Cargando…
Genetic Structure and Drug Susceptibility Patterns of Mycobacterium tuberculosis Complex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon
Background. Cameroon this last decade continues to present a low contribution of M. africanum and M. bovis in human tuberculosis (TB), while M. bovis was prevalent in cattle but all these pieces of information only concerned West and Center regions. Methods. We carried out the first study in Adamaou...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5227118/ https://www.ncbi.nlm.nih.gov/pubmed/28119925 http://dx.doi.org/10.1155/2016/2904832 |
_version_ | 1782493765308514304 |
---|---|
author | Koro Koro, Francioli Um Boock, Alphonse Kaiyven, Afi Leslie Noeske, Juergen Gutierrez, Cristina Kuaban, Christopher Etoa, François-Xavier Eyangoh, Sara Irène |
author_facet | Koro Koro, Francioli Um Boock, Alphonse Kaiyven, Afi Leslie Noeske, Juergen Gutierrez, Cristina Kuaban, Christopher Etoa, François-Xavier Eyangoh, Sara Irène |
author_sort | Koro Koro, Francioli |
collection | PubMed |
description | Background. Cameroon this last decade continues to present a low contribution of M. africanum and M. bovis in human tuberculosis (TB), while M. bovis was prevalent in cattle but all these pieces of information only concerned West and Center regions. Methods. We carried out the first study in Adamaoua, one of the most rearing regions of Cameroon, on the genetic structure and drug susceptibility of the MTBC strains isolated from newly diagnosed sputum smear-positive patients aged 15 years and above. For that purpose, spoligotyping, a modified 15 standard MIRU/VNTR loci typing, and the proportion method were used. Results. Four hundred and thirty-seven MTBC isolates were analyzed by spoligotyping. Of these, 423 were identified as M. tuberculosis, within the Cameroon family being dominant with 278 (65.7%) isolates; twelve (2.75%) isolates were classified as M. africanum and two as M. bovis. MIRU/VNTR typing of the most prevalent sublineage (SIT 61) suggested that this lineage is not a unique clone as thought earlier but could constitute a group of strains implicated to different pocket of TB transmission. Only M. tuberculosis sublineages were associated with antituberculosis drug resistance. Conclusion. These results showed the weak contribution of M. africanum and M. bovis to human active pulmonary tuberculosis in Cameroon even in the rearing region. |
format | Online Article Text |
id | pubmed-5227118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-52271182017-01-24 Genetic Structure and Drug Susceptibility Patterns of Mycobacterium tuberculosis Complex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon Koro Koro, Francioli Um Boock, Alphonse Kaiyven, Afi Leslie Noeske, Juergen Gutierrez, Cristina Kuaban, Christopher Etoa, François-Xavier Eyangoh, Sara Irène Biomed Res Int Research Article Background. Cameroon this last decade continues to present a low contribution of M. africanum and M. bovis in human tuberculosis (TB), while M. bovis was prevalent in cattle but all these pieces of information only concerned West and Center regions. Methods. We carried out the first study in Adamaoua, one of the most rearing regions of Cameroon, on the genetic structure and drug susceptibility of the MTBC strains isolated from newly diagnosed sputum smear-positive patients aged 15 years and above. For that purpose, spoligotyping, a modified 15 standard MIRU/VNTR loci typing, and the proportion method were used. Results. Four hundred and thirty-seven MTBC isolates were analyzed by spoligotyping. Of these, 423 were identified as M. tuberculosis, within the Cameroon family being dominant with 278 (65.7%) isolates; twelve (2.75%) isolates were classified as M. africanum and two as M. bovis. MIRU/VNTR typing of the most prevalent sublineage (SIT 61) suggested that this lineage is not a unique clone as thought earlier but could constitute a group of strains implicated to different pocket of TB transmission. Only M. tuberculosis sublineages were associated with antituberculosis drug resistance. Conclusion. These results showed the weak contribution of M. africanum and M. bovis to human active pulmonary tuberculosis in Cameroon even in the rearing region. Hindawi Publishing Corporation 2016 2016-12-29 /pmc/articles/PMC5227118/ /pubmed/28119925 http://dx.doi.org/10.1155/2016/2904832 Text en |
spellingShingle | Research Article Koro Koro, Francioli Um Boock, Alphonse Kaiyven, Afi Leslie Noeske, Juergen Gutierrez, Cristina Kuaban, Christopher Etoa, François-Xavier Eyangoh, Sara Irène Genetic Structure and Drug Susceptibility Patterns of Mycobacterium tuberculosis Complex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon |
title | Genetic Structure and Drug Susceptibility Patterns of Mycobacterium tuberculosis Complex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon |
title_full | Genetic Structure and Drug Susceptibility Patterns of Mycobacterium tuberculosis Complex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon |
title_fullStr | Genetic Structure and Drug Susceptibility Patterns of Mycobacterium tuberculosis Complex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon |
title_full_unstemmed | Genetic Structure and Drug Susceptibility Patterns of Mycobacterium tuberculosis Complex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon |
title_short | Genetic Structure and Drug Susceptibility Patterns of Mycobacterium tuberculosis Complex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon |
title_sort | genetic structure and drug susceptibility patterns of mycobacterium tuberculosis complex strains responsible of human pulmonary tuberculosis in the major rearing region in cameroon |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5227118/ https://www.ncbi.nlm.nih.gov/pubmed/28119925 http://dx.doi.org/10.1155/2016/2904832 |
work_keys_str_mv | AT korokorofrancioli geneticstructureanddrugsusceptibilitypatternsofmycobacteriumtuberculosiscomplexstrainsresponsibleofhumanpulmonarytuberculosisinthemajorrearingregionincameroon AT umboockalphonse geneticstructureanddrugsusceptibilitypatternsofmycobacteriumtuberculosiscomplexstrainsresponsibleofhumanpulmonarytuberculosisinthemajorrearingregionincameroon AT kaiyvenafileslie geneticstructureanddrugsusceptibilitypatternsofmycobacteriumtuberculosiscomplexstrainsresponsibleofhumanpulmonarytuberculosisinthemajorrearingregionincameroon AT noeskejuergen geneticstructureanddrugsusceptibilitypatternsofmycobacteriumtuberculosiscomplexstrainsresponsibleofhumanpulmonarytuberculosisinthemajorrearingregionincameroon AT gutierrezcristina geneticstructureanddrugsusceptibilitypatternsofmycobacteriumtuberculosiscomplexstrainsresponsibleofhumanpulmonarytuberculosisinthemajorrearingregionincameroon AT kuabanchristopher geneticstructureanddrugsusceptibilitypatternsofmycobacteriumtuberculosiscomplexstrainsresponsibleofhumanpulmonarytuberculosisinthemajorrearingregionincameroon AT etoafrancoisxavier geneticstructureanddrugsusceptibilitypatternsofmycobacteriumtuberculosiscomplexstrainsresponsibleofhumanpulmonarytuberculosisinthemajorrearingregionincameroon AT eyangohsarairene geneticstructureanddrugsusceptibilitypatternsofmycobacteriumtuberculosiscomplexstrainsresponsibleofhumanpulmonarytuberculosisinthemajorrearingregionincameroon |