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Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats

Stem cell transplantation and low-energy shock-wave therapy (LESWT) have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in diabetic erectile dysfunction models, the number of visible stem cells was rather low a...

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Autores principales: Shan, Hai-Tao, Zhang, Hai-Bo, Chen, Wen-Tao, Chen, Feng-Zhi, Wang, Tao, Luo, Jin-Tai, Yue, Min, Lin, Ji-Hong, Wei, An-Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5227668/
https://www.ncbi.nlm.nih.gov/pubmed/27427555
http://dx.doi.org/10.4103/1008-682X.184271
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author Shan, Hai-Tao
Zhang, Hai-Bo
Chen, Wen-Tao
Chen, Feng-Zhi
Wang, Tao
Luo, Jin-Tai
Yue, Min
Lin, Ji-Hong
Wei, An-Yang
author_facet Shan, Hai-Tao
Zhang, Hai-Bo
Chen, Wen-Tao
Chen, Feng-Zhi
Wang, Tao
Luo, Jin-Tai
Yue, Min
Lin, Ji-Hong
Wei, An-Yang
author_sort Shan, Hai-Tao
collection PubMed
description Stem cell transplantation and low-energy shock-wave therapy (LESWT) have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in diabetic erectile dysfunction models, the number of visible stem cells was rather low and decreased quickly. LESWT could recruit endogenous stem cells to the cavernous body and improve the microenvironment in diabetic cavernous tissue. Thus, we deduced that LESWT might benefit transplanted stem cell survival and improve the effects of stem cell transplantation. In this research, 42 streptozotocin-induced diabetic rats were randomized into four groups: the diabetic group (n = 6), the LESWT group (n = 6), the bone marrow-derived mesenchymal stem cell (BMSC) transplantation group (n = 15), and the combination of LESWT and BMSC transplantation group (n = 15). One and three days after BMSC transplantation, three rats were randomly chosen to observe the survival numbers of BMSCs in the cavernous body. Four weeks after BMSC transplantation, the following parameters were assessed: the surviving number of transplanted BMSCs in the cavernous tissue, erectile function, real-time polymerase chain reaction, and penile immunohistochemical assessment. Our research found that LESWT favored the survival of transplanted BMSCs in the cavernous body, which might be related to increased stromal cell-derived factor-1 expression and the enhancement of angiogenesis in the diabetic cavernous tissue. The combination of LESWT and BMSC transplantation could improve the erectile function of diabetic erectile function rats more effectively than LESWT or BMSC transplantation performed alone.
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spelling pubmed-52276682017-02-03 Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats Shan, Hai-Tao Zhang, Hai-Bo Chen, Wen-Tao Chen, Feng-Zhi Wang, Tao Luo, Jin-Tai Yue, Min Lin, Ji-Hong Wei, An-Yang Asian J Androl Original Article Stem cell transplantation and low-energy shock-wave therapy (LESWT) have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in diabetic erectile dysfunction models, the number of visible stem cells was rather low and decreased quickly. LESWT could recruit endogenous stem cells to the cavernous body and improve the microenvironment in diabetic cavernous tissue. Thus, we deduced that LESWT might benefit transplanted stem cell survival and improve the effects of stem cell transplantation. In this research, 42 streptozotocin-induced diabetic rats were randomized into four groups: the diabetic group (n = 6), the LESWT group (n = 6), the bone marrow-derived mesenchymal stem cell (BMSC) transplantation group (n = 15), and the combination of LESWT and BMSC transplantation group (n = 15). One and three days after BMSC transplantation, three rats were randomly chosen to observe the survival numbers of BMSCs in the cavernous body. Four weeks after BMSC transplantation, the following parameters were assessed: the surviving number of transplanted BMSCs in the cavernous tissue, erectile function, real-time polymerase chain reaction, and penile immunohistochemical assessment. Our research found that LESWT favored the survival of transplanted BMSCs in the cavernous body, which might be related to increased stromal cell-derived factor-1 expression and the enhancement of angiogenesis in the diabetic cavernous tissue. The combination of LESWT and BMSC transplantation could improve the erectile function of diabetic erectile function rats more effectively than LESWT or BMSC transplantation performed alone. Medknow Publications & Media Pvt Ltd 2017 2016-07-12 /pmc/articles/PMC5227668/ /pubmed/27427555 http://dx.doi.org/10.4103/1008-682X.184271 Text en Copyright: © 2017 AJA, SIMM & SJTU http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Shan, Hai-Tao
Zhang, Hai-Bo
Chen, Wen-Tao
Chen, Feng-Zhi
Wang, Tao
Luo, Jin-Tai
Yue, Min
Lin, Ji-Hong
Wei, An-Yang
Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats
title Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats
title_full Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats
title_fullStr Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats
title_full_unstemmed Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats
title_short Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats
title_sort combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5227668/
https://www.ncbi.nlm.nih.gov/pubmed/27427555
http://dx.doi.org/10.4103/1008-682X.184271
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