Cargando…
TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology
Whether a mixed type of craniopharyngioma (CP) exists and whether papillary craniopharyngioma (pCP) is on a histopathological continuum with Rathke's cleft cyst (RCC) remain controversial. Herein, we examined the expression and localization of β-catenin, BRAF p.V600E (V600E), and triggering rec...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226603/ https://www.ncbi.nlm.nih.gov/pubmed/27409178 http://dx.doi.org/10.18632/oncotarget.10501 |
_version_ | 1782493676493078528 |
---|---|
author | Liu, Yi Wang, Chao-hu Li, Dan-ling Zhang, Shi-chao Peng, Yu-ping Peng, Jun-xiang Song, Ye Qi, Song-tao Pan, Jun |
author_facet | Liu, Yi Wang, Chao-hu Li, Dan-ling Zhang, Shi-chao Peng, Yu-ping Peng, Jun-xiang Song, Ye Qi, Song-tao Pan, Jun |
author_sort | Liu, Yi |
collection | PubMed |
description | Whether a mixed type of craniopharyngioma (CP) exists and whether papillary craniopharyngioma (pCP) is on a histopathological continuum with Rathke's cleft cyst (RCC) remain controversial. Herein, we examined the expression and localization of β-catenin, BRAF p.V600E (V600E), and triggering receptor expressed on myeloid cells-1 (TREM-1) in 58 samples including 20 pCPs, 26 adamantinomatous craniopharyngiomas (aCP), and 12 RCCs. Five aCPs were diagnosed with mixed type CPs and the remaining 21 cases were pure aCPs. Four of the 12 RCCs presented with significant squamous epithelium (SE). V600E immunoreactivity was observed in all pCPs in the cytoplasm, but not in the nuclei. aCPs and RCCs, including mixed type CP, did not express V600E. Nuclear β-catenin translocation was detected exclusively in aCPs. TREM-1 was expressed in pCPs. Additionally, TREM-1 expression was detected in the SE of 5 “mixed type” CPs, while it was absent in pure aCPs. TREM-1 was expressed in 4 RCCs with SE, but not in the remaining 8 RCCs. TREM-1 mRNA levels were compared in cultured pCP and aCP cells. TREM-1 mRNA level was significantly (p < 0.001; up to 4.045 fold) higher in pCPs than in aCPs. Western blotting revealed a significantly (p < 0.001; up to 7.19 fold) lower level of TREM-1 expression in aCP cells compared to that in pCP cells. Our findings further supported that RCC and pCP may represent two ends of a morphological spectrum. A variant showing overlapping histological features of aCP and pCP should not be considered as a mixed type. |
format | Online Article Text |
id | pubmed-5226603 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-52266032017-01-18 TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology Liu, Yi Wang, Chao-hu Li, Dan-ling Zhang, Shi-chao Peng, Yu-ping Peng, Jun-xiang Song, Ye Qi, Song-tao Pan, Jun Oncotarget Research Paper Whether a mixed type of craniopharyngioma (CP) exists and whether papillary craniopharyngioma (pCP) is on a histopathological continuum with Rathke's cleft cyst (RCC) remain controversial. Herein, we examined the expression and localization of β-catenin, BRAF p.V600E (V600E), and triggering receptor expressed on myeloid cells-1 (TREM-1) in 58 samples including 20 pCPs, 26 adamantinomatous craniopharyngiomas (aCP), and 12 RCCs. Five aCPs were diagnosed with mixed type CPs and the remaining 21 cases were pure aCPs. Four of the 12 RCCs presented with significant squamous epithelium (SE). V600E immunoreactivity was observed in all pCPs in the cytoplasm, but not in the nuclei. aCPs and RCCs, including mixed type CP, did not express V600E. Nuclear β-catenin translocation was detected exclusively in aCPs. TREM-1 was expressed in pCPs. Additionally, TREM-1 expression was detected in the SE of 5 “mixed type” CPs, while it was absent in pure aCPs. TREM-1 was expressed in 4 RCCs with SE, but not in the remaining 8 RCCs. TREM-1 mRNA levels were compared in cultured pCP and aCP cells. TREM-1 mRNA level was significantly (p < 0.001; up to 4.045 fold) higher in pCPs than in aCPs. Western blotting revealed a significantly (p < 0.001; up to 7.19 fold) lower level of TREM-1 expression in aCP cells compared to that in pCP cells. Our findings further supported that RCC and pCP may represent two ends of a morphological spectrum. A variant showing overlapping histological features of aCP and pCP should not be considered as a mixed type. Impact Journals LLC 2016-07-08 /pmc/articles/PMC5226603/ /pubmed/27409178 http://dx.doi.org/10.18632/oncotarget.10501 Text en Copyright: © 2016 Liu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Liu, Yi Wang, Chao-hu Li, Dan-ling Zhang, Shi-chao Peng, Yu-ping Peng, Jun-xiang Song, Ye Qi, Song-tao Pan, Jun TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology |
title | TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology |
title_full | TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology |
title_fullStr | TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology |
title_full_unstemmed | TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology |
title_short | TREM-1 expression in craniopharyngioma and Rathke's cleft cyst: its possible implication for controversial pathology |
title_sort | trem-1 expression in craniopharyngioma and rathke's cleft cyst: its possible implication for controversial pathology |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226603/ https://www.ncbi.nlm.nih.gov/pubmed/27409178 http://dx.doi.org/10.18632/oncotarget.10501 |
work_keys_str_mv | AT liuyi trem1expressionincraniopharyngiomaandrathkescleftcystitspossibleimplicationforcontroversialpathology AT wangchaohu trem1expressionincraniopharyngiomaandrathkescleftcystitspossibleimplicationforcontroversialpathology AT lidanling trem1expressionincraniopharyngiomaandrathkescleftcystitspossibleimplicationforcontroversialpathology AT zhangshichao trem1expressionincraniopharyngiomaandrathkescleftcystitspossibleimplicationforcontroversialpathology AT pengyuping trem1expressionincraniopharyngiomaandrathkescleftcystitspossibleimplicationforcontroversialpathology AT pengjunxiang trem1expressionincraniopharyngiomaandrathkescleftcystitspossibleimplicationforcontroversialpathology AT songye trem1expressionincraniopharyngiomaandrathkescleftcystitspossibleimplicationforcontroversialpathology AT qisongtao trem1expressionincraniopharyngiomaandrathkescleftcystitspossibleimplicationforcontroversialpathology AT panjun trem1expressionincraniopharyngiomaandrathkescleftcystitspossibleimplicationforcontroversialpathology |