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Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone

Benzene is metabolized to hydroquinone in liver and subsequently transported to bone marrow for further oxidization to 1,4-benzoquinone (1,4-BQ), which may be related to the leukemia and other blood disorders. In the present study, we investigated the proteome profiles of human primary bone marrow m...

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Detalles Bibliográficos
Autores principales: Wu, Wei, Zhang, Ling, Zhang, Min, Zhu, Li-Jin, Xia, Hai-Ling, Lou, Jian-Lin, Liu, Jia-Ren, Xiao, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5227119/
https://www.ncbi.nlm.nih.gov/pubmed/28119923
http://dx.doi.org/10.1155/2016/2789245
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author Wu, Wei
Zhang, Ling
Zhang, Min
Zhu, Li-Jin
Xia, Hai-Ling
Lou, Jian-Lin
Liu, Jia-Ren
Xiao, Yun
author_facet Wu, Wei
Zhang, Ling
Zhang, Min
Zhu, Li-Jin
Xia, Hai-Ling
Lou, Jian-Lin
Liu, Jia-Ren
Xiao, Yun
author_sort Wu, Wei
collection PubMed
description Benzene is metabolized to hydroquinone in liver and subsequently transported to bone marrow for further oxidization to 1,4-benzoquinone (1,4-BQ), which may be related to the leukemia and other blood disorders. In the present study, we investigated the proteome profiles of human primary bone marrow mesenchymal stem cells (hBM-MSCs) treated by 1,4-BQ. We identified 32 proteins that were differentially expressed. Two of them, HSP27 and Vimentin, were verified at both mRNA and protein levels and their cellular localization was examined by immunofluorescence. We also found increased mRNA level of RAP1GDS1, a critical factor of metabolism that has been identified as a fusion partner in various hematopoietic malignancies. Therefore, these differentially expressed proteins can play important roles in benzene-mediated hematoxicity.
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spelling pubmed-52271192017-01-24 Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone Wu, Wei Zhang, Ling Zhang, Min Zhu, Li-Jin Xia, Hai-Ling Lou, Jian-Lin Liu, Jia-Ren Xiao, Yun Biomed Res Int Research Article Benzene is metabolized to hydroquinone in liver and subsequently transported to bone marrow for further oxidization to 1,4-benzoquinone (1,4-BQ), which may be related to the leukemia and other blood disorders. In the present study, we investigated the proteome profiles of human primary bone marrow mesenchymal stem cells (hBM-MSCs) treated by 1,4-BQ. We identified 32 proteins that were differentially expressed. Two of them, HSP27 and Vimentin, were verified at both mRNA and protein levels and their cellular localization was examined by immunofluorescence. We also found increased mRNA level of RAP1GDS1, a critical factor of metabolism that has been identified as a fusion partner in various hematopoietic malignancies. Therefore, these differentially expressed proteins can play important roles in benzene-mediated hematoxicity. Hindawi Publishing Corporation 2016 2016-12-29 /pmc/articles/PMC5227119/ /pubmed/28119923 http://dx.doi.org/10.1155/2016/2789245 Text en
spellingShingle Research Article
Wu, Wei
Zhang, Ling
Zhang, Min
Zhu, Li-Jin
Xia, Hai-Ling
Lou, Jian-Lin
Liu, Jia-Ren
Xiao, Yun
Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone
title Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone
title_full Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone
title_fullStr Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone
title_full_unstemmed Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone
title_short Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone
title_sort proteome changes of human bone marrow mesenchymal stem cells induced by 1,4-benzoquinone
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5227119/
https://www.ncbi.nlm.nih.gov/pubmed/28119923
http://dx.doi.org/10.1155/2016/2789245
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