Development and Validation of a New Reliable Method for the Diagnosis of Avian Botulism

Liver is a reliable matrix for laboratory confirmation of avian botulism using real-time PCR. Here, we developed, optimized, and validated the analytical steps preceding PCR to maximize the detection of Clostridium botulinum group III in avian liver. These pre-PCR steps included enrichment incubatio...

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Detalles Bibliográficos
Autores principales: Le Maréchal, Caroline, Rouxel, Sandra, Ballan, Valentine, Houard, Emmanuelle, Poezevara, Typhaine, Bayon-Auboyer, Marie-Hélène, Souillard, Rozenn, Morvan, Hervé, Baudouard, Marie-Agnès, Woudstra, Cédric, Mazuet, Christelle, Le Bouquin, Sophie, Fach, Patrick, Popoff, Michel, Chemaly, Marianne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226734/
https://www.ncbi.nlm.nih.gov/pubmed/28076405
http://dx.doi.org/10.1371/journal.pone.0169640

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226734/
https://www.ncbi.nlm.nih.gov/pubmed/28076405
http://dx.doi.org/10.1371/journal.pone.0169640

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