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TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology

Whether a mixed type of craniopharyngioma (CP) exists and whether papillary craniopharyngioma (pCP) is on a histopathological continuum with Rathke's cleft cyst (RCC) remain controversial. Herein, we examined the expression and localization of β-catenin, BRAF p.V600E (V600E), and triggering rec...

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Autores principales: Liu, Yi, Wang, Chao-hu, Li, Dan-ling, Zhang, Shi-chao, Peng, Yu-ping, Peng, Jun-xiang, Song, Ye, Qi, Song-tao, Pan, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226603/
https://www.ncbi.nlm.nih.gov/pubmed/27409178
http://dx.doi.org/10.18632/oncotarget.10501
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author Liu, Yi
Wang, Chao-hu
Li, Dan-ling
Zhang, Shi-chao
Peng, Yu-ping
Peng, Jun-xiang
Song, Ye
Qi, Song-tao
Pan, Jun
author_facet Liu, Yi
Wang, Chao-hu
Li, Dan-ling
Zhang, Shi-chao
Peng, Yu-ping
Peng, Jun-xiang
Song, Ye
Qi, Song-tao
Pan, Jun
author_sort Liu, Yi
collection PubMed
description Whether a mixed type of craniopharyngioma (CP) exists and whether papillary craniopharyngioma (pCP) is on a histopathological continuum with Rathke's cleft cyst (RCC) remain controversial. Herein, we examined the expression and localization of β-catenin, BRAF p.V600E (V600E), and triggering receptor expressed on myeloid cells-1 (TREM-1) in 58 samples including 20 pCPs, 26 adamantinomatous craniopharyngiomas (aCP), and 12 RCCs. Five aCPs were diagnosed with mixed type CPs and the remaining 21 cases were pure aCPs. Four of the 12 RCCs presented with significant squamous epithelium (SE). V600E immunoreactivity was observed in all pCPs in the cytoplasm, but not in the nuclei. aCPs and RCCs, including mixed type CP, did not express V600E. Nuclear β-catenin translocation was detected exclusively in aCPs. TREM-1 was expressed in pCPs. Additionally, TREM-1 expression was detected in the SE of 5 “mixed type” CPs, while it was absent in pure aCPs. TREM-1 was expressed in 4 RCCs with SE, but not in the remaining 8 RCCs. TREM-1 mRNA levels were compared in cultured pCP and aCP cells. TREM-1 mRNA level was significantly (p < 0.001; up to 4.045 fold) higher in pCPs than in aCPs. Western blotting revealed a significantly (p < 0.001; up to 7.19 fold) lower level of TREM-1 expression in aCP cells compared to that in pCP cells. Our findings further supported that RCC and pCP may represent two ends of a morphological spectrum. A variant showing overlapping histological features of aCP and pCP should not be considered as a mixed type.
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spelling pubmed-52266032017-01-18 TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology Liu, Yi Wang, Chao-hu Li, Dan-ling Zhang, Shi-chao Peng, Yu-ping Peng, Jun-xiang Song, Ye Qi, Song-tao Pan, Jun Oncotarget Research Paper Whether a mixed type of craniopharyngioma (CP) exists and whether papillary craniopharyngioma (pCP) is on a histopathological continuum with Rathke's cleft cyst (RCC) remain controversial. Herein, we examined the expression and localization of β-catenin, BRAF p.V600E (V600E), and triggering receptor expressed on myeloid cells-1 (TREM-1) in 58 samples including 20 pCPs, 26 adamantinomatous craniopharyngiomas (aCP), and 12 RCCs. Five aCPs were diagnosed with mixed type CPs and the remaining 21 cases were pure aCPs. Four of the 12 RCCs presented with significant squamous epithelium (SE). V600E immunoreactivity was observed in all pCPs in the cytoplasm, but not in the nuclei. aCPs and RCCs, including mixed type CP, did not express V600E. Nuclear β-catenin translocation was detected exclusively in aCPs. TREM-1 was expressed in pCPs. Additionally, TREM-1 expression was detected in the SE of 5 “mixed type” CPs, while it was absent in pure aCPs. TREM-1 was expressed in 4 RCCs with SE, but not in the remaining 8 RCCs. TREM-1 mRNA levels were compared in cultured pCP and aCP cells. TREM-1 mRNA level was significantly (p < 0.001; up to 4.045 fold) higher in pCPs than in aCPs. Western blotting revealed a significantly (p < 0.001; up to 7.19 fold) lower level of TREM-1 expression in aCP cells compared to that in pCP cells. Our findings further supported that RCC and pCP may represent two ends of a morphological spectrum. A variant showing overlapping histological features of aCP and pCP should not be considered as a mixed type. Impact Journals LLC 2016-07-08 /pmc/articles/PMC5226603/ /pubmed/27409178 http://dx.doi.org/10.18632/oncotarget.10501 Text en Copyright: © 2016 Liu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Liu, Yi
Wang, Chao-hu
Li, Dan-ling
Zhang, Shi-chao
Peng, Yu-ping
Peng, Jun-xiang
Song, Ye
Qi, Song-tao
Pan, Jun
TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology
title TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology
title_full TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology
title_fullStr TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology
title_full_unstemmed TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology
title_short TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology
title_sort trem-1 expression in craniopharyngioma and rathke's cleft cyst: its possible implication for controversial pathology
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226603/
https://www.ncbi.nlm.nih.gov/pubmed/27409178
http://dx.doi.org/10.18632/oncotarget.10501
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