Flow Assay Toward Multiplex Strain Specific Influenza Virus Detection
por: Le, Thao T., et al.
Publicado: (2017)

A pair of noncompeting neutralizing human monoclonal antibodies protecting from disease in a SARS‐CoV‐2 infection model
por: Peter, Antonia Sophia, et al.
Publicado: (2021)

Broadly Neutralizing Antibodies to SARS-CoV-2 Provide Novel Insights Into the Neutralization of Variants and Other Human Coronaviruses
por: Bajpai, Prashant, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_r7ob9cbue4cm5j0ugfh177oiam