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Upregulated microRNA-143 inhibits cell proliferation in human nasopharyngeal carcinoma

The aim of the present study was to investigate the possible functions and mechanism of microRNA (miR)-143 in cell proliferation of human nasopharyngeal carcinoma (NPC). The expression of miR-143 in NPC cells and tissues was investigated using reverse transcription-quantitative polymerase chain reac...

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Autores principales: He, Benfu, Xu, Zhe, Chen, Jinzhang, Zheng, Dayong, Li, Aimin, Zhang, Luo-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5228360/
https://www.ncbi.nlm.nih.gov/pubmed/28105209
http://dx.doi.org/10.3892/ol.2016.5363
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author He, Benfu
Xu, Zhe
Chen, Jinzhang
Zheng, Dayong
Li, Aimin
Zhang, Luo-Sheng
author_facet He, Benfu
Xu, Zhe
Chen, Jinzhang
Zheng, Dayong
Li, Aimin
Zhang, Luo-Sheng
author_sort He, Benfu
collection PubMed
description The aim of the present study was to investigate the possible functions and mechanism of microRNA (miR)-143 in cell proliferation of human nasopharyngeal carcinoma (NPC). The expression of miR-143 in NPC cells and tissues was investigated using reverse transcription-quantitative polymerase chain reaction. Cell viability assay, colony formation assay and flow cytometry were used to examine the cell proliferative ability and tumorigenicity. The expression levels of p21(Cip1), p27(Kip1), cyclin D1, phosphorylated (p)-retinoblastoma protein (Rb), Rb and cyclin-dependent kinase (CDK) 6 were determined by western blotting. Luciferase assay was used to confirm whether CDK6 was a direct target of miR-143. miR-143 was downregulated in NPC cell lines and tissues. Overexpression of miR-143 in NPC CNE1 cells inhibited proliferation, tumorigenicity and cell cycle progression. Additionally, ectopic expression of miR-143 downregulated cyclin D1 and p-Rb expression, and upregulated p21(Cip1) and p27(Kip1) expression, which subsequently inhibited NPC cell proliferation. It was also observed that CDK6 was a direct target of miR-143, which downregulated CDK6 expression. CDK6 suppression by miR-143 was associated with dysregulated expression of p21(Cip1), p27(Kip1), cyclin D1 and p-Rb, thereby serving an essential role in NPC cell growth. Our findings suggest that miR-143 inhibits proliferation by targeting CDK6, and may aid to identify new targets for anti-oncomiRs.
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spelling pubmed-52283602017-01-19 Upregulated microRNA-143 inhibits cell proliferation in human nasopharyngeal carcinoma He, Benfu Xu, Zhe Chen, Jinzhang Zheng, Dayong Li, Aimin Zhang, Luo-Sheng Oncol Lett Articles The aim of the present study was to investigate the possible functions and mechanism of microRNA (miR)-143 in cell proliferation of human nasopharyngeal carcinoma (NPC). The expression of miR-143 in NPC cells and tissues was investigated using reverse transcription-quantitative polymerase chain reaction. Cell viability assay, colony formation assay and flow cytometry were used to examine the cell proliferative ability and tumorigenicity. The expression levels of p21(Cip1), p27(Kip1), cyclin D1, phosphorylated (p)-retinoblastoma protein (Rb), Rb and cyclin-dependent kinase (CDK) 6 were determined by western blotting. Luciferase assay was used to confirm whether CDK6 was a direct target of miR-143. miR-143 was downregulated in NPC cell lines and tissues. Overexpression of miR-143 in NPC CNE1 cells inhibited proliferation, tumorigenicity and cell cycle progression. Additionally, ectopic expression of miR-143 downregulated cyclin D1 and p-Rb expression, and upregulated p21(Cip1) and p27(Kip1) expression, which subsequently inhibited NPC cell proliferation. It was also observed that CDK6 was a direct target of miR-143, which downregulated CDK6 expression. CDK6 suppression by miR-143 was associated with dysregulated expression of p21(Cip1), p27(Kip1), cyclin D1 and p-Rb, thereby serving an essential role in NPC cell growth. Our findings suggest that miR-143 inhibits proliferation by targeting CDK6, and may aid to identify new targets for anti-oncomiRs. D.A. Spandidos 2016-12 2016-11-08 /pmc/articles/PMC5228360/ /pubmed/28105209 http://dx.doi.org/10.3892/ol.2016.5363 Text en Copyright: © He et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
He, Benfu
Xu, Zhe
Chen, Jinzhang
Zheng, Dayong
Li, Aimin
Zhang, Luo-Sheng
Upregulated microRNA-143 inhibits cell proliferation in human nasopharyngeal carcinoma
title Upregulated microRNA-143 inhibits cell proliferation in human nasopharyngeal carcinoma
title_full Upregulated microRNA-143 inhibits cell proliferation in human nasopharyngeal carcinoma
title_fullStr Upregulated microRNA-143 inhibits cell proliferation in human nasopharyngeal carcinoma
title_full_unstemmed Upregulated microRNA-143 inhibits cell proliferation in human nasopharyngeal carcinoma
title_short Upregulated microRNA-143 inhibits cell proliferation in human nasopharyngeal carcinoma
title_sort upregulated microrna-143 inhibits cell proliferation in human nasopharyngeal carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5228360/
https://www.ncbi.nlm.nih.gov/pubmed/28105209
http://dx.doi.org/10.3892/ol.2016.5363
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