Cargando…

High intensity focused ultrasound inhibits melanoma cell migration and metastasis through attenuating microRNA-21-mediated PTEN suppression

High intensity focused ultrasound (HIFU) technology is becoming a potential noninvasive treatment for solid tumor. To explore whether HIFU can be applied to treat melanoma and its metastasis, we investigated the effect of HIFU on murine melanoma model. While there was little influence on cell surviv...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Huan, Yuan, Shi-mei, Yang, Min, Zha, He, Li, Xue-ru, Sun, Hui, Duan, Liang, Gu, Yue, Li, Ai-fang, Weng, Ya-guang, Luo, Jin-yong, He, Tong-chuan, Wang, Yan, Li, Chong-yan, Li, Fa-qi, Wang, Zhi-biao, Zhou, Lan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226595/
https://www.ncbi.nlm.nih.gov/pubmed/27391071
http://dx.doi.org/10.18632/oncotarget.10433
_version_ 1782493674668556288
author Li, Huan
Yuan, Shi-mei
Yang, Min
Zha, He
Li, Xue-ru
Sun, Hui
Duan, Liang
Gu, Yue
Li, Ai-fang
Weng, Ya-guang
Luo, Jin-yong
He, Tong-chuan
Wang, Yan
Li, Chong-yan
Li, Fa-qi
Wang, Zhi-biao
Zhou, Lan
author_facet Li, Huan
Yuan, Shi-mei
Yang, Min
Zha, He
Li, Xue-ru
Sun, Hui
Duan, Liang
Gu, Yue
Li, Ai-fang
Weng, Ya-guang
Luo, Jin-yong
He, Tong-chuan
Wang, Yan
Li, Chong-yan
Li, Fa-qi
Wang, Zhi-biao
Zhou, Lan
author_sort Li, Huan
collection PubMed
description High intensity focused ultrasound (HIFU) technology is becoming a potential noninvasive treatment for solid tumor. To explore whether HIFU can be applied to treat melanoma and its metastasis, we investigated the effect of HIFU on murine melanoma model. While there was little influence on cell survival, viability or apoptosis, HIFU exposure suppressed melanoma cell migration in vitro and metastasis in vivo. The expression of microRNA-21(miR-21) was down-regulated and PTEN expression was up-regulated in response to HIFU exposure, which was in concomitant with the reduction of AKT activity. Furthermore, ectopic miR-21 expression suppressed this effect of HIFU. These results demonstrate that HIFU exposure can inhibit AKT-mediated melanoma metastasis via miR-21 inhibition to restore PTEN expression. Therefore, targeting the miR-21/PTEN/AKT pathway might be a novel strategy of HIFU in treatment of melanoma.
format Online
Article
Text
id pubmed-5226595
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-52265952017-01-18 High intensity focused ultrasound inhibits melanoma cell migration and metastasis through attenuating microRNA-21-mediated PTEN suppression Li, Huan Yuan, Shi-mei Yang, Min Zha, He Li, Xue-ru Sun, Hui Duan, Liang Gu, Yue Li, Ai-fang Weng, Ya-guang Luo, Jin-yong He, Tong-chuan Wang, Yan Li, Chong-yan Li, Fa-qi Wang, Zhi-biao Zhou, Lan Oncotarget Research Paper High intensity focused ultrasound (HIFU) technology is becoming a potential noninvasive treatment for solid tumor. To explore whether HIFU can be applied to treat melanoma and its metastasis, we investigated the effect of HIFU on murine melanoma model. While there was little influence on cell survival, viability or apoptosis, HIFU exposure suppressed melanoma cell migration in vitro and metastasis in vivo. The expression of microRNA-21(miR-21) was down-regulated and PTEN expression was up-regulated in response to HIFU exposure, which was in concomitant with the reduction of AKT activity. Furthermore, ectopic miR-21 expression suppressed this effect of HIFU. These results demonstrate that HIFU exposure can inhibit AKT-mediated melanoma metastasis via miR-21 inhibition to restore PTEN expression. Therefore, targeting the miR-21/PTEN/AKT pathway might be a novel strategy of HIFU in treatment of melanoma. Impact Journals LLC 2016-07-06 /pmc/articles/PMC5226595/ /pubmed/27391071 http://dx.doi.org/10.18632/oncotarget.10433 Text en Copyright: © 2016 Li et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Li, Huan
Yuan, Shi-mei
Yang, Min
Zha, He
Li, Xue-ru
Sun, Hui
Duan, Liang
Gu, Yue
Li, Ai-fang
Weng, Ya-guang
Luo, Jin-yong
He, Tong-chuan
Wang, Yan
Li, Chong-yan
Li, Fa-qi
Wang, Zhi-biao
Zhou, Lan
High intensity focused ultrasound inhibits melanoma cell migration and metastasis through attenuating microRNA-21-mediated PTEN suppression
title High intensity focused ultrasound inhibits melanoma cell migration and metastasis through attenuating microRNA-21-mediated PTEN suppression
title_full High intensity focused ultrasound inhibits melanoma cell migration and metastasis through attenuating microRNA-21-mediated PTEN suppression
title_fullStr High intensity focused ultrasound inhibits melanoma cell migration and metastasis through attenuating microRNA-21-mediated PTEN suppression
title_full_unstemmed High intensity focused ultrasound inhibits melanoma cell migration and metastasis through attenuating microRNA-21-mediated PTEN suppression
title_short High intensity focused ultrasound inhibits melanoma cell migration and metastasis through attenuating microRNA-21-mediated PTEN suppression
title_sort high intensity focused ultrasound inhibits melanoma cell migration and metastasis through attenuating microrna-21-mediated pten suppression
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226595/
https://www.ncbi.nlm.nih.gov/pubmed/27391071
http://dx.doi.org/10.18632/oncotarget.10433
work_keys_str_mv AT lihuan highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT yuanshimei highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT yangmin highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT zhahe highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT lixueru highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT sunhui highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT duanliang highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT guyue highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT liaifang highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT wengyaguang highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT luojinyong highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT hetongchuan highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT wangyan highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT lichongyan highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT lifaqi highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT wangzhibiao highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression
AT zhoulan highintensityfocusedultrasoundinhibitsmelanomacellmigrationandmetastasisthroughattenuatingmicrorna21mediatedptensuppression