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Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats
The present study evaluated the protective effect of selenium against cisplatin-induced nasopharyngeal cancer in the cardiac tissue of adult rats. Male Wistar rats were divided into 4 groups of 6 rats each. Different combinations of selenium and cisplatin were administered for 45 days, following whi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5228205/ https://www.ncbi.nlm.nih.gov/pubmed/28101235 http://dx.doi.org/10.3892/ol.2016.5346 |
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author | Zhu, Kan Jiang, Lei Chu, Yun Zhang, Yong-Sheng |
author_facet | Zhu, Kan Jiang, Lei Chu, Yun Zhang, Yong-Sheng |
author_sort | Zhu, Kan |
collection | PubMed |
description | The present study evaluated the protective effect of selenium against cisplatin-induced nasopharyngeal cancer in the cardiac tissue of adult rats. Male Wistar rats were divided into 4 groups of 6 rats each. Different combinations of selenium and cisplatin were administered for 45 days, following which the animals were sacrificed and the nasopharyngeal tissue was surgically removed. Lipid peroxidation, reduced glutathione, and the antioxidant enzymes superoxide dismutase (SOD), catalase and lactate dehydrogenase (LDH) were measured. Apoptotic-related gene (p53, bax and caspase 3) mRNA expression was determined using quantitative polymerase chain reaction. Caspase 3 activity was also measured in all the groups. The results demonstrated that selenium significantly reduced the levels of malondialdehyde. The levels of glutathione, SOD, LDH and catalase significantly increased following selenium treatment. Relative mRNA expression (p53, bax and caspase 3) was significantly reduced in the cisplatin-treated rats, but it significantly increased following selenium treatment. The anticancer activity of selenium was also investigated in HK1cells. Fluorescence and confocal microscopy were used to analyze apoptosis and reactive oxygen species. The protective effect of selenium was also evident through caspase 3 activity, which significantly increased following selenium treatment. Taken together, these results indicate that selenium may be beneficial against cisplatin-induced nasopharyngeal cancer. |
format | Online Article Text |
id | pubmed-5228205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-52282052017-01-18 Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats Zhu, Kan Jiang, Lei Chu, Yun Zhang, Yong-Sheng Oncol Lett Articles The present study evaluated the protective effect of selenium against cisplatin-induced nasopharyngeal cancer in the cardiac tissue of adult rats. Male Wistar rats were divided into 4 groups of 6 rats each. Different combinations of selenium and cisplatin were administered for 45 days, following which the animals were sacrificed and the nasopharyngeal tissue was surgically removed. Lipid peroxidation, reduced glutathione, and the antioxidant enzymes superoxide dismutase (SOD), catalase and lactate dehydrogenase (LDH) were measured. Apoptotic-related gene (p53, bax and caspase 3) mRNA expression was determined using quantitative polymerase chain reaction. Caspase 3 activity was also measured in all the groups. The results demonstrated that selenium significantly reduced the levels of malondialdehyde. The levels of glutathione, SOD, LDH and catalase significantly increased following selenium treatment. Relative mRNA expression (p53, bax and caspase 3) was significantly reduced in the cisplatin-treated rats, but it significantly increased following selenium treatment. The anticancer activity of selenium was also investigated in HK1cells. Fluorescence and confocal microscopy were used to analyze apoptosis and reactive oxygen species. The protective effect of selenium was also evident through caspase 3 activity, which significantly increased following selenium treatment. Taken together, these results indicate that selenium may be beneficial against cisplatin-induced nasopharyngeal cancer. D.A. Spandidos 2016-12 2016-11-02 /pmc/articles/PMC5228205/ /pubmed/28101235 http://dx.doi.org/10.3892/ol.2016.5346 Text en Copyright: © Zhu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhu, Kan Jiang, Lei Chu, Yun Zhang, Yong-Sheng Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats |
title | Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats |
title_full | Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats |
title_fullStr | Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats |
title_full_unstemmed | Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats |
title_short | Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats |
title_sort | protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5228205/ https://www.ncbi.nlm.nih.gov/pubmed/28101235 http://dx.doi.org/10.3892/ol.2016.5346 |
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